Experimental schistosomiasis in the Common Marmoset Callithrix jacchus

Oliveira, Ana de; Malagueño, Elizabeth; Telles, Adriana Maria da Silva; Madruga, Maria Helena; Santana, José Carlos Curvelo

doi:10.1590/s0037-86822004000300006

Cited by 2 publications

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“…The inability to maintain S. stercoralis of human origin in the laboratory and the nearly complete absence of viable models for the study of auto-infection, hyperinfection and dissemination of this parasite have hampered our understanding of various aspects related to the biology and pathophysiology of this parasite. Marmosets are susceptible to infections with a number of parasites of medical importance [64][65][66][67][68][69][70], and the use of these animals has advantages over other non-human primates: smaller size (i.e., weight of only approximately 400 g), greater abundance of some species in nature, ease of captive breeding and handling, reduced maintenance costs and a relatively lower risk for the transmission of zoonoses to humans [56,64,71,72]. In this study, specimens of C. penicillata were susceptible to infection with S. stercoralis and complicated and uncomplicated disease occurred, confirming that these primates are a promising model for studying strongyloidiasis [56].…”

Section: Discussionmentioning

confidence: 99%

Strongyloides stercoralis infection in marmosets: replication of complicated and uncomplicated human disease and parasite biology

Mati

Raso²,

Melo

2014

Parasites & Vectors

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Both complicated and uncomplicated strongyloidiasis occur in C. penicillata that is described as a susceptible small non-human primate model for S. stercoralis. This host permits the maintenance of a human strain of the parasite in the laboratory and can be useful for experimental investigations of strongyloidiasis. In parallel, we discuss data related to the autoinfective cycle that provides new insights into the biology of S. stercoralis.

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Section: Discussionmentioning

confidence: 99%

Strongyloides stercoralis infection in marmosets: replication of complicated and uncomplicated human disease and parasite biology

Mati

Raso²,

Melo

2014

Parasites & Vectors

View full text Add to dashboard Cite

show abstract

Strongyloides stercoralis infection in marmosets: replication of complicated and uncomplicated human disease and parasite biology

Mati

Raso²,

Melo

2014

Parasites Vectors

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BackgroundStrongyloides stercoralis can undergo an alternative autoinfective life cycle in the host, which, in some individuals can lead to a lethal infection. However, due to a number of factors, such as, the majority of those infected are from low-income backgrounds and the limitation in experimental models for studying human S. stercoralis, strongyloidiasis remains neglected. Improved knowledge of animal models that are susceptible to this parasite is needed in order to investigate the immunological mechanisms involved during infection and in particular to further understand the natural history of the autoinfective cycle.MethodsCallithrix penicillata were inoculated subcutaneously with 100 (n = 2), 300 (n = 4) or 500 (n = 9) third-stage infective larvae (L3i) of S. stercoralis of human origin. Three marmosets received smaller inocula (i.e., one received 100 and two received 300 L3i) to ensure a greater capacity to withstand the infection after immunosuppression, which was triggered by administration of dexamethasone during early patency. Qualitative faecal analyses began at 7 days post-infection (DPI), and semi-quantitative tests were also performed for the dexamethasone-treated primates and the three matched controls. During the necropsies, specimens of S. stercoralis were recovered and tissue fragments were processed for histopathology.ResultsThe mean prepatency and patency periods were 16.1 ± 3.0 and 161.1 ± 72.2 DPI, respectively. The marmosets typically tolerated the infection well, but immunosuppressed individuals exhibited higher numbers of larvae in the faeces and progressive clinical deterioration with late disseminated infection. In these cases, the number of females recovered was significantly higher than the number of inoculated L3i. Large quantities of larvae were observed migrating through the host tissues, and histopathology revealed pulmonary and intestinal injuries consistent with those observed in human strongyloidiasis.ConclusionsBoth complicated and uncomplicated strongyloidiasis occur in C. penicillata that is described as a susceptible small non-human primate model for S. stercoralis. This host permits the maintenance of a human strain of the parasite in the laboratory and can be useful for experimental investigations of strongyloidiasis. In parallel, we discuss data related to the autoinfective cycle that provides new insights into the biology of S. stercoralis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-014-0579-2) contains supplementary material, which is available to authorized users.

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Experimental schistosomiasis in the Common Marmoset Callithrix jacchus

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Cited by 2 publications

References 30 publications

Strongyloides stercoralis infection in marmosets: replication of complicated and uncomplicated human disease and parasite biology

Strongyloides stercoralis infection in marmosets: replication of complicated and uncomplicated human disease and parasite biology

Strongyloides stercoralis infection in marmosets: replication of complicated and uncomplicated human disease and parasite biology

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