2004
DOI: 10.1590/s0036-46652004000500003
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Abstract: The aim of the present study was to standardize and evaluate dot-Enzyme linked immunosorbent assay (Dot-ELISA), a simple and rapid test for the detection of cysticercus antibodies in the serum for the diagnosis of neurocysticercosis (NCC). The antigen used in the study was a complete homogenate of Cysticercus cellulosae cysts obtained from infected pigs and dotted on to nitrocellulose membrane. Test sera were collected from the patients of NCC, and control sera from patients with other diseases and healthy stu… Show more

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Cited by 20 publications
(21 citation statements)
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“…Our study is more comparable to a study done by R.Biswas 15 showing a sensitivity of 43.75% and specificity of 98%. G Singh et al 12 have also shown a sensitivity of 48% with a similar commercially available ELISA kit as was used in our study.…”
Section: Serological Studysupporting
confidence: 85%
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“…Our study is more comparable to a study done by R.Biswas 15 showing a sensitivity of 43.75% and specificity of 98%. G Singh et al 12 have also shown a sensitivity of 48% with a similar commercially available ELISA kit as was used in our study.…”
Section: Serological Studysupporting
confidence: 85%
“…15 NCC has not been commonly reported from Mumbai (western) and Kolkata (eastern) regions. 16 Serum ELISA for IgG was positive in 27 cases showing a sensitivity of 48.22% and a specificity of 100%.…”
Section: Serological Studymentioning
confidence: 91%
“…Dot ELISA has become increasingly popular for the diagnosis of many other parasitic diseases also, such as fi lariasis, [8] malaria, [9] and schistosomiasis, [10] besides cysticercosis. Dot ELISA has been used earlier for diagnosis of cysticercosis using antigens derived from cysticercus cellulosae, [11][12][13] cysticercus longicollis, [14] and T. solium glycoproteins. [15] Liu et al, 1996, have modifi ed the Dot ELISA by Dot-immunogold silver staining for diagnosis of cysticercosis.…”
Section: Discussionmentioning
confidence: 99%
“…Both tests gave similar sensitivity, viz., 89%; while ELISA had better specifi city, viz., 81%, than Dot Blot (73%). [17] In another study by Biswas et al, Dot-ELISA was found to be 56.25% sensitive and 92% specifi c, as compared to plate ELISA, which was 43.75% sensitive and 98% specifi c. [12] Jiang et al have observed confi rmed cases of neurocysticercosis showing 81.6% to 96.1% positive reactions in Dot-ELISA. [11] It is reported that sensitivity and specifi city of Dot ELISA generally vary from 56.25% to 97.61% and from 90.6% to 92% respectively.…”
Section: Discussionmentioning
confidence: 99%
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