Induction of protective T-helper 1 immune responses against Echinococcus granulosus in mice by a multi-T-cell epitope antigen based on five proteins

Esmaelizad, Majid; Ahmadian, Gholamreza; Aghaiypour, Khosrow; Shamsara, Mehdi; Paykari, H.; Tebianian, Majid

doi:10.1590/s0074-0276108042013003

Cited by 8 publications

(7 citation statements)

References 19 publications

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“…The recombinant vaccine was developed from the G1 genotype of E. granulosus , and protectivity against other genotypes is unclear . The use of chimeric protein constructs of different epitopes may constitute a different approach .…”

Section: Immune Response In the Intermediate Hostmentioning

confidence: 99%

“…It is generally proposed by the literature that a Th2 response would favour parasite establishment as opposite to a parasite‐killing Th1 response. However, the picture appears much more complex, as (i) often a mixed Th1/Th2 response is present in infected hosts, (ii) regulatory mechanisms are likely to control both Th1 and Th2 parasite‐killing effector mechanisms (for example, Tregs have been recently described locally increased as early as 5 days post‐PSC injection ), and (iii) it is often difficult to say whether a particular type of immune response is the cause or the result of a certain infection status . Some evidence supports an increased parasite‐killing effect when Th1 type responses are boosted.…”

Section: Immune Response In the Intermediate Hostmentioning

confidence: 99%

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The intermediate host immune response in cystic echinococcosis

Tamarozzi

Mariconti²,

Neumayr

et al. 2016

Parasite Immunology

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Cystic echinococcosis (CE) is a chronic, complex and neglected zoonotic infection. In most cases, CE cysts and the intermediate host co-habit for a long time in the absence of symptoms and elicit very little inflammation. However, the immune interplay between the parasite and the host is complex, encompassing effective parasite-killing immune mechanisms implemented by the host, which in turn are modulated by the parasite. The immune response to the parasite has been exploited for the diagnosis of the disease and for the development of an effective vaccine to use in the natural intermediate host, but the mechanisms of parasite killing and immunomodulation are still unknown. Here, we reviewed the immune effector mechanisms and the strategies of immune evasion in the intermediate host.

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Section: Immune Response In the Intermediate Hostmentioning

confidence: 99%

Section: Immune Response In the Intermediate Hostmentioning

confidence: 99%

The intermediate host immune response in cystic echinococcosis

Tamarozzi

Mariconti²,

Neumayr

et al. 2016

Parasite Immunology

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“…2 The proliferation of specific B-cell lymphocytes from mice immunized with Emy162; ***P < 0.001 Fig. 3 The ELISA test of the B-cell antigen peptide of Emy162 acting with serum of AE patients; ***P < 0.001 polypeptide vaccine [21], and others, have been developed in recent years. Zheng et al [22] suggested that prenyltransferase, together with prenylcysteine oxidase, might act as a novel drug to target hydatidosis.…”

Section: Discussionmentioning

confidence: 99%

Prediction and Identification of Epitopes in the Emy162 Antigen of Echinococcus multilocularis

et al. 2020

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Introduction Alveolar echinococcosis (AE) is a zoonotic disease caused by the parasitism of Echinococcus multilocularis larvae in the intermediate host or the final host. This study aims to identify and analyze the B-cell and T-cell (Th1, Th2 and Th17) epitopes of E. multilocularis antigen Emy162. Methods (1) The secondary structural characteristics of the Emy162 protein were predicted by bioinformatics software to further predict the potential T-and B-cell epitopes. (2) The dominant antigen epitopes were detected by ELISA through the reaction of patient serum with small B-cell antigen peptide and assessing the proliferation of splenic lymphocytes of mice immunized with Emy162. (3) The expression of cytokines in splenic lymphocytes of mice stimulated by small T-cell antigen peptides was detected by ELISA, ELISpot and flow cytometry to enable the identification of the T-cell epitopes. Results (1) The high-scored T-cell epitopes were located at positions E7-13, E36-41, E80-89, E87-96, E97-106 and E129-139, while B-cell epitopes were located at positions E7-13, E19-27, E28-36, E37-48, E78-83, E101-109, E112-121 and E129-139. (2) The three advanced antigen epitopes of Emy162 were E19-27, E112-121 and E129-139. (3) The four Th1 advanced antigen epitopes of Emy162 were E7-13, E36-41, E80-89 and E129-139. The three Th2 advanced antigen epitopes were E36-41, E87-96 and E97-106. The three Th17 advanced antigen epitopes were E36-41, E87-96 and E97-106. Conclusion (1) The Emy162 protein has advanced antigenicity and numerous potential epitopes. Six T-cell and eight B-cell dominant epitopes were revealed using bioinformatics methods. (2) There are three dominant B-cell epitopes, four dominant Th1 epitopes, three dominant Th2 epitopes, and three dominant Th17 epitopes in the Emy162 antigen.

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“…Some research demonstrated the potential for successful transfer of immunity against E. granulosus to lambs via colostrum; this study showed that exposed ewes to three oral infections with E. granulosus eggs, as well as immunized ewes with E. granulosus oncospheral antigens, transferred a remarkable level of immunity to lambs through colostrum. However, the natural immunity is often concomitant with some degrees of injury to the internal organs including liver, lung, heart, and spleen as well as economics detriments [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens

Valizadeh¹,

Behzad²,

Badirzadeh³

et al. 2017

Vet World

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Aim:Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine.Materials and Methods:Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 µg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.Results:After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).Conclusion:The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.

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Induction of protective T-helper 1 immune responses against Echinococcus granulosus in mice by a multi-T-cell epitope antigen based on five proteins

Cited by 8 publications

References 19 publications

The intermediate host immune response in cystic echinococcosis

The intermediate host immune response in cystic echinococcosis

Prediction and Identification of Epitopes in the Emy162 Antigen of Echinococcus multilocularis

Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens

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