Capacidade antioxidante e triagem farmacológica de extratos brutos de folhas de Byrsonima crassifolia e de Inga edulis

Abstract

ResumoCom o objetivo de avaliar o efeito de duas espécies amazônicas em doenças relacionadas aos processos de oxidação, determinouse a capacidade antioxidante (método Oxygen Radical Absorbance Capacity), o teor de polifenóis totais (método Folin-Ciocalteu -Pt), bem como os efeitos farmacológicos in vitro (efeito antiproliferativo) e in vivo (antinociceptivo, antiinflamatório, antiulcerogênico) dos extratos hidroalcoólicos (65:35; v/v; etanol:água) ) de sua administração oral. nos modelos de inflamação houve somente redução do edema para ie na concentração de 500 mg kg -1 . os extratos das duas espécies reduziram as lesões ulcerativas produzidas por etanol em até 84% (P < 0,05), sugerindo uma possível ligação com a atividade antioxidante observada e indicando a necessidade de estudos para a elucidação do mecanismo de ação envolvido. PalavRas-Chaves: amazônia; oxygen radical absorbance capacity; Polifenóis; Propriedades farmacológicas. Antioxidant capacity and pharmacologic screening of crude extracts ofByrsonima crassifolia and Inga edulis leaves abstRaCt in order to evaluate the effect of two amazonian species on chronic diseases linked with the oxidative processes, we performed antioxidant capacity analyses (oxygen Radical absorbance Capacity -oRaC and Folin-Ciocalteu -Pt assays) and pharmacological effects in vitro (antiproliferative effect) and in vivo (antinociceptive, antiinflammatory, antiulcerogenic effects) for ethanolic extracts (65:35; v/v; ethanol:water) from Byrsonima crassifolia (BC) and Inga edulis (ie) leaves. Both BC and ie extracts showed high oRaC values (1,422 and 694 mmol of trolox equivalent/g of dry leaf, respectively) and high Pt contents (35.93 and 24.50 mg gallic acid equivalent g -1 dry leaf, respectively). The oRaC values had no correlation with Pt, suggesting the presence of other chemical groups in the antioxidant activity value. The two extracts did not present significant antiproliferative activity on nine lines of human tumor cells, and cytotoxic effect was detected only at the highest concentration. The antinociceptive effect was investigated using the hot plate test, and ie extract presented a longer latency (P < 0.05) 30 and 60 min after oral administration. The antiinflammatory activity was only observed at the highest concentration, suggesting that the antinociceptive effect observed was not due to the antiinflammatory effect. The extracts of both species reduced the ulcerative lesions produced by ethanol up to 84% (P < 0.05), suggesting a relation with the antioxidant capacity. more studies are necessary to elucidate the mechanisms of action involved on antiulcerative effects.

Phytochemistry, antioxidant potential and antifungal of Byrsonima crassifolia on soil phytopathogen control

“…This demonstrates that among the total phenols presented in barks, great part of these belong to the flavonoid group. Studying barks of B. crassifolia, Silva and Rogez (2013), found similar value to the one observed in this paper (208.1 ± 9.7 mg g-1) and superior to the one found by Pompeu et al (2012) that was 58.1 mg g -1 of total phenols for the hydroethanol crude extract of dried leaves of B. crassifolia collected at the region of Belem, PA, Brazil. For the crude extract of aerial parts of B. gardneriana, the values were superiors (425.105 ± 8.523 mg EAG g-1) (Rolim et al, 2013), which suggests that Byrsonima species have high levels of total phenols in their composition.…”

“…These results point that the extract have antioxidant activity, justified by the presence of phenolic compounds and flavonoids, known by their capacity to redeem free radicals and inhibit possible oxidant damages. The same antioxidant activity of hidro ethanolic extract of Bysonima crassifolia leaves was previously described by (Pompeu et al, 2012) and for others species of the same genre by (Rolim et al, 2013).…”

Nutritional, antinutritional and phytochemical status of okra leaves (Abelmoschus esculentus) subjected to different processes

“…The content of total phenolics in A. esculentus extract was 19.27 ± 0.9 mg GAE/g. The content of total phenolics in A. esculentu was lower than that found in other leaves; T. brasiliensis (38.53 ± 0.63 GAE/g)), C. macrophyllum (66.14 ± 3.56 GAE/g) (Sousa et al, 2007); B. crassifolia (35.93 mg GAE/g), I. edulis (24.50 mg GAE/g) (Pompeu et al, 2012), but higher than values found in fresh okra leaves (less than 0.15 mg GAE/g), seeds (2.13 µg/g) and root (0.55 µg/g) (Leiote et al, 2009), in Cosmos caudatus (18.83 mg GAE/g), Centella asiatica (7.79 mg GAE/g) and Oenanthe javanica leaves (7.41 mg GAE/g) (Huda-Faujan et al, 2009). For some derivatives of phenolic acids, antioxidant activity has been reported, except for those of high molecular weight, such as tannins.…”

Chemodiversity, Bioactivity and Chemosystematics of the Genus Inga (FABACEAE): A Brief Review

“…17 Estudos realizados com o extrato hidroalcoólico das folhas de I. edulis demonstrou um elevado teor de compostos fenólicos e uma alta capacidade antioxidante, bem como uma significativa atividade antiulcerogênica. 18 O …”