Paracoccidioides brasiliensis: A MYCOLOGIC AND IMMUNOCHEMICAL STUDY OF TWO STRAINS

Lacaz, Carlos da Silva; Vidal, Mônica Scarpelli Martinelli; Heins‐Vaccari, Elisabeth Maria; Melo, Natalina Takahashi de; Negro, G. Del; Arriagada, Giovana L. Hernández; Freitas, Roseli dos Santos

doi:10.1590/s0036-46651999000200004

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…Yeasts are multinucleated and of varying sizes (4 to 50 µm), and they have a spherical, oval, or elongated shape and multiple buds. When yeasts are cultured in solid medium, they present elevated colonies with irregular borders, cerebriform appearance, and cream coloring, becoming darkened with ageing [53].…”

Section: Fungal Biology 21 Dimorphic Transitionmentioning

confidence: 99%

Updates in Paracoccidioides Biology and Genetic Advances in Fungus Manipulation

Chaves

Navarro

Barros

et al. 2021

JoF

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The dimorphic fungi of the Paracoccidioides genus are the causative agents of paracoccidioidomycosis (PCM). This disease is endemic in Latin America and primarily affects workers in rural areas. PCM is considered a neglected disease, despite being a disabling disease that has a notable impact on the public health system. Paracoccidioides spp. are thermally dimorphic fungi that present infective mycelia at 25 °C and differentiate into pathogenic yeast forms at 37 °C. This transition involves a series of morphological, structural, and metabolic changes which are essential for their survival inside hosts. As a pathogen, the fungus is subjected to several varieties of stress conditions, including the host immune response, which involves the production of reactive nitrogen and oxygen species, thermal stress due to temperature changes during the transition, pH alterations within phagolysosomes, and hypoxia inside granulomas. Over the years, studies focusing on understanding the establishment and development of PCM have been conducted with several limitations due to the low effectiveness of strategies for the genetic manipulation of Paracoccidioides spp. This review describes the most relevant biological features of Paracoccidioides spp., including aspects of the phylogeny, ecology, stress response, infection, and evasion mechanisms of the fungus. We also discuss the genetic aspects and difficulties of fungal manipulation, and, finally, describe the advances in molecular biology that may be employed in molecular research on this fungus in the future.

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Section: Fungal Biology 21 Dimorphic Transitionmentioning

confidence: 99%

Updates in Paracoccidioides Biology and Genetic Advances in Fungus Manipulation

Chaves

Navarro

Barros

et al. 2021

JoF

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“…Na última avaliação, seis pacientes haviam morr i d o (46,1%), um não foi encontrado e seis foram avaliados de acordo com a escala de seguimento de Karn o f s k y, sendo dois (15,4%)com 100 e quatro (30 como Paracoccidioides brasiliensis (Fig 5). O term o blastomicose sul-americana não é apropriado, pois a moléstia não se restringe à América do Sul e pode levar a confusão na literatura mundial com a blastomicose (outra doença (Fig 6).…”

Section: Fig 3 Cortes Histológicos Demonstrando O P Brasiliensis Cunclassified

Paracoccidioidomicose do sistema nervoso central: análise de 13 casos

Fagundes-Pereyra

Carvalho²,

Góes

et al. 2006

Arq. Neuro-Psiquiatr.

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A paracoccidioidomicose (PCM) é infecção granulomatosa sistêmica, causada pelo fungo Paracoccidioides brasiliensis, prevalente na América Latina, particularmente no Brasil. Acomete o sistema nervoso central (SNC) em 10% dos casos. Foram estudados 13 pacientes com paracoccidioidomicose no SNC, entre 1991 e 2001, com ênfase para os aspectos clínicos, neuroradiológicos e terapêuticos. Onze pacientes eram do sexo masculino (84,6%) e dois do feminino (15,4%), com idade entre 30 e 71 anos (M= 47,1 ± 11,6 Me= 46). Os sintomas mais freqüentes foram déficits motores (53,8%), alterações cognitivas (53,8%), emagrecimento (46,1%), cefaléia (46,1%) e crises convulsivas (46,1%). O diagnóstico foi confirmado pela detecção do P. brasiliensis no SNC. Todos os pacientes apresentavam a forma granulomatosa e quatro (30,8%) tinham a forma meningoencefalítica associada. Todos foram estudados com tomografia computadorizada (TC) de crânio e um caso com ressonância magnética (RM) encefálica. Dez pacientes (76,9%) realizaram sorologia para o HIV, todos com resultados negativos. A anfotericina B foi utilizada em 12 casos (92,3%), em um deles por via intratecal. Em oito casos (61,5%) o sulfametoxazol-trimetropim foi utilizado; em dois (15,4%) a sulfadiazina e pirimetamina, e o fluconazol, cetoconazol e itraconazol, cada um deles em um paciente. Seis pacientes (46,1%) morreram e sete evoluíram satisfatoriamente. O tempo de seguimento variou de 2 a 74 meses (M=30,9). Conclui-se que as manifestações clínicas assim como os exames de imagem na PCM do SNC são inespecíficos.

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“…Some phenotypic characteristics of BAT isolate were evaluated. Micromorphological aspects, virulence for guinea pig, and serological reaction of BAT-isolate exoantigen with rabbit anti GP43 serum were typical traits of P. brasiliensis strains (Lacaz CS et al, 1999). Compared to other P. brasiliensis isolates, BAT isolate had high exoantigen production, which was recognized by sera from patients with PCM in southeastern Brazil (Silva-Vergara et al, 1998;Panunto-Castelo et al, 2003).…”

Section: Discussionmentioning

confidence: 94%

Characterization of a Paracoccidioides spp. strain from southeastern Brazil genotyped as Paracoccidioides restrepiensis (PS3) and review of this phylogenetic species

et al. 2020

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Phylogenetic species of Paracoccidioides brasiliensis complex (S1a and S1b, PS2, PS3, and PS4) and Paracoccidioides lutzii are agents of paracoccidioidomycosis, an endemic fungal disease in Latin America. P. restrepiensis (PS3 genotype) was classified as monophyletic and geographically restricted to Colombia and neighboring territories. BAT (or Pb-327B) was isolated from a patient living in the southeast region of Brazil but with genotype similar to Colombian Paracoccidioides spp. strains. This study aimed to define the phylogenetic species of BAT isolate by using additional genotyping methods, as well as reviewing the epidemiological and clinical studies related to P. restrepiensis isolates. Genomic DNA of BAT isolate and reference strains of P. brasiliensis sensu stricto (S1b), P. americana (PS2), P. restrepiensis (PS3), and P. lutzii were analyzed by conventional polymerase chain reaction (PCR) of partial gp43 exon 2 loci, by PCR-RFLP technique of tub1 gene, and by sequencing of the whole gp43 exon 2 loci. Here, we show that BAT isolate belongs to P. restrepiensis species, which is an unusual identification in southeastern Brazil, where P. brasiliensis sensu stricto is the prevalent genotype. This identification has relevance for geographical distribution and propagation of the genus Paracoccidioides in South America.

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Paracoccidioides brasiliensis: A MYCOLOGIC AND IMMUNOCHEMICAL STUDY OF TWO STRAINS

Cited by 4 publications

References 15 publications

Updates in Paracoccidioides Biology and Genetic Advances in Fungus Manipulation

Updates in Paracoccidioides Biology and Genetic Advances in Fungus Manipulation

Paracoccidioidomicose do sistema nervoso central: análise de 13 casos

Characterization of a Paracoccidioides spp. strain from southeastern Brazil genotyped as Paracoccidioides restrepiensis (PS3) and review of this phylogenetic species

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