Identificação de espécies de Leishmania isoladas de casos humanos em Mato Grosso do Sul por meio da reação em cadeia da polimerase

Lima, Manoel Sebastião da Costa; Andreotti, Renato; Dorval, Maria Elizabeth Moraes Cavalheiros; Oshiro, Elisa Teruya; Oliveira, Alessandra Gutierrez de; Matos, Maria de Fátima Cepa

doi:10.1590/s0037-86822009000300012

Cited by 20 publications

(16 citation statements)

References 43 publications

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“…16,17,18,19 However, the test has limited diagnostic utility in endemic areas, as positive results may be obtained in patients without active lesions or with cicatricial lesions, suggesting the presence of subclinical infection or of other coexisting conditions. 7,17 In the present sample, early exposure to the parasite induced a positive immunological response in most individuals. In many cases, this is associated with spontaneous healing, which would explain the positive association between patient age, Montenegro test positivity, the degree of granulomatous transformation, and the number of epithelial cells, as well as the negative relationship between age and serology, since antibody titers were inversely proportional to effective cell response.…”

Section: Discussionmentioning

confidence: 56%

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American tegumentary leishmaniasis: correlations among immunological, histopathological and clinical parameters

Martins

Barreto

Lauris

et al. 2014

An. Bras. Dermatol.

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BACKGROUNDAmerican tegumentary leishmaniasis has an annual incidence of 1 to 1.5 million cases. In some cases, the patient's immune response can eliminate the parasite, and the lesion spontaneously resolves. However, when this does not occur, patients develop the disseminated form of the disease. OBJECTIVETo investigate the association between clinical, laboratory and pathological findings in cases of American tegumentary leishmaniasis. METHODSA retrospective study of the medical records of 47 patients with American cutaneous leishmaniasis. Clinical, laboratory and epidemiological data were collected, and semi-quantitative histopathological analyses were performed using the Spearman correlation coefficient (p <0.05). RESULTSMean patient age was 40.5 years. A total of 29.7% individuals were female and 70.2% were male, and 40.4% of the patients were farmers. The ulcerative form was found in 53.2% of patients, of whom 59.6% had lesions in the limbs. The average time to diagnosis was 22.3 months. The following positive correlations were significant: age and duration of the disease, Montenegro reaction, degree of granulomatous transformation and epithelioid cell count; duration of disease, Montenegro reaction and number of lymphocytes; epithelial hyperplasia and edema, hemorrhaging, and epithelial aggression; number of plasmocytes and number of parasites. The main negative correlations found were as follows: age and serology; time and parasite load; epithelial hyperplasia and degree of granulomatous transformation. CONCLUSIONThe long duration of the disease could be explained by the fact that lesions were relatively asymptomatic, and therefore ignored by patients with low literacy levels. Individuals may have simply waited for spontaneous healing, which proved to be dependent on the activation of hypersensitivity mechanisms.

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Section: Discussionmentioning

confidence: 56%

“…Indirect immunological methods such as the Montenegro intradermal reaction and indirect immunofluorescence serology may also help with diagnostic confirmation. 7 …”

Section: Introductionmentioning

confidence: 99%

American tegumentary leishmaniasis: correlations among immunological, histopathological and clinical parameters

Martins

Barreto

Lauris

et al. 2014

An. Bras. Dermatol.

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“…With these primers, [36] achieved a 100% level of positive results in peripheral blood samples from infected dogs, a contrasting finding that can be ascribed to differences in the parasitemia levels in dogs and humans [13,14,37,38] . The RV1/RV2 primers allow for the detection of less than one parasite per sample but do not amplify L. amazonensis and L. braziliensis, the causative agents of a number of cases of leishmaniasis in Brazil in general [17] and in Mato Grosso do Sul specifically [16,39,40] .…”

Section: Discussionmentioning

confidence: 99%

“…The RV1 and RV2 primers have been successfully used to identify Leishmania species, and to detect Leishmania (Leishmania) chagasi in dogs and phlebotomines [16][17][18][19] , as well as in human blood and urine [20,21] .…”

Section: P E E R R E V I E W Abstract Abstractmentioning

confidence: 99%

Sensitivity of PCR and real-time PCR for the diagnosis of human visceral leishmaniasis using peripheral blood

Lima

Zorzenon

Dorval

et al. 2013

Asian Pacific Journal of Tropical Disease

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“…This is particularly true in areas where Leishmania amazonensis could be present, such as Argentina (Frank et al 2003), as well as MS (Lima Junior et al 2009), where it could be infecting Lu. longipalpis (Savani et al 2009), a species that is also capable of transmitting VL (Almeida et al 1996, Aleixo et al 2006, Tolezano et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

Acardi

Liotta

Santini

et al. 2010

Mem. Inst. Oswaldo Cruz

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In this study, a genotypification of Leishmaniawas performed using polimerase chain reaction-restriction fragment length polymorfism (PCR-RFLP) and sequencing techniques to identify species of Leishmaniaparasites in phlebotomine sand flies and dogs naturally infected. Between January-February of 2009, CDC light traps were used to collect insect samples from 13 capture sites in the municipality of Posadas, which is located in the province of Misiones of Argentina. Sand flies identified as Lutzomyia longipalpiswere grouped into 28 separate pools for molecular biological analysis. Canine samples were taken from lymph node aspirates of two symptomatic stray animals that had been positively diagnosed with canine visceral leishmaniasis. One vector pool of 10 sand flies (1 out of the 28 pools tested) and both of the canine samples tested positively for Leishmania infantumby PCR and RFLP analysis. PCR products were confirmed by sequencing and showed a maximum identity with L. infantum. Given that infection was detected in one out of the 28 pools and that at least one infected insect was infected, it was possible to infer an infection rate at least of 0.47% for Lu. longipalpisamong the analyzed samples. These results contribute to incriminate Lu. longipalpis as the vector of L. infantumin the municipality of Posadas, where cases of the disease in humans and dogs have been reported since 2005

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Identificação de espécies de Leishmania isoladas de casos humanos em Mato Grosso do Sul por meio da reação em cadeia da polimerase

Cited by 20 publications

References 43 publications

American tegumentary leishmaniasis: correlations among immunological, histopathological and clinical parameters

American tegumentary leishmaniasis: correlations among immunological, histopathological and clinical parameters

Sensitivity of PCR and real-time PCR for the diagnosis of human visceral leishmaniasis using peripheral blood

Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

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