Comparison of the thick smear and Kato-Katz techniques for diagnosis of intestinal helminth infections

Santos, Fred Luciano Neves; Cerqueira, Elder Sânzio Aguiar; Soares, Neci Matos

doi:10.1590/s0037-86822005000200016

Cited by 55 publications

(45 citation statements)

References 3 publications

(3 reference statements)

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“…For example, the Kato-Katz technique increases the detection rates of some helminth species and provides quantitative results; however, the diagnosis of hookworm is hampered by the deterioration of these eggs in a short period of time, differentiation between hookworm species is not possible, and the detection S. stercoralis larvae is poor. 26,[29][30][31] These diagnostic limitations can be avoided with real-time PCR because it allows simultaneous detection and quantification of different targets and has been shown to be highly sensitive and specific for detection of many intestinal parasites. 9,[12][13][14][15][32][33][34][35][36][37] In this study, a pentaplex real-time PCR was evaluated to detect infections with the human roundworm, two species of hookworm and threadworm.…”

mentioning

confidence: 99%

A Pentaplex Real-Time Polymerase Chain Reaction Assay for Detection of Four Species of Soil-Transmitted Helminths

Basuni

Muhi²,

Othman³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

158

151

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mentioning

confidence: 99%

A Pentaplex Real-Time Polymerase Chain Reaction Assay for Detection of Four Species of Soil-Transmitted Helminths

Basuni

Muhi²,

Othman³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

158

151

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“…Methods in which the techniques densifying the developmental forms of parasites are not applied are, among others, the Stoll method, Caldwell method [17,18] and KatoKatz method [19][20][21].…”

Section: Basic Division Of Diagnostic Techniquesmentioning

confidence: 99%

“…This method serves the detection of helminths' eggs and larvae of worms (cellophane as cover strips soaked in glycerol-malachite green or methylene blue solution). The advantage of this method is the examination of a larger amount of faeces in one specimen, which increases the probability of the detection of parasites [19,20,37].…”

Section: Staining Of Specimensmentioning

confidence: 99%

Division of methods for counting helminths’ eggs and the problem of efficiency of these methods

Jaromin-Gleń

Kłapeć

Łagód

et al. 2017

Ann Agric Environ Med.

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From the sanitary and epidemiological aspects, information concerning the developmental forms of intestinal parasites, especially the eggs of helminths present in our environment in: water, soil, sandpits, sewage sludge, crops watered with wastewater are very important. The methods described in the relevant literature may be classified in various ways, primarily according to the methodology of the preparation of samples from environmental matrices prepared for analysis, and the sole methods of counting and chambers/instruments used for this purpose. In addition, there is a possibility to perform the classification of the research methods analyzed from the aspect of the method and time of identification of the individuals counted, or the necessity for staining them. Standard methods for identification of helminths' eggs from environmental matrices are usually characterized by low efficiency, i.e. from 30% to approximately 80%. The efficiency of the method applied may be measured in a dual way, either by using the method of internal standard or the 'Split/Spike' method. While measuring simultaneously in an examined object the efficiency of the method and the number of eggs, the 'actual' number of eggs may be calculated by multiplying the obtained value of the discovered eggs of helminths by inverse efficiency.

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“…However, the usefulness of these techniques may be limited because of their timeconsuming nature, labour intensiveness, and low sensitivity. The Kato-Katz quantitative technique, recommended by the World Health Organization (WHO), is suitable for the detection of geohelminth ova [7,106] and is most commonly implemented in human helminth surveys. However, the usefulness of the Kato-Katz method in detecting infections in infants may be limited, because stools of breastfeeding infants tend to be more liquid and have relatively low egg counts.…”

Section: Laboratorymentioning

confidence: 99%

Geohelminths: public health significance

Ojha

Jaide

Jinawath

et al. 2014

J Infect Dev Ctries

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The worldwide prevalence of geohelminths and their unique place in evolutionary biology have attracted research focus. These major soiltransmitted intestinal nematodes that cause human diseases are the nematode roundworm (Ascaris lumbricoides), the whipworm (Trichuris trichiura) and the two hookworms (Ancylostoma duodenale and Necator americanus), often collectively referred as geohelminths. Studies of geohelminthiasis in poorly nourished children in developing regions report that geohelminths contribute to stunted growth and cognitive impairment. Insights into immunology have shed light on the modulatory role of the parasite on the host immune system and have defined the role of T cells in controlling geohelminthic infection. Recent molecular biological techniques have created an opportunity to analyse the interaction between parasites and their hosts at the molecular level. This paper is a review of the recent literature that examined the prevalence of geohelminthiasis in developing countries, the association between geohelminths in relation to public health, parasitological/diagnostic features, and therapeutic and preventive aspects of these major soil-transmitted helminth (STH) pathogens in humans.

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Comparison of the thick smear and Kato-Katz techniques for diagnosis of intestinal helminth infections

Abstract: ABSTRACT

Cited by 55 publications

References 3 publications

A Pentaplex Real-Time Polymerase Chain Reaction Assay for Detection of Four Species of Soil-Transmitted Helminths

A Pentaplex Real-Time Polymerase Chain Reaction Assay for Detection of Four Species of Soil-Transmitted Helminths

Division of methods for counting helminths’ eggs and the problem of efficiency of these methods

Geohelminths: public health significance

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