Interaction between neutrophils and Schistosoma mansoni larvae in vivo: a transmission electron-microscopic study

Melo, Alan Lane de; Machado, Conceição Ribeiro da Silva; Pereira, Leandro de Mattos

doi:10.1590/s0036-46651993000100005

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…Since it is known that neutrophils adhere to the larval schistosomes’ surface and participate in the removal of glycocalyx by phagocytosis in vivo [ 26 ] and our studies indicates that Sm KI-1 seems to impair elastase secretion by neutrophils, we depleted neutrophils in vivo and infected mice with Sm KI-1-suppressed schistosomula or control parasites. When worms recovered from animals infected with Sm KI-1-suppressed schistosomula were compared to parasites treated with control siRNA in neutrophils depleted mice, we still observed a worm burden reduction of 16% suggesting that Sm KI-1 plays an important role in parasite development independent of its ability to inhibit host neutrophils in vivo ( S3 Fig ).…”

Section: Resultsmentioning

confidence: 99%

Schistosoma mansoni SmKI-1 serine protease inhibitor binds to elastase and impairs neutrophil function and inflammation

et al. 2018

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Protease inhibitors have important function during homeostasis, inflammation and tissue injury. In this study, we described the role of Schistosoma mansoni SmKI-1 serine protease inhibitor in parasite development and as a molecule capable of regulating different models of inflammatory diseases. First, we determine that recombinant (r) SmKI-1 and its Kunitz domain but not the C-terminal region possess inhibitory activity against trypsin and neutrophil elastase (NE). To better understand the molecular basis of NE inhibition by SmKI-1, molecular docking studies were also conducted. Docking results suggest a complete blockage of NE active site by SmKI-1 Kunitz domain. Additionally, rSmKI-1 markedly inhibited the capacity of NE to kill schistosomes. In order to further investigate the role of SmKI-1 in the parasite, we designed specific siRNA to knockdown SmKI-1 in S. mansoni. SmKI-1 gene suppression in larval stage of S. mansoni robustly impact in parasite development in vitro and in vivo. To determine the ability of SmKI-1 to interfere with neutrophil migration and function, we tested SmKI-1 anti-inflammatory potential in different murine models of inflammatory diseases. Treatment with SmKI-1 rescued acetaminophen (APAP)-mediated liver damage, with a significant reduction in both neutrophil recruitment and elastase activity. In the model of gout arthritis, this protein reduced neutrophil accumulation, IL-1β secretion, hypernociception, and overall pathological score. Finally, we demonstrated the ability of SmKI-1 to inhibit early events that trigger neutrophil recruitment in pleural cavities of mice in response to carrageenan. In conclusion, SmKI-1 is a key protein in S. mansoni survival and it has the ability to inhibit neutrophil function as a promising therapeutic molecule against inflammatory diseases.

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Section: Resultsmentioning

confidence: 99%

Schistosoma mansoni SmKI-1 serine protease inhibitor binds to elastase and impairs neutrophil function and inflammation

et al. 2018

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“…In previous in vivo experimental S. mansoni study 15,16 , it was found that intraperitoneal injections of cercariae into naive mice caused a cell interaction (including cluster reactions) to the infective larvae. However, there have been no investigations on cluster of cell reactivity 12,15 following total body g-irradiation.…”

Section: Introductionmentioning

confidence: 97%

“…Some studies have highlighted the macrophages or peritoneal cells as active role during S. mansoni infection by classical routes 3,11 or the neutrophils as an example of the first line defence in a peritoneal cavity of mice model 15,16 . However, little attention has been done to wholebody irradiation protocols in, which are might expect disable innate peritoneal resistance in mice against S. mansoni compared with other radiation protocols 13 .…”

Section: Introductionmentioning

confidence: 99%

Schistosoma mansoni: inflammatory foci around larvae in the peritoneal cavity of naive mice is radiosensitive

Melo

Chamone

2001

Rev. Inst. Med. trop. S. Paulo

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SUMMARYInnate attack to Schistosoma mansoni cercariae was evaluated in irradiated mice. It was observed that 70% of the larvae from mice sacrificed one day after whole body irradiation with 400 or 800 rads were surrounded by cluster reactivities, without difference from controls. Differences were apparent on day 5 after irradiation with sub lethal (400 rads) or lethal doses (800 rads) suggesting that innate defence to infection take at least 5 days to be affected by low dose whole-body radiation.

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“…As an advantage, peritoneal infection allows a simple and rapid method for mass screening of prophylactic agents to combat schistosomiasis (Pereira et al , 1974). This method of study has also helped to elucidate aspects of the early stages and immunology of the host–parasite relationship (Melo et al , 1978, 1980, 1993a, b, 1994; Melo & Pereira, 1980, 1985; Chao et al , 1986). Indeed, it has been demonstrated that parasites recovered from the peritoneal cavity of two albino mouse strains (AKR/J and Swiss) are significantly different in relation to the total body length of the worms, the intraperitoneal worm burden and the maturation degree of the schistosomes (Bicalho et al , 1993).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic plasticity of maleSchistosoma mansonifrom the peritoneal cavity and hepatic portal system of laboratory mice and hamsters

et al. 2014

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Morphometric analysis of Schistosoma mansoni male worms obtained from AKR/J and Swiss mice was carried out. Rodents infected by the intraperitoneal route with 80 cercariae of the schistosome (LE strain) were killed by cervical dislocation at 45 and 60 days post-infection and both peritoneal lavage and perfusion of the portal system were performed for the recovery of adult worms. Characteristics including total body length, the distance between oral and ventral suckers, extension of testicular mass and the number of testes were considered in the morphological analysis. Changes that occurred in S. mansoni recovered from the peritoneal cavity or from the portal system of AKR/J and Swiss mice included total body length and reproductive characteristics. Significant morphometric alterations were also observed when worms recovered from the portal system of both strains of mice were compared with the schistosomes obtained from hamsters (Mesocricetus auratus), the vertebrate host in which the LE strain had been adapted and maintained by successive passages for more than four decades. The present results reinforce the idea that S. mansoni has high plastic potential and adaptive capacity.

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Interaction between neutrophils and Schistosoma mansoni larvae in vivo: a transmission electron-microscopic study

Cited by 5 publications

References 20 publications

Schistosoma mansoni SmKI-1 serine protease inhibitor binds to elastase and impairs neutrophil function and inflammation

Schistosoma mansoni SmKI-1 serine protease inhibitor binds to elastase and impairs neutrophil function and inflammation

Schistosoma mansoni: inflammatory foci around larvae in the peritoneal cavity of naive mice is radiosensitive

Phenotypic plasticity of maleSchistosoma mansonifrom the peritoneal cavity and hepatic portal system of laboratory mice and hamsters

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