Oviposition behavior of wild Zabrotes subfasciatus (Coleoptera, Chrysomelidae) females deprived of the host Phaseolus vulgaris (Fabaceae)

Abstract

Oviposition behavior of wild Zabrotes subfasciatus (Coleoptera, Chrysomelidae) females deprived of the host Phaseolus vulgaris (Fabaceae)LuizaABSTRACT. The insects oviposition behavior is fundamental to study population dynamics, life history evolution, insect-plant and parasitoid-host interactions. Zabrotes subfasciatus (Boheman, 1833) females oviposition behavior in the presence and absence of a host is unknown. The main objective of this study was to describe in detail the oviposition behavior of host deprived or non-deprived females, and observe how the several situations of deprivation (days without host) influence oviposition. Six groups were assembled, three deprived of the host (for 2, 5 and 8 days) and three control groups (with host), each containing one newly-emerged couple (0-24h) of wild Z. subfasciatus, The non-deprived (control) groups received the hosts every day (5 bean seeds Phaseolus vulgaris (Fabaceae)) and the others were deprived for 2, 5 and 8 days, respectively. For each group 12 repetitions were made. Consequently, 12 couples were host deprived during two days, 12 couples were host deprived during five days and 12 couples were host deprived during eight days. When the seeds of the deprived groups were added the experiments started. There was a control group for each deprived group. The experiments and the insects were maintained at constant temperature 29 ± 2°C and 70-80% relative humidity. At 15 minutes interval, the number of times the females manifested the different categories of behavior was observed (frequency). The behavior categories were: rest inside the box, locomotion, resource exploration (seeds), copulation and oviposition. The deprived females stayed most of the time in contact with the host to carry out oviposition, while the non-deprived (control) females spent most of the time at rest. This was observed in all the deprivation times. The results show that host deprivation influences the oviposition behavior of the studied species and also shows the flexibility in the oviposition strategies that these females present when the environment changes (absence and presence of resources). KEYWORDS. Host deprivation, oviposition behavior, Bruchinae. RESUMO. Comportamento de ovoposição de fêmeas selvagens de Zabrotes subfasciatus (Coleoptera, Chrysomelidae) privadas do hospedeiro Phaseolus vulgaris (Fabaceae). O comportamento de oviposição de insetos é fundamental em pesquisas de dinâmica populacional, evolução da história de vida, interações inseto-planta e parasitóide-hospedeiro. O comportamento de oviposição de fêmeas deZabrotes subfasciatus (Boheman, 1833) na presença e ausência de hospedeiro é desconhecido. O principal objetivo deste trabalho foi verificar de que modo as várias situações de privação temporária de hospedeiro influenciam a oviposição. Foram montados 6 grupos, sendo 3 privados de hospedeiro (por 2, 5 e 8 dias) e 3 controles (com hospedeiro), cada um contendo um casal recém-emergido (0-24 h) de Z. subfasciatus selvagem. Apenas os grupos controles receberam os hosp...

Multigenerational experimental simulation of climate change on an economically important insect pest

“…Second, since Z. subfascia tus is a capital breeder, the physiological assignment of resources during larval development is shifted from egg number to egg quality. Third, eggs and larvae simply develop better in the conditions we simulated as this species has a relatively wide temperature tolerance but a thermal optimum of 27–30°C (Sperandio & Zucoloto, 2009). In other words, our data suggest that augmented temperature and CO 2 have the potential to lead to an increased fitness as the females seem to change their ovipositing strategy by laying fewer eggs and the developing larvae show a higher probability to reach the imago stage.…”

Expression of the Arcelin 1 gene from Phaseolus vulgaris L. in cowpea seeds (Vigna unguiculata L.) confers bruchid resistance

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