2006
DOI: 10.1590/s0037-86822006000300002
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Abstract: Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains. Key-words: Coccidioides immitis. Long-term preservation. PCR. RESUMO

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Cited by 6 publications
(8 citation statements)
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“…In another study, Bezerra et al (2006) obtained 100% positivity analyzing the DNA of 19 cultures of C. immitis : twelve clinical isolates from the state of Piauí and seven isolates preserved for 50-75 years in the culture collection of the Department of Mycology from the Instituto Oswaldo Cruz at FIOCRUZ in Rio de Janeiro [19]. …”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In another study, Bezerra et al (2006) obtained 100% positivity analyzing the DNA of 19 cultures of C. immitis : twelve clinical isolates from the state of Piauí and seven isolates preserved for 50-75 years in the culture collection of the Department of Mycology from the Instituto Oswaldo Cruz at FIOCRUZ in Rio de Janeiro [19]. …”
Section: Discussionmentioning
confidence: 99%
“…was included in this study. From the Fungal Culture Collection at IOC/FIOCRUZ, six were identified as C. immitis (USA) and two as C. posadasii (Argentina); thirteen (nine clinical and four environmental) isolates identified as C. posadasii from Piauí/Brazil were preserved at the Laboratory of Mycology at IPEC/FIOCRUZ [19]. …”
Section: Methodsmentioning
confidence: 99%
“…The low viability of the isolates may be related to the preservation and storage conditions. The mineral oil method is based on the reduction of the metabolic rate of the microorganism due to decreased oxygen consumption, which possibly affects conidiogenesis and viability, especially in fungi such as dimorphic fungi which have stringent metabolism 1 . High rates of viability using this preservation method have mainly been reported for filamentous fungi; this is probably because dimorphic fungi are more susceptible to the accumulation of toxic metabolites that may occur during the process of metabolic rate reduction, causing a decrease in viable fungus recovery 22 .…”
Section: Discussionmentioning
confidence: 99%
“…However, no single method can be applied generically to all fungi. For instance, although both sterile water and mineral oil can be used for preservation of filamentous and dimorphic fungi for several years 25 , factors such as metabolism, age of the inoculums and quality of the mineral oil influence the ability of the fungus to survive under mineral oil 1,20 . However, as Coccidioides spp.…”
Section: Introductionmentioning
confidence: 99%
“…The first autochthonous cases of coccidioidomycosis in Brazil were reported in 1978 and 1979, the first case being from the State of Bahia 54 and the second one from Piauí. About 15 years later, the first micro‐outbreak of this mycosis in Brazil was also reported in the State of Piauí 55 . Since then, the number of published cases has increased considerably.…”
Section: Coccidioidomycosis In Latin Americamentioning
confidence: 99%