IgM and IgA Antibody Responses in 12 Cases of Human Acquired Toxoplasmosis

Takahashi, Emília Emiko Hieda; Rossi, Claudio

doi:10.1590/s0036-46651997000600004

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…Development of more sensitive and discriminative techniques are needed to clear the immunologic status of individuals infected with T. gondii. At the present time, IgM antibody is not considered as a reliable marker of early infection, since several studies have demonstrated persistence of IgM antibodies for long time 22,32 .…”

Section: Discussionmentioning

confidence: 99%

Seroepidemiology of toxoplasmosis in women of childbearing age from a marginal community of Maracaibo, Venezuela

Díaz-Suárez

Estévez

2009

Rev. Inst. Med. trop. S. Paulo

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SuMMarYThe aim of this study was to determine the prevalence of anti-T. gondii total and IgM antibodies in women of childbearing age. One hundred serum samples of women were studied with age range from 11 to 45 years old. Samples were chosen by random. The determination of total antibodies was carried out through the indirect hemagglutination technique and IgM antibodies by ELISA's technique. The statistical analysis was carried out through the Chi square and the Spearman correlation tests. The theoretical estimated incidence of congenital toxoplasmosis was calculated, according to the annual increment of antibody prevalence among the age groups. The overall prevalence of toxoplasmosis was 33%, while only six individuals (18.2%) were positive to IgM. The highest prevalence was observed in the 11-35 year-old age group. The theoretical estimated incidence was 1.5 for 100 pregnancies in women of 21-25 year-old group; it decreased until 0.1% in the 41-45 year-old age group. The findings show a high prevalence of toxoplasmosis in this community with a high infection risk in women of the studied age group and the high cat population observed, suggesting that the transmission way by contaminated soils may play a main role in the spreading of toxoplasmosis in this community.

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Section: Discussionmentioning

confidence: 99%

Seroepidemiology of toxoplasmosis in women of childbearing age from a marginal community of Maracaibo, Venezuela

Díaz-Suárez

Estévez

2009

Rev. Inst. Med. trop. S. Paulo

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“…At this cut-off value, signi®cant levels of IgA were detected for 1 year after the beginning of clinical manifestations. IgA levels < 40 AU=ml with the ETI-TOXOK-A kit do not exclude a recent infection, as in some patients such levels can be reached very soon after infection with T. gondii [34]. Decoster et al [35] reported no signi®cant differences in the sensitivities and speci®cities of the Platelia IgA and the IMx Toxo IgA kits for the diagnosis of acquired toxoplasmosis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of serological markers for the immunodiagnosis of acute acquired toxoplasmosis

Suzuki¹,

Rocha

Rossi³

2001

Journal of Medical Microbiology

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The detection of speci®c IgM antibodies has been the most frequently used serological marker for diagnosing recent toxoplasmosis. However, the persistence of speci®c IgM antibodies in some patients and the use of tests with a low speci®city have complicated the interpretation of serological results when toxoplasmosis is suspected. The purpose of the present study was to determine the value of newer serological techniques in the diagnosis of acute acquired toxoplasmosis. Sixty-four sera, 31 from patients with Toxoplasma gondii infection and 33 from patients with latent infection, were tested. Anti-T. gondii IgA was measured by two antibody capture ELISA tests (Platelia 1 Toxo IgA and ETI-TOXOK A) and an automated direct ELISA (IMx 1 Toxo IgA); all three assays detected antibody levels compatible with a recent infection in sera from all 31 patients with acute toxoplasmosis. However, signi®cant levels of IgA were also detected with high frequency by all three assays in sera from patients with latent infection. IgE antibodies detected by IgE immunosorbent agglutination assay (ISAGA) were present in 26 (84%) of 31 patients with acute toxoplasmosis and in sera from two subjects with latent infection taken >1 year after the beginning of the clinical symptoms of infection. Thirty (97%) of 31 patients with a recent T. gondii infection and 15 (45%) of 33 subjects with latent infection had an AC/HS pattern compatible with acute toxoplasmosis. The avidity of T. gondii IgG was evaluated by two methods. One method was based on the titration of each serum sample and calculation of the titres, in the absence and presence of urea, in relation to a de®ned cut-off value. In the other method, a single serum dilution was used and the absorbances of the reactions in the presence and absence of urea were compared. The titration method was more sensitive for diagnosing recent primary infection; all 31 sera from patients with acute toxoplasmosis had avidity indices compatible with acute toxoplasmosis by the titration method, whereas with the single dilution method, sera from four patients had equivocal results. In the 33 individuals with latent infection, similar results were obtained with the two avidity methods; only one serum sample had a non-compatible avidity value with the titration method. The results obtained in the present study show that the current serological markers used for diagnosing acute acquired toxoplasmosis have signi®cant limitations. The data suggest that determination of the avidity of T. gondii-speci®c IgG by the titration method in patients with detectable IgM antibodies de®nes most accurately the stage of infection by T. gondii.

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“…Anti-T. gondii IgA was measured by enzymelinked immunosorbent assay (ELISA) using Platelia TM Toxo IgA kits (Bio-Rad, France). IIF was done as previously described 9 , and titers ≥ 32 were considered positive. ELFA and ELISA were performed according to the manufacturers' instructions.…”

Section: Case Reportmentioning

confidence: 99%

Serological monitoring of a Toxoplasma infection after hematopoietic stem cell transplantation

Rossi

Nascimento

Barros‐Mazon

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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IgM and IgA Antibody Responses in 12 Cases of Human Acquired Toxoplasmosis

Cited by 4 publications

References 22 publications

Seroepidemiology of toxoplasmosis in women of childbearing age from a marginal community of Maracaibo, Venezuela

Seroepidemiology of toxoplasmosis in women of childbearing age from a marginal community of Maracaibo, Venezuela

Evaluation of serological markers for the immunodiagnosis of acute acquired toxoplasmosis

Serological monitoring of a Toxoplasma infection after hematopoietic stem cell transplantation

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