Random amplified polymorphic DNA (RAPD) analysis of Lutzomyia longipalpis laboratory populations

Abstract

The phlebotomine sand fly Lutzomyia longipalpis has been incriminated as a vector of American visceral leishmaniasis, caused by Leishmania chagasi. However, some evidence has been accumulated suggesting that it may exist in nature not as a single but as a species complex. Our goal was to compare four laboratory reference populations of L. longipalpis from distinct geographic regions at the molecular level by RAPD-PCR. We screened genomic DNA for polymorphic sites by PCR amplification with decamer single primers of arbitrary nucleotide sequences. One primer distinguished one population (Marajó Island, Pará State, Brazil) from the other three (Lapinha Cave, Minas Gerais State, Brazil; Melgar, Tolima Department, Colombia and Liberia, Guanacaste Province, Costa Rica). The population-specific and the conserved RAPD-PCR amplified fragments were cloned and shown to differ only in number of internal repeats.

Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

“…A total of 83 intraspecific studies has been recorded in the literature (Esseghir et al, 1997;Marcondes et al, 1997;Dias et al, 1998;Aransay et al, 2000Aransay et al, , 2003Di Muccio et al, 2000;Esseghir and Ready, 2000;Mukhopadhyay et al, 2000;Yin et al, 2000;Marquez et al, 2001;Soto et al, 2001;Arrivillaga et al, 2002Arrivillaga et al, , 2003Bauzer et al, 2002a,b;Depaquit et al, , 2004aDepaquit et al, , 2005bDepaquit et al, , 2008bDepaquit et al, , 2013Depaquit et al, , 2014Hodgkinson et al, 2002Hodgkinson et al, , 2003Testa et al, 2002;Bottecchia et al, 2004;Margonari et al, 2004;Pesson et al, 2004;Yahia et al, 2004;Elnaiem et al, 2005;Meneses et al, 2005;Perrotey et al, 2005;Dvorak et al, 2006Dvorak et al, , 2011Mazzoni et al, 2006Mazzoni et al, , 2008de Souza Rocha et al, 2007;Hamarsheh et al, 2007;Moin-Vaziri et al, 2007a,b;Baron et al, 2008;Bounamous et al, 2008Bounamous et al, , 2014Lins et al, 2008…”

“…Their first application to the systematics of Phlebotomine sandflies was done by pioneers in the 1990's (Adamson et al, 1991;Booth et al, 1991;Adamson et al, 1993;Maingon et al, 1993;Zeledon et al, 1993;Booth et al, 1996;Esseghir et al, 1997;Friedrich and Tautz, 1997;Depaquit et al, 1998;Dias et al, 1998). They constitute powerful tools to define populations with evident consequences to characterise vectors and non-vectors, to emphasise cryptic species, to associate males with females in a same species or to propose evolutionary systematics.…”

Genetic differentiation in natural populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae) with different phenotypic spot patterns on tergites in males

“…Other variations in populations of L. longipalpis from Central and South America have been reported, such as behavioral, morphological and biochemical characteristics (Dias et al 1998, Lampo et al 1999, Araki et al 2009, and the existence of reproductive isolation among populations from Central and South America mediated by different sex pheromones has been already suggested (Ward et al 1988).…”

Ecology of the Leishmania Species in the Guianan Ecoregion Complex

“…111 This vector may exist as a species complex with different genetic profiles and biologic properties. [112][113][114][115][116][117] Lutzomyia longipalpis is typically a peri-domestic phlebotomine sand fly in dry regions. American visceral leishmaniasis has not been associated with forested regions.…”