Atividade larvicida do extrato bruto enzimático do fungo Duddingtonia flagras sobre larvas de primeiro estádio de Angiostrongylus vasorum

Braga, Fábio Ribeiro; Araújo, Juliana Milani; Tavela, Alexandre de Oliveira; Araújo, Jackson Víctor de; Soares, Filippe Elias de Freitas; Gênier, Hugo Leonardo André; Lima, Walter dos Santos; Mozzer, Lanuze Rose; Queiróz, José Humberto de

doi:10.1590/s0037-86822011000300024

Cited by 10 publications

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References 11 publications

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“…However, there is an increasing need to elucidate the real possibility of using these extracts under partially natural conditions, such as coprocultures. Moreover, the species Duddingtonia flagrans produces extracellular enzymes that are observed in its crude extract, which cause hydrolysis of the cuticle of nematodes, thus allowing this fungus to also act inside the nematode and leading to its destruction (BRAGA et al, 2011c).…”

Section: Introductionmentioning

confidence: 99%

“…1 filter paper at 4 °C. The methodology for producing and obtaining the crude extract was based on the study by Braga et al (2011c).…”

Section: Introductionmentioning

confidence: 99%

“…In this context, some studies have shown that use of nematophagous fungi may be a promising alternative for reducing the quantity of infective forms present in the environment, given that conidia, chlamydospores and even mycelial masses have been used successfully (CHARLES et al, 1995;CASTRO et al, 2003;CAMPOS et al, 2008;BRAGA et al, 2011c;SILVA et al, 2010). On the other hand, crude extract has recently also been used as an approach towards combating the eggs and/or larvae of gastrointestinal nematode parasites (BRAGA et al, 2011a, c).…”

Section: Introductionmentioning

confidence: 99%

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Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures

Braga

Araújo

Soares

et al. 2013

Rev. Bras. Parasitol. Vet.

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The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722) on infective larvae (L 3 ) of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001); group 2: 10 mL of crude extract of AC001 with 10 mM of Ca 2+ ; group 3: 10 mL of crude extract of D. flagrans (CG722); group 4: 10 mL of crude extract of CG722 with 10 mM of Ca 2+ ; and group 5: control group (distilled water). The third-stage larvae (L 3 ) were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L 3 , with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05). The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungus D. flagrans could be used for controlling cyathostomin L 3 , and suggested that at least one protease of approximately 38 kDa was present.

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Section: Introductionmentioning

confidence: 99%

“…1 filter paper at 4 °C. The methodology for producing and obtaining the crude extract was based on the study by Braga et al (2011c).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures

Braga

Araújo

Soares

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…Man acquires the infection after ingestion of the intermediate or paratenic host and through ingestion of rainwater in the field where this nematode is present (Wang et al, 2007). In this context, the use of nematophagous fungi of the genera Duddingtonia, Monacrosporium and Arthrobotrys has been successfully tested, including on the Angiostrongylus genus, specifically on the A. vasorum species (Braga et al, , 2011Soares et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

First report of the activity of predatory fungi on Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) first-stage larvae

et al. 2013

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The nematode Angiostrongylus cantonensis causes eosinophilic meningoencephalitis in humans and thus alternative methods of control should be studied. The objective of this work was to evaluate the predatory capacity of eight fungal isolates of the species Duddingtonia flagrans (AC001, CG768 and CG722), Monacrosporium thaumasium (NF34), M. sinense (SF53) and Arthrobotrys robusta (I31), A. cladodes (CG719) and A. conoides (I40) on first-stage larvae (L₁) of A. cantonensis under laboratory conditions. The treated groups contained 1000 conidia of the fungal isolates and 1000 A. cantonensis L₁ in Petri dishes containing 2% water-agar medium (2% WA). The control group (without fungi) contained only 1000 A. cantonensis L₁ in 2% WA. Evidence of predation was observed at the end of 7 days. Percentage reductions in L₁ were: AC001, 82.8%; CG768, 71.0%; CG722, 72.8%; NF34, 86.7%; SF53, 89.7%; I40, 48.3%; CG719, 84.7%; and I31, 80.4%. No significant difference was observed (p>0.01) between the actions of the isolates used; however, a difference was noted (p<0.01) in relation to the control group. The results of the present work, confirm previous reports of the effectiveness of the fungi D. flagrans, M. thaumasium, M. sinense and A. robusta in controlling larvae of potentially zoonotic nematodes, this being the first report on A. cantonensis L₁.

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“…However, recently, a new approach to their use has been described. Braga et al (2011a) showed that the crude proteolytic extract of Duddingtonia flagrans (AC001), a predatory fungus, was effective in destroying A. vasorum L 1 . On the other hand, the predator nematophagous fungus Monacrosporium thaumasium has been used successfully in combating gastrointestinal nematodiosis under laboratory and natural conditions (Araújo et al 2008;Braga et al 2009).…”

Section: Introductionmentioning

confidence: 99%

In vitro activity of a serine protease from Monacrosporium thaumasium fungus against first-stage larvae of Angiostrongylus vasorum

et al. 2012

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A serine protease from the nematophagous fungus Monacrosporium thaumasium (NF34a) was purified, partially characterized and tested in vitro in control of the first larval stage of Angiostrongylus vasorum. NF34a grew in liquid culture medium, producing its crude extract that was purified by ion exchange chromatography. The fractions with high protease activity were collected in a pool, and elution of proteases was monitored by enzymatic assay and protein content. Purification steps were monitored by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Protease activity was determined under different pH and temperature conditions, and the inhibitor effects of metal ions and phenylmethylsulfonyl fluoride (PMSF) were assessed. In an experimental test, the infection process of NF34a on first-stage larvae of A. vasorum was investigated. A purified serine protease (Mt1) was identified, with an approximate molecular mass of 40 kDa and apparent homogeneity in SDS-PAGE, having optimal activity at pH 7.0 to 8.0 and temperature of 60°C. Mg(2+) and Zn(2+) partially inhibited the activity of Mt1 while PMSF inhibited it completely. Mt1 production was observed when NF34a was grown using first-stage larvae of A. vasorum as the only source of carbon and nitrogen. These results show that the enzyme may have a possible role in the infection process of the larvae. In the in vitro test of applicability against A. vasorum L(1), we observed a reduction in the number of larvae of 23.9% (p < 0.05) in the group treated with Mt1 compared with the control group. However, even this low reduction demonstrates that the Mt1 is important in the infection process.

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Atividade larvicida do extrato bruto enzimático do fungo Duddingtonia flagras sobre larvas de primeiro estádio de Angiostrongylus vasorum

Abstract: RESUMO

Cited by 10 publications

References 11 publications

Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures

Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures

First report of the activity of predatory fungi on Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) first-stage larvae

In vitro activity of a serine protease from Monacrosporium thaumasium fungus against first-stage larvae of Angiostrongylus vasorum

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