2008
DOI: 10.1590/s0036-46652008000300004
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Abstract: SUMMARYHuman Immunodeficiency Vírus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and imunofluorescence was the definitive confirmatory test.The samples were obtained from the Hospital Nossa Senhora da Conceição in Porto Alegre, RS -Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul… Show more

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Cited by 35 publications
(40 citation statements)
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“…Patients self-collect small drops of blood onto a Whatman 903 protein saver card for mailing to the institute conducting the study. Such cards have been used by others to evaluate titers of antibodies to specific antigens (4,7,11,12). Since the immunosignature is a measure of total relative antibody reactivity, the utility of collecting dried-blood spots for immunosignature-based diagnostics is highly dependent on the ability to achieve near complete recovery of the antibody repertoire.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Patients self-collect small drops of blood onto a Whatman 903 protein saver card for mailing to the institute conducting the study. Such cards have been used by others to evaluate titers of antibodies to specific antigens (4,7,11,12). Since the immunosignature is a measure of total relative antibody reactivity, the utility of collecting dried-blood spots for immunosignature-based diagnostics is highly dependent on the ability to achieve near complete recovery of the antibody repertoire.…”
Section: Resultsmentioning
confidence: 99%
“…Others have previously demonstrated successful collection and retention of serum antibodies from whole-blood samples spotted on absorbent filter paper. In these studies, serum was evaluated by enzymelinked immunosorbent assay (ELISA) for antibodies to HIV (2,4,23), measles virus (6, 11-13, 24, 29), the malaria parasite, hepatitis C virus (3,5,15), and other pathogens (7,22,28). These studies demonstrated a good correlation between fresh plasma and driedblood samples using standard ELISA techniques, leading to the expectation that antibodies stored in dried-blood spots should be amenable to immunosignaturing.…”
mentioning
confidence: 99%
“…DBS have been used for the detection of therapeutic antibodies (12,16), and HIV (17,18), cytomegalovirus (19), hepatitis (20, 21), and herpes simplex antibodies (22). Several in-house and commercial ELISAs have been used to detect IgG against T. cruzi using serum and DBS (23)(24)(25)(26)(27)(28)(29)(30).…”
Section: Discussionmentioning
confidence: 99%
“…Similar to many other studies prior to this DBS collection and processing were performed by controlled application of EDTA blood onto filter paper in laboratory conditions instead of whole blood collected directly from heel or finger-prick in a real-world situation (Uttayamakul et al, 2005;Castro et al, 2008;Kane et al, 2008;Mbida et al, 2009). Although the method of DBS collection onto the circles on the filter paper is easy and requires minimal training, the final appearances of the blood spots on the filter papers would still require standardization.…”
Section: Discussionmentioning
confidence: 99%