Serological, electrophoretic and biological properties of Fasciola hepatica antigens

Cervi, Laura; Rubinstein, H.R.; Masih, Diana T.

doi:10.1590/s0036-46651992000600005

Cited by 14 publications

(3 citation statements)

References 13 publications

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“…(prominent bands in WB extract with the range of 12–95 kDa), Gigantocotyle explanatum, Gastrothylax crumenifer and Paramphistomum sp. (prominent bands in WB extract within the range of 11·5–200 kDa) .…”

Section: Discussionmentioning

confidence: 99%

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

Anuracpreeda

Chawengkirttikul

Sobhon

2016

Parasite Immunology

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An antigenic component of adult Paramphistomum gracile was characterized by means of indirect enzyme-linked immunosorbent assay (indirect ELISA), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from cattle naturally infected with P. gracile, Eurytrema pancreaticum, Fasciola gigantica, Moniezia benedeni, strongylids, Trichuris sp. and Strongyloides sp. The whole body (WB) extracts of P. gracile were fractionated by gel filtration chromatography in a Sephadex G-200 column. It was found that the WB extract fractions, F1-F3 were highly antigenic, F5 was moderately antigenic and F4 was poorly antigenic. For SDS-PAGE and immunoblotting, the antigenic molecules of WB extract and all five fractions were mostly at molecular weights (MW) ranging from 12 to 150 kDa. One antigenic protein of 16 kDa detected in WB extract and F1-F3 was found to give a consistent reaction with sera from infected cattle. The antigenicity of the purified 16 kDa protein was confirmed by immunoblotting and indirect ELISA using a pool of sera and individual serum samples from infected cattle (at 1 : 78 125 dilution) and hyperimmunized rabbit (at 1 : 390 625 dilution). This finding suggests that the 16 kDa protein may be a potential antigen for the immunodiagnosis of cattle paramphistomosis caused by P. gracile.

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Section: Discussionmentioning

confidence: 99%

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

Anuracpreeda

Chawengkirttikul

Sobhon

2016

Parasite Immunology

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“…The number and molecular weight range of protein profile of whole worm extract of F. gigantica in our study was similar to the closely related species. For example, the study carried out by Cervi et al, 1992 in case of F. hepatica revealed the somatic protein band pattern within the range of 14 – 94 kDa.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and Immunodiagnostic potential of various antigenic proteins of Fasciola gigantica species isolated from sheep of North West Himalayan Region

et al. 2019

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SummaryThe control of the digenetic trematode Fasciola gigantica has been the major challenge in both cattle and small ruminants as there is a paucity of an effective and commercial vaccine. Thus, the accurate identification and prepatent diagnosis of F. gigantica is an essential prerequisite for its successful prevention and control. In the present study, the morphologically identified specimens isolated from the liver and bile ducts of sheep (Ovis aries) were validated through molecular data. The sequence analysis of ITS2 of our isolates showed high degree of similarity with F. gigantica and F. hepatica using BLAST function of NCBI. The phylogenetic analysis of our isolates showed a close relationship with previously described F. gigantica and F. hepatica isolates from different countries. The antigenic profile of somatic and E/S antigens of F. gigantica were revealed by SDS–PAGE and immunoblotting using sera from sheep naturally infected with F. gigantica. By SDS-PAGE, 20 distinct bands were revealed from crude somatic fraction. Immunoblotting analysis of these proteins with positive sera exhibited 8 sero-reactive bands ranging from 14 to 97 kDa. Among these 38 and 44 kDa bands were quite specific with high diagnostic specificity and sensitivity. The E/S fraction comprised 7 distinct bands, as revealed by SDS-PAGE analysis. Immunoblotting analysis of these proteins with positive sera exhibited 6 antigenic bands ranging from 23 – 54 kDa. Among these 27 and 33 kDa were found to be quite specific with high diagnostic specificity and sensitivity. The present study concludes that the protein bands of 38 and 44 kDa in somatic fraction and 27 and 33 kDa in E/S fraction can be used for the immunodiagnostic purpose for this economically important parasite, which may also entice further studies regarding their vaccine potential.

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“…Antigens TH of F. hepatica was prepared according to a procedure described by Cervi et al (1992). Briefly, adult F. hepatica worms were obtained from infected bovine livers at the local abattoir and homogenized by mechanical disruption in a tissue grinder containing phenylmethyl-sulphonyl fluoride.…”

Section: Methodsmentioning

confidence: 99%

Immunization with crude antigens plus aluminium hydroxide protects cattle fromFasciola hepaticainfection

et al. 2011

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The ability of total homogenate (TH) of Fasciola hepatica conjugated with aluminium hydroxide (alum) or Freund's complete adjuvant (FCA) to protect cattle against experimental fasciolosis was evaluated. Compared with the infected group, the immunized animals with alum-TH and FCA-TH presented a significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher percentage of short-sized worms, a marked reduction in the released eggs in faeces (89% and 57%, respectively), as well as an increased production of specific antibodies before infection. The alum-TH immunized group also showed a significant increase in the antigen-specific proliferation of peripheral blood mononuclear cells (PBMC) as early as 4 weeks before infection. Although both immunized groups (alum-TH and FCA-TH) were able to develop an efficient protective immune response to metacercarial challenge, an earlier PBMC response, lower hepatic damage and less effect on weight gain were found in alum-immunized animals. Therefore, alum is a good candidate for future immunization against bovine fasciolosis.

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Serological, electrophoretic and biological properties of Fasciola hepatica antigens

Cited by 14 publications

References 13 publications

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

Molecular characterization and Immunodiagnostic potential of various antigenic proteins of Fasciola gigantica species isolated from sheep of North West Himalayan Region

Immunization with crude antigens plus aluminium hydroxide protects cattle fromFasciola hepaticainfection

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