Estudo comparativo de técnicas parasitológicas: Kato-Katz e coprotest®

Mendes, Célia Regina Pessanha; Teixeira, Angela Terezinha Lauand Sampaio; Pereira, R. M. R.; Dias, Lucas D.

doi:10.1590/s0037-86822005000200010

Cited by 23 publications

(21 citation statements)

References 2 publications

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“…Mendes e cols afirmam que, como rotina em laboratório, utiliza-se mais de um método coproscópico para a detecção de formas parasitárias de protozoários e helmintos, principalmente em amostras com baixa carga parasitária. A diferença de resultados entre as duas técnicas demonstra que um indivíduo não deve ser classificado como sadio pelo resultado negativo de um método isolado 22 .…”

Section: Resultados Discussãounclassified

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Palmeira

Carvalho

Rodrigues

et al. 2010

Rev. Soc. Bras. Med. Trop.

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RESUMO ABSTRACTIntroduction: Preliminary studies indicate a probable schistosomiasis expansion in Alagoas. This work analyzes the occurrence of Schistosoma mansoni in students from two municipalities, located in the Mundaú and Paraíba rivers basins. Methods: The study group consisted of 690 students in the urban area, aged between seven and 15 years. A standardized socio-economic questionnaire was filled out and parasitological tests were used to diagnosis schistosomiasis (Lutz and Kato methods). Positive cases were treated and students and their families were lectured about health education. Results: The Schistosoma mansoni prevalence was 24.9%. The association between schistosomiasis and other parasitic diseases was significant in relation to trichuriasis (p <0.05). Only 50% of infected children lived in brick homes with sanitation and public water supply. Among them, 48.1% used to go usually to the water collections and 55.8% of them knew rivers snails. The concordance analysis between the Lutz and Kato methods showed disagreement in 54.2%, with greater sensitivity for the Lutz technique (76.4%). Conclusions: The indices obtained characterize the municipalities as hyperendemic areas, because prevalence rates over 5% are considered high according to the World Health Organization. The high prevalence observed here is also two times higher than that reported by the Brazilian Schistosomiasis Program Control.

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Section: Resultados Discussãounclassified

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Palmeira

Carvalho

Rodrigues

et al. 2010

Rev. Soc. Bras. Med. Trop.

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“…It can also be used in epidemiological surveys as a guideline for the adoption of local prophylactic measures, and as an indication of the quality of population health. Therefore, this test is an extremely important component of human and veterinary medicine practices (VAZ, 2001;MENDES et al, 2005;TOSATO et al, 2005;MACHADO et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the accuracy of parasitological techniques for the diagnosis of intestinal parasites in cats

Rezende

Avelar

Storchilo

et al. 2015

Rev. Bras. Parasitol. Vet.

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The accuracy of the parasitological techniques of Willis, Hoffman-Pons-Janer or Lutz (HPLJ), Sheather and Faust was evaluated in fecal samples from stray cats caught by the Zoonosis Control Center in Goiânia, Goiás, Brazil. These four techniques were applied separately to analyze 154 fecal samples, and their accuracy was analyzed based on an evaluation of their sensitivity, specificity, positive and negative predictive values, and Kappa index, resulting in the selection of the Willis technique as the nominal gold standard. Of the 154 samples, 115 (74.68%) tested positive for intestinal parasites. The analysis of the frequency of positivity indicated that the HPLJ technique detected 86.1% of the positive samples and was the closest to the gold standard. The analysis of the accuracy of the techniques was evaluated using the most prevalent parasites. The Sheather technique showed the highest accuracy in the detection of Ancylostomatidae, while the Sheather and HPLJ techniques showed similar accuracies in the detection of Cystoisospora spp. when compared to the gold standard. Lastly, the Faust technique showed the highest accuracy in the detection of Toxoplasma gondii when compared to the gold standard. This study underscores the importance of combining parasitological techniques in the diagnosis of intestinal parasites in cats.Keywords: Accuracy, intestinal parasites, parasitological techniques, stray cats. ResumoA avaliação da acurácia das técnicas parasitológicas de Willis, Hoffman-Pons-Janer ou Lutz (HPJL), Sheather e Faust foram realizadas em amostras fecais de gatos capturados pelo Centro de Controle de Zoonoses em Goiânia, Goiás. Essas quatro técnicas foram utilizadas separadamente para análise de 154 amostras fecais. A análise de acurácia foi empregada, avaliando-se a sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e índice kappa, sendo a técnica de Willis eleita como padrão-ouro. Das amostras analisadas 74,68% (115/154) foram positivas para enteroparasitos. A análise de frequência de positividade demonstrou que a técnica HPJL detectou 86,1% dos positivos, aproximando-se do padrão-ouro. A avaliação da acurácia foi realizada pela análise dos parasitos mais prevalentes, sendo que, para Ancilostomídeos, a técnica de Sheather apresentou melhor acurácia; para Cystoisospora spp., as técnicas de Sheather e HPJL foram semelhantes e para Toxoplasma gondii, a técnica de Faust apresentou melhor acurácia comparada ao padrão ouro. Este estudo demonstra a importância da associação de técnicas parasitológicas para o diagnóstico de enteroparasitos em gatos.Palavras-chave: Acurácia, enteroparasitos, técnicas parasitológicas, gatos errantes.

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“…According to several researchers, coproscopy methods have different diagnostic efficacy due to the different composition and specific gravity of the flotant, the settling period for which helminth eggs should float, and the specific gravity of parasite eggs themselves (Kotelnikov, 1974;Mendes et al, 2005;Dakhno & Dakhno, 2010). Therefore, well-known flotation research methods for geese nematodes were tested.…”

Section: Resultsmentioning

confidence: 99%

“…Some have a destructive effect on the eggs of parasites, changing their characteristic morphological features. With the use of others, along with invasive elements, a large number of feed residues float to the surface, which also reduces their diagnostic efficiency (Nonaka et al, 1991;Mendes et al, 2005;Dahno & Dahno, 2010).…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of life-long diagnostic methods for geese nematodes

Pryima

2019

Ukr. Jour. of Vet. and Agr. Sci.

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The basis of successful struggle and specific prevention of poultry worms is timely diagnosis, the final stage of which is the detection of the worms themselves, their eggs or larvae at various stages of development. Priority is given to the methods of lifelong laboratory diagnosis of helminthiasis, which are preferably recommended for use in all animal species, including poultry. The aim of this work was to determine the sensitivity of flotation methods of coprovoscopy for geese nematodes. Experimental determination of the efficiency of the well-known methods of flotation and their comparative evaluation of coproovoscopic diagnosis of heterocosis, capillary disease and trichostrongilosis of geese. The main indicator of the diagnostic effectiveness of the methods was the intensity of the invasion and the time spent on the flotation of the samples. The most effective methods for diagnosing geese geoccosis are Kotelnikov-Hrenov (with ammonium nitrate) – at exposures of 20 min and Mallory (with saturated sugar solution) – at exposures of 10–15 min. The rates of invasion intensity were respectively 62.0 ± 4.39 and 59.0 ± 3.47 eggs/g. In the laboratory diagnosis of goose capillary disease, the most sensitive methods were Kotelnikov-Hrenov and Mallory at exposures of 15–20 min, where infestation rates reached 34.0 ± 2.22 and 33.5 ± 2.64 eggs/g, respectively. For trichostrongilosis, the Kotelnikov-Hrenov method showed the highest sensitivity at the exposure of 20 min, the intensity of the invasion was 32.5 ± 3.23 eggs/g. The Mallory method proved to be less effective – at an exposure of 15 min poultry invasiveness was 23.5 ± 1.81 eggs/g. The least sensitive of this invasion was the Fulleborn method (with NaCl), where the intensity of the invasion gradually increased with prolonged exposure and ranged from 10.5 ± 0.5 to 19.5 ± 2.45 eggs/g. Based on the data obtained, it is recommended to use the most sensitive methods and to take into account the exposure, which ensures the concentration of the largest number of nematode eggs on the surface of the flotant when conducting life-long coproovoscopic diagnostics of heterosis, capillary disease and trichostrongilosis of geese.

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Estudo comparativo de técnicas parasitológicas: Kato-Katz e coprotest®

Abstract: RESUMO

Cited by 23 publications

References 2 publications

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Evaluation of the accuracy of parasitological techniques for the diagnosis of intestinal parasites in cats

Sensitivity of life-long diagnostic methods for geese nematodes

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