1993
DOI: 10.1590/s0036-46651993000100003
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Abstract: Cercariae of Schistosoma mansoni inoculated into the peritoneal cavity of naive mice induced host cell adhesion to their surface, but after 90 minutes the number of adherent cells sharply decreased. The cell detachment is progressive and simultaneous to the cercaria-schistosomule transformation. The histological study showed mainly neutrophils in close contact with the larvae. Mononuclear cells and some eosinophils were occasionally seen surrounding the adherent neutrophils. The scanning electron microscopy sh… Show more

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Cited by 8 publications
(2 citation statements)
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“…After washing, the sample was post-fixed in 1% osmium tetroxide in 0.1 M sodium cacodylate buffer (pH 7.2) + 1.5% potassium ferrocyanide for 90 minutes, washed with 0.1 M with sodium cacodylate buffer (pH 7.2), dehydrated in a graduated ethanol series (50% EtOH, 70% EtOH, 95% EtOH, and 100% EtOH), and incorporated in Eponate–Araldite resin. Ultrathin sections were obtained using uranyl acetate and lead citrate and then verified by Zeiss-EM-10A [97]. The micrograph was obtained by one CCD Mega View camera.…”
Section: Methodsmentioning
confidence: 99%
“…After washing, the sample was post-fixed in 1% osmium tetroxide in 0.1 M sodium cacodylate buffer (pH 7.2) + 1.5% potassium ferrocyanide for 90 minutes, washed with 0.1 M with sodium cacodylate buffer (pH 7.2), dehydrated in a graduated ethanol series (50% EtOH, 70% EtOH, 95% EtOH, and 100% EtOH), and incorporated in Eponate–Araldite resin. Ultrathin sections were obtained using uranyl acetate and lead citrate and then verified by Zeiss-EM-10A [97]. The micrograph was obtained by one CCD Mega View camera.…”
Section: Methodsmentioning
confidence: 99%
“…As an advantage, peritoneal infection allows a simple and rapid method for mass screening of prophylactic agents to combat schistosomiasis (Pereira et al , 1974). This method of study has also helped to elucidate aspects of the early stages and immunology of the host–parasite relationship (Melo et al , 1978, 1980, 1993a, b, 1994; Melo & Pereira, 1980, 1985; Chao et al , 1986). Indeed, it has been demonstrated that parasites recovered from the peritoneal cavity of two albino mouse strains (AKR/J and Swiss) are significantly different in relation to the total body length of the worms, the intraperitoneal worm burden and the maturation degree of the schistosomes (Bicalho et al , 1993).…”
Section: Introductionmentioning
confidence: 99%