Loose and Compact Agglomerates of 50 Nm Microvesicles Derived From Golgi and Endoplasmic Reticulum Membranes in Pre- And in -Apoptotic Mycoplasma Infected Hela Cells: Host-Parasite Interactions Under the Transmission Electron Microscope

Sesso, Antônio; Yamashiro-Kanashiro, Edite H.; Orii, Noemia Mie; Taniwaki, Noemi Nosomi; Kawakami, Joyce T.; Carneiro, Sylvia Mendes

doi:10.1590/s0036-46652015000100015

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“… 19 and Sesso et al . 16 and revealed undescribed structural components of the infected cells cytoplasmic dismantling. As for the novel findings, here we comment mainly the appearance of new bacteria on the host cell surface and the finding of the cells.…”

Section: Discussionmentioning

confidence: 99%

“…Some of these samples devoid of typical bacterial forms are under study to detect whether other(s) organism(s) is(are) infecting host cells. Preliminary results obtained in HeLa cells heavily infected with mycoplasma revealed marked alterations in the endoplasmic reticulum (ER) and Golgi apparatus membranes in apoptotic cells, but also in the non-apoptotic ones 16 . Expanding upon these initial observations, we observed in samples from most cell donor's mycoplasma inflicted cytoplasmic alterations compromising the cell periphery, as the appearance of villus-like elements with associated 80-200 nm diameter vesicles.…”

Section: Introductionmentioning

confidence: 99%

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Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles

Sesso

Yamashiro-Kanashiro

Arruda

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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A study with transmission electron microscopy of mycoplasma-contaminated HeLa cells using five cell donors referred to as donors A, B, C, D and E, observations are herein presented. Experiments performed with cells from donors B, C and D, revealed the presence of Mycoplasma hyorhinis after PCR and sequencing experiments. Bacteria probably originated from a cytoplasm with compacted tiny granular particles replacing the normal cytosol territories, or from the contact with the cytoplasm through a clear semi-solid material. The compact granularity (CG) of the cytoplasm was crossed by stripes of smooth and rough endoplasmic reticulum cisternae. Among apparently normal mitochondria, it was noted, in variable proportions, mitochondria with crista-delimited lucent central regions that expand to and occupied the interior of a crista-less organelle, which can undergo fission. Other components of the scenarios of mycoplasma-induced cell demolition are villus-like structures with associated 80-200 nm vesicles and a clear, flexible semi-solid, process-sensitive substance that we named jam-like material. This material coated the cytoplasmic surface, its recesses, irregular protrusions and detached cytoplasmic fragments. It also cushioned forming bacteria. Cyst-like structures were often present in the cytoplasm. Cells, mainly apoptotic, exhibiting ample cytoplasmic sectors with characteristic net-like profile due to adjoined vacuoles, as well as ovoid or elongated profiles, consistently appeared in all cells from the last four cell donors. These cells were named “modified host cells” because bacteria arose in the vacuoles. The possibility that, in some samples, there was infection and/or coinfection of the host cell by another organism(s) cannot be ruled out.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%