Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis

Abstract

SUMMARYThe aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELISA using cyst wall, protoscolex and cyst fluid was 96.66%, 86.66% and 93.33% respectively and the specificity of the assay was 70% for Dot-ELISA using cyst fluid, protoscolex and cyst wall antigens. Results of the present study show that cyst wall and protoscolex can also be an useful source of antigen in detection of hydatid antibodies in the serodiagnosis of CE.

Increased Alkaline Phosphatase Activity in the Somatic Extract of Hydatid Cyst Protoscoleces upon Treatment with Albendazole, an Implication for in vitro Evaluation of Drug Efficiency

“…The samples were then sonicated in a 150w ultrasonic disintegrator (Institute of Biochemistry and Biophysics, Tehran, Iran) on ice 3-5 times (10 seconds follwed by 10 seconds rest) until no intact parasite were visible microscopically. The suspension was centrifuged at 10000 g for 30 min at 4°C, and the supernatant was stored at -20°C [11][12]. )…”

A review on diagnostic and preventive aspects of cystic echinococcosis and human cysticercosis

“…[4] Serological assays like indirect hemagglutination (IHA) and intradermal skin test (Casoni's test) were quite popular couple of decades back but are rarely performed now. [3233] Other antibody detection formats which have been evaluated are dot-ELISA, latex agglutination, etc. [3334] Siavashi et al .,[34] reported dot-ELISA to have 100% sensitivity and 98.7% specificity in diagnosing surgically proven cases of CE and found the performance to be better than a sandwich ELISA.…”

“…[3233] Other antibody detection formats which have been evaluated are dot-ELISA, latex agglutination, etc. [3334] Siavashi et al .,[34] reported dot-ELISA to have 100% sensitivity and 98.7% specificity in diagnosing surgically proven cases of CE and found the performance to be better than a sandwich ELISA.…”

“…[334041] Swarna et al .,[33] evaluated the diagnostic potential of different components of hydatid cyst like the cyst wall antigen, hydatid cyst fluid and protoscoleces in a DOT-ELISA format and found no significant difference in the performance of assay using any of these antigens. Some scientists have even evaluated the potential of adult E. granulosus antigen in immunodiagnostic assays.…”

A dot-ELISA using a partially purified cathepsin-L-like protein fraction fromTaenia soliumcysticerci, for the diagnosis of human neurocysticercosis

“…Unfortunately, EITB and standard ELISA require sophisticated equipment and trained personnel and are too complex to be used routinely under field conditions in developing countries. The simpler dot-ELISA, which have already been used to diagnose bacterial meningitis (Belo et al ., 2010), avian influenza virus infection (Chen et al ., 2008), toxocariasis (Roldán et al ., 2009), cystic echinococcosis (Swarna and Parija, 2008), fascioliasis (Shaheen et al ., 1989) and leishmaniasis (Pappas et al ., 1983), may offer an efficient immunodiagnostic method for NCC in resource-poor areas (Xu et al ., 1989). Such assays are relatively rapid and easy to perform, generally use components that are inexpensive and/or easily manufactured, and only require small amounts of antigen (Kondo et al ., 2007; Belo et al ., 2010).…”