2006
DOI: 10.1590/s0037-86822006000600007
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Abstract: RESUMOOs vírus linfotrópicos de células T humanas, quando integrados ao genoma da célula hospedeira, provírus, têm como marcador de replicação seu DNA proviral. A carga proviral parece ser um importante fator no desenvolvimento de patologias associadas a estes retrovírus. Neste estudo foi desenvolvida uma metodologia para quantificação absoluta da carga proviral dos HTLV-1 e HTLV-2 através da PCR em tempo real. Cinqüenta e três amostras de doadores de sangue com teste de ELISA reagente foram submetidas à metod… Show more

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Cited by 30 publications
(16 citation statements)
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“…In our study, the presence of HTLV-1/2 was not detected in patients presenting at a tropical/infectious dermatology clinic, which is in contrast to previous reports of associations between HTLV-1/2 and skin diseases 13 . However, it is diffi cult to compare results obtained by studies with distinct study methodologies and on different population samples.…”
contrasting
confidence: 99%
See 1 more Smart Citation
“…In our study, the presence of HTLV-1/2 was not detected in patients presenting at a tropical/infectious dermatology clinic, which is in contrast to previous reports of associations between HTLV-1/2 and skin diseases 13 . However, it is diffi cult to compare results obtained by studies with distinct study methodologies and on different population samples.…”
contrasting
confidence: 99%
“…13 . A questionnaire was administered at the time of enrollment in the study and included questions about gender, age, skin color, marital status, and place of origin.…”
mentioning
confidence: 99%
“…Foram utilizadas três sequências alvo: as regiões não homólogas do gene pol do HTLV-1 e HTLV-2, e o gene da albumina como controle endógeno da reação. As sequências dos primers e sondas contidos nos ensaios, assim como o protocolo utilizado, foram descritos segundo Tamegão-Lopes et al 42 .…”
Section: Testes Sorológicosunclassified
“…The primers and probes were as previously described 24 . DNA (5μml) was used in the HTLV-1 reaction, presenting a final volume of 25μl consisting of 12.5μl of TaqMan PCR Master Mix (Applied Biosystems, CA, USA), 250nM of HTLV-1F (5'-GAACGCTCTAATGGCATTCTTAAAACC-3'), 250nM of HTLV-1R (5' -GTGGTTGATTGTCCATAGGGCTAT-3') and 200nM of HTLV-1P (5'-FAM-ACAAACCCGACCTACCC-MGB-3') for HTLV-1 pol region amplification; and 150nM of ALB-F (5'-GCTCAACTCCCTATTGCTATCACA-3'), 150nM of ALB-R (5'-GGGCATGACAGGTTTTGCAATATTA-3') and 100nM of ALB-P (5'-VIC-TTGTGGGCTGTAATCAT-MGB-3') for human albumin gene amplification.…”
Section: Real-time Pcr For Htlv-1 Andmentioning
confidence: 99%