2012
DOI: 10.1590/s0037-86822012000400018
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Abstract: Introduction:The correlation between the immunological assay and the antibody titer can offer a tool for the experimental analysis of different phases of the disease. Methods: Two simple immunological assays for Schistosoma mansoni in mice sera samples based on specific IgG detection for worms soluble antigens and eggs soluble antigens were standardized and evaluated in our laboratory. Fifty mice were used in negative and positive groups and the results obtained by enzyme-linked immunosorbent assays (ELISA) as… Show more

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Cited by 6 publications
(4 citation statements)
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“…5), similar to previously reported studies [56]. Among the SmTR antigens, SmTR8 showed the highest correlation with SWAP and SEA (Figs.…”
Section: Correlation Between Smtrs Sea and Swapsupporting
confidence: 90%
“…5), similar to previously reported studies [56]. Among the SmTR antigens, SmTR8 showed the highest correlation with SWAP and SEA (Figs.…”
Section: Correlation Between Smtrs Sea and Swapsupporting
confidence: 90%
“…mansoni adult worm soluble antigen)–in-house ELISA [23] was performed on all mice to detect immunoglobulin G (IgG) against worms.…”
Section: Methodsmentioning
confidence: 99%
“…Although the antigenic composition is not the same as the live worm, the use of SWAP or SWA constitutes a valuable experimental tool to evaluate many aspects of immune responses promoted by different host cells (Xu et al ., 2014 ; Zhu et al ., 2014 ). This antigen is the easiest to obtain and is essentially an extract based on Tris-HCl or phosphate-buffered saline from mixed male and female worms and prepared in various ways, either by homogenization, sonication or freeze/thaw (or a combination of these) (Grenfell et al ., 2012 ; Neves et al ., 2015 ). Some studies have demonstrated that SWAP could induce an M1-like profile (Xu et al ., 2014 ; Zhu et al ., 2014 ).…”
Section: Worm Antigens Can Induce An M1 or M2 Profilementioning
confidence: 99%