Detection of Vibrio parahaemolyticus and Vibrio cholerae in oyster, Crassostrea rhizophorae, collected from a natural nursery in the Cocó river estuary, Fortaleza, Ceará, Brazil

Abstract

SUMMARYOysters are edible organisms that are often ingested partially cooked or even raw, presenting therefore a very high risk to the consumers' health, especially in tropical regions. The presence of Vibrio cholerae and Vibrio parahaemolyticus in oysters sampled at an estuary in the Brazilian northeastern region was studied, with 300 oysters tested through an 8-months period. The salinity of the water at the sampling point varied between 3% and 27‰. V. cholerae was the most frequently detected species (33.3% of the samples), and of the 22 V. cholerae isolates, 20 were identified as non-O1/non-O139, with two of the colonies presenting a rough surface and most of remaining ones belonging to the Heiberg II fermentation group. V. parahaemolyticus was isolated from just one of the samples. Other bacteria such as Providencia spp., Klebsiella spp. and Morganella morganii were also isolated.

Occurrence of Vibrio Pathotypes in the Final Effluents of Five Wastewater Treatment Plants in Amathole and Chris Hani District Municipalities in South Africa

“…Previous reports have articulated the isolation of Vibrio species from different niches and geographical regions. In marine environments of Italy Gugliandolo et al [35] found V. vulnificus as a dominant species, while Sousa et al [36] isolated V. parahaemolyticus and V. cholera in oysters collected in Brazil. Also Tuning et al .…”

Comparison of different biochemical and molecular methods for the identification of Vibrio parahaemolyticus

“…Vibrio parahaemolyticus is widely spread in estuarine and coastal marine environments and can be detected in various types of seafood throughout the world (DePaola et al 1990(DePaola et al , 2003Croci et al 2001;de Sousa et al 2004;Hayat Mahmud et al 2006). Food poisoning associated with this micro-organism is commonly reported, particularly from countries with both high seawater temperature and traditional consumption of raw or undercooked seafood (Potasman et al 2002;Martinez-Urtaza et al 2004).…”

Detection of environmental Vibrio parahaemolyticus using a polyclonal antibody by flow cytometry

“…Vibrio parahaemolyticus is a Gram‐negative halophilic bacterium found in estuarine waters worldwide and it is recognized as being a leading cause of food‐borne gastroenteritis (Potasman et al. , 2002; de Sousa et al. , 2004; Hayat Mahmud et al.…”