Oviposition by Schistosoma mansoni during in vitro cultivation

Barth, L R; Fernandes, Ana Paula; Rodrigues, Vanderlei

doi:10.1590/s0036-46651996000600006

Cited by 18 publications

(8 citation statements)

References 8 publications

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“…In mice fed a high-fat diet, an initial low elimination was observed between 6-8 weeks, followed by significant increases at weeks 9 and 10, with week-to-week fluctuations from week 11-16, when excretion was increased. The main differences were the absence of stability and a progressive decrease, as previously reported during the chronic phase of schistosomiasis (Cheever et al 1994, Rocha et al 1995, Barth et al 1996. This study complements the existing literature (Neves et al 2007a, b) indicating that certain dietary patterns may be important for the outcome of chronic schistosomiasis.…”

Section: Discussionsupporting

confidence: 86%

High fat diet has a prominent effect upon the course of chronic schistosomiasis mansoni in mice

Alencar

Neves

Águila³

et al. 2009

Mem. Inst. Oswaldo Cruz

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This study investigated whether a long-term high-fat diet has an effect on the outcome of chronic murine schistosomiasis mansoni compared to a standard diet. Swiss Webster female mice (3 weeks old) were fed each diet for up to six months and were then infected with 50 Schistosoma mansoni cercariae. Their nutritional status was assessed by monitoring total serum cholesterol and body mass. Infected mice were examined 6-17 weeks post infection to estimate the number of eggs in faeces. Mice were euthanised the next day. Total serum cholesterol was lower in infected mice in comparison to uninfected controls (p = 0.0055). In contrast, body mass (p = 0.003), liver volume (p = 0.0405), spleen volume (p = 0.0124), lung volume (p = 0.0033) and faecal (p = 0.0064) and tissue egg density (p = 0.0002) were significantly higher for infected mice fed a high-fat diet. From these findings, it is suggested that a high-fat diet has a prominent effect on the course of chronic schistosomiasis mansoni in mice.

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Section: Discussionsupporting

confidence: 86%

High fat diet has a prominent effect upon the course of chronic schistosomiasis mansoni in mice

Alencar

Neves

Águila³

et al. 2009

Mem. Inst. Oswaldo Cruz

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“…The separation of couples of S. mansoni exposed to drugs is important. According to Barth et al 47 drug exposure can cause changes in reproductive ability, changes in the female's vitelline cells and, consequently, a decrease in egg production.…”

Section: In Vitro Biological Assaysmentioning

confidence: 99%

Nanostructured Lipid Carriers as a Strategy to Improve the <I>In Vitro</I> Schistosomiasis Activity of Praziquantel

Kolenyak-Santos¹,

Garnero²,

Oliveira³

et al. 2015

j nanosci nanotechnol

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Praziquantel (PZQ) is a pyrazinoisoquinoline anthelmintic that was discovered in 1972 by Bayer Germany. Currently, due to its efficacy, PZQ is the drug of choice against all species of Schistosoma. Although widely used, PZQ exhibits low and erratic bioavailability because of its poor water solubility. Nanostructured lipid carriers (NLC), second-generation solid lipid nanoparticles, were developed in the 1990s to improve the bioavailability of poorly water soluble drugs. The aim of this study was to investigate nanostructured lipid carriers as a strategy to improve the efficacy of PZQ in S. mansoni treatment. We prepared NLC2 and NLC4 by adding seventy percent glycerol monostearate (GMS) as the solid lipid, 30% oleic acid (OA) as the liquid lipid and two surfactant systems containing either soybean phosphatidylcholine/poloxamer (PC/P-407) or phosphatidylcholine/Tween 60 (PC/T60), respectively. The carriers were characterized by nuclear magnetic resonance, differential scanning calorimetry, thermogravimetric analysis and Fourier transform-infrared spectroscopy. The safety profile was evaluated using red cell hemolysis and in vitro cytotoxicity assays. The results showed that the encapsulation of PZQ in NLC2 or NLC4 improved the safety profile of the drug. Treatment efficacy was evaluated on the S. mansoni BH strain. PZQ-NLC2 and PZQ-NLC4 demonstrated an improved efficacy in comparison with free PZQ. The results showed that the intestinal transport of free PZQ and PZQ-NLC2 was similar. However, we observed that the concentration of PZQ absorbed was smaller when PZQ was loaded in NLC4. The difference between the amounts of absorbed PZQ could indicate that the presence of T60 in the nanoparticles (NLC4) increased the rigid lipid matrix, prolonging release of the drug. Both systems showed considerable in vitro activity against S. mansoni, suggesting that these systems may be a promising platform for the administration of PZQ for treating schistosomiasis.

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“…In this work, the number of eggs per gram of feces in the schistosomotic animals with or without splenectomy was higher on the 45 th compared with the 97 th day of infection. According to the literature, this period corresponds with the onset of chronic infection in mice (29) (30) .…”

Section: Discussionmentioning

confidence: 95%

“…The development of the disease caused by S. mansoni has been described to begin with low egg laying, which increases gradually between 45 and 72 days and later decreases at three weeks after the incubation period (29) (30) . In this work, the number of eggs per gram of feces in the schistosomotic animals with or without splenectomy was higher on the 45 th compared with the 97 th day of infection.…”

Section: Discussionmentioning

confidence: 99%

Post-splenectomy infections in chronic schistosomiasis as a consequence of bacterial translocation

Lima

Negro-Dellacqua

Santos

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:Bacterial translocation is the invasion of indigenous intestinal bacteria through the gut mucosa to normally sterile tissues and internal organs. Schistosomiasis may cause alterations in the immune system and damage to the intestines, portal system and mesenteric lymph nodes. This study investigated bacterial translocation and alterations in the intestinal microbiota and mucosa in schistosomiasis and splenectomized mice. Methods: Forty female 35-day-old Swiss Webster mice were divided into the following four groups with 10 animals each: schistosomotic (ESF), splenectomized schistosomotic (ESEF), splenectomized (EF) and control (CF). Infection was achieved by introduction of 50 Schistosoma mansoni (SLM) cercariae through the skin. At 125 days after birth, half of the parasitized and unparasitized mice were subjected to splenectomy. Body weights were recorded for one week after splenectomy; then, the mice were euthanized to study bacterial translocation, microbiota composition and intestinal morphometry. Results: We observed signifi cant reductions in the weight increases in the EF, ESF and ESEF groups. There were increases of at least 1,000 CFU of intestinal microbiota bacteria in these groups compared with the CF. The EF, ESF and ESEF mice showed decreases in the heights and areas of villi and the total villus areas (perimeter). We observed frequent co-infections with various bacterial genera. Conclusions: The ESEF mice showed a higher degree of sepsis. This fi nding may be associated with a reduction in the immune response associated with the absence of the spleen and a reduction in nutritional absorption strengthened by both of these factors (Schistosoma infection and splenectomy).

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Oviposition by Schistosoma mansoni during in vitro cultivation

Cited by 18 publications

References 8 publications

High fat diet has a prominent effect upon the course of chronic schistosomiasis mansoni in mice

High fat diet has a prominent effect upon the course of chronic schistosomiasis mansoni in mice

Nanostructured Lipid Carriers as a Strategy to Improve the <I>In Vitro</I> Schistosomiasis Activity of Praziquantel

Post-splenectomy infections in chronic schistosomiasis as a consequence of bacterial translocation

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