Preliminary study towards a novel experimental model to study localized cutaneous leishmaniasis caused bY Leishmania (Leishmania) mexicana

Sosa-Bibiano, Erika Ivett; Wynsberghe, Nicole R. Van; Canto-Lara, Silvia B.; Andrade-Narvaez, Fernando J.

doi:10.1590/s0036-46652012000300009

Cited by 11 publications

(10 citation statements)

References 34 publications

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“…yucatanicus has been adapted to captivity and a colony has been established for experimental studies in our institution. Previous studies have demonstrated that P. yucatanicus experimentally infected with 1 x 10 6 L. (L.) mexicana promastigotes showed clinical and histological features similar to those of humans with CL caused by the same parasite species (Sosa-Bibiano et al 2012). These results support the utility of P. yucatanicus as a novel experimental model to study CL caused by L. (L.) mexicana.…”

supporting

confidence: 71%

Nitric oxide production by Peromyscus yucatanicus (Rodentia) infected with Leishmania (Leishmania) mexicana

Loría-Cervera

Sosa-Bibiano

Villanueva‐Lizama

et al. 2013

Mem. Inst. Oswaldo Cruz

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supporting

confidence: 71%

Nitric oxide production by Peromyscus yucatanicus (Rodentia) infected with Leishmania (Leishmania) mexicana

Loría-Cervera

Sosa-Bibiano

Villanueva‐Lizama

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…It has been adapted to the laboratory and a colony was established for experimental studies. P. yucatanicus inoculated with 10 6 promastigotes of L. (L.) mexicana on the base of the tail reproduced both the clinical and histopathological picture of CL in humans, supporting its utility as a novel experimental model to study CL caused by L. (L.) mexicana 104 . Moreover 100% of P. yucatanicus inoculated with 10 2 (“low inoculum”) developed subclinical infection (absence of clinical signs and evidence of parasite's DNA at the site of inocula) and when immunosupressed with cyclophosphamide a reactivation with the appearance of lesions was observed 29 .…”

Section: Wild Rodentsmentioning

confidence: 79%

Animal Models for the Study of Leishmaniasis Immunology

Loría-Cervera

Andrade-Narvaez

2014

Rev. Inst. Med. trop. S. Paulo

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120

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Leishmaniasis remains a major public health problem worldwide and is classified as Category I by the TDR/WHO, mainly due to the absence of control. Many experimental models like rodents, dogs and monkeys have been developed, each with specific features, in order to characterize the immune response to Leishmania species, but none reproduces the pathology observed in human disease. Conflicting data may arise in part because different parasite strains or species are being examined, different tissue targets (mice footpad, ear, or base of tail) are being infected, and different numbers (“low” 1×102 and “high” 1×106) of metacyclic promastigotes have been inoculated. Recently, new approaches have been proposed to provide more meaningful data regarding the host response and pathogenesis that parallels human disease. The use of sand fly saliva and low numbers of parasites in experimental infections has led to mimic natural transmission and find new molecules and immune mechanisms which should be considered when designing vaccines and control strategies. Moreover, the use of wild rodents as experimental models has been proposed as a good alternative for studying the host-pathogen relationships and for testing candidate vaccines. To date, using natural reservoirs to study Leishmania infection has been challenging because immunologic reagents for use in wild rodents are lacking. This review discusses the principal immunological findings against Leishmania infection in different animal models highlighting the importance of using experimental conditions similar to natural transmission and reservoir species as experimental models to study the immunopathology of the disease.

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“…The recent model by Sosa-Bibiano et al, 2012, made use of local mammalian fauna, the Yucatan deer mouse ( Peromyscus yucatanicus ), as a model host to study the development of L . mexicana infection with the aim of mimicking natural infection conditions [ 54 ]. The typical human patient will develop a localized cutaneous nodular ulcerated lesion when infected by L .…”

Section: New World: L Mexicana mentioning

confidence: 99%

A Review: The Current In Vivo Models for the Discovery and Utility of New Anti-leishmanial Drugs Targeting Cutaneous Leishmaniasis

et al. 2015

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The current in vivo models for the utility and discovery of new potential anti-leishmanial drugs targeting Cutaneous Leishmaniasis (CL) differ vastly in their immunological responses to the disease and clinical presentation of symptoms. Animal models that show similarities to the human form of CL after infection with Leishmania should be more representative as to the effect of the parasite within a human. Thus, these models are used to evaluate the efficacy of new anti-leishmanial compounds before human clinical trials. Current animal models aim to investigate (i) host–parasite interactions, (ii) pathogenesis, (iii) biochemical changes/pathways, (iv) in vivo maintenance of parasites, and (v) clinical evaluation of drug candidates. This review focuses on the trends of infection observed between Leishmania parasites, the predictability of different strains, and the determination of parasite load. These factors were used to investigate the overall effectiveness of the current animal models. The main aim was to assess the efficacy and limitations of the various CL models and their potential for drug discovery and evaluation. In conclusion, we found that the following models are the most suitable for the assessment of anti-leishmanial drugs: L. major–C57BL/6 mice (or–vervet monkey, or–rhesus monkeys), L. tropica–CsS-16 mice, L. amazonensis–CBA mice, L. braziliensis–golden hamster (or–rhesus monkey). We also provide in-depth guidance for which models are not suitable for these investigations.

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Preliminary study towards a novel experimental model to study localized cutaneous leishmaniasis caused bY Leishmania (Leishmania) mexicana

Cited by 11 publications

References 34 publications

Nitric oxide production by Peromyscus yucatanicus (Rodentia) infected with Leishmania (Leishmania) mexicana

Nitric oxide production by Peromyscus yucatanicus (Rodentia) infected with Leishmania (Leishmania) mexicana

Animal Models for the Study of Leishmaniasis Immunology

A Review: The Current In Vivo Models for the Discovery and Utility of New Anti-leishmanial Drugs Targeting Cutaneous Leishmaniasis

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