Molecular analysis of the dengue virus type 1 and 2 in Brazil based on sequences of the genomic envelope-nonstructural protein 1 junction region

Abstract

The genomic sequences of the Envelope-Non-Structural protein 1 junction region (E/NS1) of 84 DEN-1 and 22 DEN-2 isolates from Brazil were determined. Most of these strains were isolated in the period from 1995 to 2001 in endemic and regions of recent dengue transmission in São Paulo State. Sequence data for DEN-1 and DEN-2 utilized in phylogenetic and split decomposition analyses also include sequences deposited in GenBank from different regions of Brazil and of the world. Phylogenetic analyses were done using both maximum likelihood and Bayesian approaches. Results for both DEN-1 and DEN-2 data are ambiguous, and support for most tree bipartitions are generally poor, suggesting that E/NS1 region does not contain enough information for recovering phylogenetic relationships among DEN-1 and DEN-2 sequences used in this study. The network graph generated in the split decomposition analysis of DEN-1 does not show evidence of grouping sequences according to country, region and clades. While the network for DEN-2 also shows ambiguities among DEN-2 sequences, it suggests that Brazilian sequences may belong to distinct subtypes of genotype III.

Seroprevalence for dengue virus in a hyperendemic area and associated socioeconomic and demographic factors using a cross-sectional design and a geostatistical approach, state of São Paulo, Brazil

“…aegypti reinfestation in the state of São Paulo was detected in 1985 [54] and the first dengue epidemic occurred in 1990 and 1991, with the circulation of DENV-1 [21]. DENV-2, 3 and 4 were introduced respectively in 1998, 2002 [55] and 2011 [36]. Despite the infestation by the vector in Sao Paulo have been detected only in the 1980s and the first epidemic of dengue have occurred in the early 1990s, the state has had high incidences of dengue and deaths.…”

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

“…Subsequent DENV introductions occurred in 1997 and 2002, when DENV-2 and DENV-3 caused huge epidemics, with an increasing number of mild and severe cases 16,36,38 .…”

Coleção Entomológica de Referência: 85 anos de contribuições à saúde pública

“…Sallum’s scientific work includes taxonomic studies of diverse groups of Anophelinae and Culicinae, descriptions of new species, reviews, phylogenetic studies with morphological and molecular approach, including the characterization of the mitochondrial genome of multiple species of Anophelinae 29 , 30 and the genus Culex 31 . Other research approaches by Sallum and collaborators include aspects of the epidemiology and ecology of malaria, anopheline biology, ecology and behavior of culicids, list of species present in remote areas of the Brazilian Amazon, molecular characterization of the yellow fever virus 32 , dengue 33 and Saint Louis 34 , incrimination of Haemagogus leucocelaenus (Dyar, 1925) and Aedes serratus (Theobald, 1901) as vectors of the Yellow Fever virus in Rio Grande do Sul in 2008 35 . Investigations into environmental changes that include both degradation and disturbance of the Amazon rainforest and occurrence of malaria are important contributions, as they evidenced factors that act on vector mosquitoes and the occurrence of the disease.…”

“…A obra científica de Sallum inclui estudos taxonômicos de grupos diversos de Anophelinae e Culicinae, descrições de novas espécies, revisões, estudos filogenéticos com abordagem morfológica e molecular, incluindo a caraterização do genoma mitocondrial de múltiplas espécies de Anophelinae 29 , 30 e do gênero Culex 31 . Outras abordagens das pesquisas de Sallum e colaboradores incluem aspectos da epidemiologia e ecologia da malária, biologia de anofelíneos, ecologia e comportamento de culicídeos, lista de espécies presentes em áreas remotas da Amazônia brasileira, caracterização molecular do vírus da febre amarela 32 , dengue 33 e Saint Louis 34 , incriminação do Haemagogus leucocelaenus (Dyar, 1925) e Aedes serratus (Theobald, 1901) como vetores do vírus Febre Amarela no Rio Grande do Sul em 2008 35 . As investigações sobre alterações ambientais que incluem tanto a degradação como a perturbação da floresta Amazônica e ocorrência de malária são contribuições que merecem destaque, pois evidenciaram fatores que atuam nos mosquitos vetores e na ocorrência da doença.…”