2003
DOI: 10.1590/s0037-86822003000400001
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Abstract: Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Signific… Show more

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Cited by 8 publications
(11 citation statements)
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“…Two antigens with these characteristics were used in this study: cytoplasmatic repetitive antigen (CRA), present in evolved forms of the epimastigote and amastigote of T. cruzi, and flagellar repetitive antigen (FRA), present in the epimastigote and trypomastigote forms of the parasite (9,10). The immune response to these antigens has already been studied in mice (11)(12)(13)(14), in their diagnostic potential and to monitor posttreatment cure (15)(16)(17). However, it is not known which isotypes of immunoglobulin G (IgG) are involved in the immune response generated in patients with Chagas' disease in the presence of these recombinant antigens (Ags-Recs).…”
Section: Introductionmentioning
confidence: 99%
“…Two antigens with these characteristics were used in this study: cytoplasmatic repetitive antigen (CRA), present in evolved forms of the epimastigote and amastigote of T. cruzi, and flagellar repetitive antigen (FRA), present in the epimastigote and trypomastigote forms of the parasite (9,10). The immune response to these antigens has already been studied in mice (11)(12)(13)(14), in their diagnostic potential and to monitor posttreatment cure (15)(16)(17). However, it is not known which isotypes of immunoglobulin G (IgG) are involved in the immune response generated in patients with Chagas' disease in the presence of these recombinant antigens (Ags-Recs).…”
Section: Introductionmentioning
confidence: 99%
“…They were further evaluated for any contamination with bacterial proteins and carbohydrates, as described by Pereira et al (8) Heparinized peripheral blood was cultured for about 24h and diluted 1:1 in 14mL polypropylene tubes (BD Biosciences, San Jose, CA, USA) containing RPMI 1640 medium supplemented with L-glutamine, 1% antibiotics (stock solution of 10,000U penicillin and 10,000U streptomycin; Sigma-Aldrich, St. Louis, MO, USA), and 10% fetal bovine serum (Sigma-Aldrich). Blood was stimulated as described by Lorena et al (9) .…”
mentioning
confidence: 99%
“…These antigens were able to elicit the production of specific IgG immunoglobulins. CRA induced predominantly the production of IgG1 and IgG3 isotypes in BALB/c and C57BL/6 mice, while FRA induced antibodies of the IgG1 isotype in BALB/c mice (Pereira et al 2003a(Pereira et al , 2003b. Cellular lymphoproliferative response was also observed in both mouse strains.…”
Section: Introductionmentioning
confidence: 88%
“…We have previously demonstrated the immunogenicity of cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) recombinant proteins of T. cruzi by evaluating the humoral and cellular responses in uninfected susceptible (BALB/c) and resistant (C57BL/6) mice strains immunized with these proteins (Pereira et al 2003a(Pereira et al , 2003b(Pereira et al , 2004. These antigens were able to elicit the production of specific IgG immunoglobulins.…”
Section: Introductionmentioning
confidence: 99%