1995
DOI: 10.1590/s0036-46651995000100006
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Abstract: Parasitological diagnosis based on the faecal examination is frequently difficult in cases of chronic, low-level S. stercoralis infection. Even when a newly developed sensitive method (an agar plate culture) is applied, it is essential to examine faecal samples repeatedly to achieve a correct diagnosis. Additionally, it is important to note that a negative result does not necessarily indicate the unequivocal absence of the infection. On the other hand, several serological tests which have recently been develop… Show more

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Cited by 44 publications
(38 citation statements)
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“…Complementary tests for the diagnosis and the monitoring of the immune response in this parasitosis have been developed. However, the major limitation for the standardization of immunological methods is the difficulty in obtaining large amount of S. stercoralis larvae (Sato et al 1995, Costa-Cruz et al 1997.The aim of this study was to diagnose human strongyloidiasis by enzyme-linked immunosorbent assay (ELISA) using alkaline extract of S. venezuelensis filariform larvae. The study received approval from the Ethical Committee of the Federal University of Uberlândia.…”
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confidence: 99%
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“…Complementary tests for the diagnosis and the monitoring of the immune response in this parasitosis have been developed. However, the major limitation for the standardization of immunological methods is the difficulty in obtaining large amount of S. stercoralis larvae (Sato et al 1995, Costa-Cruz et al 1997.The aim of this study was to diagnose human strongyloidiasis by enzyme-linked immunosorbent assay (ELISA) using alkaline extract of S. venezuelensis filariform larvae. The study received approval from the Ethical Committee of the Federal University of Uberlândia.…”
mentioning
confidence: 99%
“…Complementary tests for the diagnosis and the monitoring of the immune response in this parasitosis have been developed. However, the major limitation for the standardization of immunological methods is the difficulty in obtaining large amount of S. stercoralis larvae (Sato et al 1995, Costa-Cruz et al 1997.…”
mentioning
confidence: 99%
“…IgE anti-S. stercoralis antibodies have been detected by radioallergosorbent test (RAST), enzyme-linked immunosorbent assay (ELISA), and immunoblotting (IB) (McRury et al 1986, Conway et al 1993, Sato et al 1995, Atkins et al 1997. However, one of the most important limitations for immunodiagnosis in strongyloidiasis is the difficulty for obtaining S. stercoralis filariform larvae.…”
mentioning
confidence: 99%
“…Immunodiagnosis of S. stercoralis infection has been attempted with several serological tests. Enzyme linked immunosorbent assay (ELISA) permits the detection of serum specific IgG 1 , and can be used as an epidemiological tool to determine the prevalence of strongyloidiasis in a given region or population 18,19,20 . In Chile, ELISA test to detect S. stercoralis serum antibodies against an alkaline protein extract of the filariform larvae of S. venezuelensis was standardized 16 .…”
Section: Introductionmentioning
confidence: 99%