2007
DOI: 10.1590/s0036-46652007000200012
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Abstract: SUMMARYWhooping cough or pertussis was a major cause of childhood morbidity and mortality in the world until the introduction of a whole-cell vaccine in the 1940's. However, since the early 1980's whooping cough cases have increased in many countries, becoming an important problem of public health. This increase may be due to accuracy of laboratory diagnosis and reporting of the disease, a decline in immunity over time, demographic changes, and adaptation of the bacterial population to vaccine-induced immunity… Show more

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Cited by 10 publications
(4 citation statements)
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“…Pulsed-field gel electrophoresis also found an outbreak and source of infection among B. pertussis recovered from Brazilian patients (33). Similarly, in Sweden, Advani.…”
Section: Discussionmentioning
confidence: 99%
“…Pulsed-field gel electrophoresis also found an outbreak and source of infection among B. pertussis recovered from Brazilian patients (33). Similarly, in Sweden, Advani.…”
Section: Discussionmentioning
confidence: 99%
“…Specimens were cultured on charcoal agar with 10% sterile defibrinated sheep blood and cephalexin and incubated at 35 to 37°C for 10 days. Colonies suggestive of belonging to the genus Bordetella were confirmed by Gram stain and biochemical tests, as previously described (10). Detection of Bordetella pertussis O1 antigen and serotyping were performed by slide agglutination using O1, Fim 2, and Fim 3 antibodies, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…The samples that showed growth from the third or fourth day, with colonies suggestive of belonging to the genus Bordetella were confirmed by Gram staining. The biochemical and antigenic characterizations were realized as described previously (19).…”
Section: Clinical Samples and Culturementioning
confidence: 99%