Isolation of urease-positive Vibrio parahaemolyticus from diarrheal patients in Northeast Brazi

Magalhães, Marcelo; Magalhäes, Vera; Antas, Maria G.; Tateno, Seiki

doi:10.1590/s0036-46651991000400004

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…In the present study it was observed that one strain showed beta-hemolysis on Wagatsuma agar (Kanagawa positive) but it was negative for tdh gene. This phenomenon has been reported in Brazil 1,4,16,24,32 and might be due to deletion or mutation in the tdh gene 33 .…”

Section: Discussionmentioning

confidence: 55%

Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil

Rojas

Matté

Dropa

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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SUMMARYVibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) encoded by tdh and trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters and mussels were analyzed by PCR using specific primers for the 16S rRNA and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and PFGE. Nineteen isolates were confirmed by PCR as V. parahaemolyticus. The tlh gene was present in 100% of isolates, the tdh gene was identified in two (10.5%) isolates, whereas the gene trh was not detected. Each isolate was resistant to at least one of the nine antimicrobials tested. Additionally, all isolates possessed the bla TEM-116 gene. The presence of this gene in V. parahaemolyticus indicates the possibility of spreading this gene in the environment. Atypical strains of V. parahaemolyticus were also detected in this study.

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Section: Discussionmentioning

confidence: 55%

Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil

Rojas

Matté

Dropa

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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“…parahaemolyticus, isolated in the same period and regions but not related epidemiologically, were studied as those of the isolated cases (out group). The same serogroups were present, pointing out that the serotype O3:K6 had not been reported in the northeast region and that type O3:KUT was cited on one occasion as the causative agent of gastroenteritis by Magalhães et al. (1991a,b, 1992), although no reference was made in these latter investigations to the predominance of one particular serogroup/serotype.…”

Section: Discussionmentioning

confidence: 93%

“…In relation to Brazil, there are few references to V. parahaemolyticus in intestinal infections (Hofer 1983; Magalhães et al. 1991a,b; 1992) and in the colonization of cutaneous wounds (Rodrigues et al.…”

Section: Introductionmentioning

confidence: 99%

Vibrio parahaemolyticusserovar O3:K6 gastroenteritis in northeast Brazil

Leal¹,

Silva²,

Cavalcanti

et al. 2008

Journal of Applied Microbiology

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Aims: To examine the virulence factors and the genetic relationship isolates of the serogroup O3 of Vibrio parahaemolyticus in outbreaks of diarrhoea in the northeast region of Brazil. Methods and Results: Eighteen samples of the O3:K6 and O3:KUT serotypes of V. parahaemolyticus were analysed by multiplex polymerase chain reaction (m‐PCR) for detection of the tl, tdh and trh genes, by random‐amplified polymorphic DNA (RAPD) using two primers, and by amplification of the rDNA 16S–23S region. The gene tl was amplified in all the samples, tdh in 16 while trh in none; amplification of rDNA 16S–23S generated only one profile; each RAPD primer produced two amplification patterns allowing grouping two tdh– Kanagawa‐negative isolates. Conclusions: V. parahaemolyticus with characteristics of the pandemic clone appears to be widely disseminated in the studied region. Because of the genetic uniformity of the isolates, elucidation of outbreaks or tracking the source of contamination by the present molecular techniques seems useless. Significance and Impact of the Study: Detection of V. parahaemolyticus with virulence potential of pandemic clone from two outbreaks and from several isolated gastroenteritis cases points out the need for inclusion of this micro‐organism in the Brazilian routine monitoring of the diarrhoeas for elucidation of their aetiology.

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“…In relation to Brazil, there are few references to V. parahaemolyticus in cases of intestinal infections of human isolates (Hofer, 1983; Magalhaes et al, 1991a,b, 1992) in the colonization of cutaneous wounds (Rodrigues et al, 2001). In 1991 in Recife, a tropical city situated in Northeast Brazil, V. parahaemolyticus cultures were isolated from 21 cases of gastroenteritis associated with the consumption of different types of seafood.…”

Section: Pandemic O3:k6 Clone On the American Continentmentioning

confidence: 99%

“…In 1991 in Recife, a tropical city situated in Northeast Brazil, V. parahaemolyticus cultures were isolated from 21 cases of gastroenteritis associated with the consumption of different types of seafood. The serotypes detected belonged to diverse serovars, including O1:K56, O3:K5, O3:K58, O3:KUT, O4:K4,O4:K10, O4:K12, O4:K53, O4:KUT, O5:KUT, and O10:KUT (Magalhaes et al, 1991a). In 1992, V. parahaemolyticus strains were identified in 18 human isolates and found to carry either the tdh , trh , or both genes (Magalhaes et al, 1992).…”

Section: Pandemic O3:k6 Clone On the American Continentmentioning

confidence: 99%

Pandemic Vibrio parahaemolyticus O3:K6 on the American continent

Velázquez-Román

León-Sicairos

Hernandez-Diaz³

et al. 2014

Front. Cell. Infect. Microbiol.

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Vibrio parahaemolyticus is one of the most important seafood-borne bacterial in recent years and is the leading causal agent of human acute gastroenteritis, primarily following the consumption of raw, undercooked or mishandled marine products. Until 1996, infections caused by V. parahaemolyticus were generally associated with diverse serovars. However, in February 1996, a unique serovar (O3:K6) of V. parahaemolyticus with specific genetic markers (tdh, toxRS/New and/or orf8) appeared abruptly in Kolkata, India. In subsequent years, O3:K6 isolates similar to those isolated in Kolkata have been reported from food borne outbreaks in Southeast Asia, as well as in the Atlantic and Gulf coasts of the United States (U.S). More recently, there have been reports in Europe, Africa and Central and South America. Specifically, in the American continent, some countries have reported cases of gastroenteritis due to the pandemic O3:K6 strain and its serovariants; the pandemic strain was first detected in Peru (1996, >100 cases), subsequently spreading to Chile in 1998 (>16,804 human cases), to the U.S. in 1998 (>700 cases), to Brazil in 2001 (>18 cases) and to Mexico in 2004 (>1200 cases). The arrival of the pandemic clone on the American continent may have resulted in a significant shift on the epidemic dynamics of V. parahaemolyticus. However, although O3:K6 is the predominant serovar of the recognized clinical strains in some countries in the Americas, a decrease in clinical cases caused by O3:K6 and an increase in cases associated with a new serotype (O3:K59, Chile) have been recently reported. The emergence and worldwide dissemination of O3:K6 and other pandemic strains since 1996 have come to represent a threat to public health and should concern health authorities. This review focuses on the presence, distribution and virulence factors of the V. parahaemolyticus O3:K6 pandemic clone and its serovariants in clinical and environmental strains on the American continent.

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Isolation of urease-positive Vibrio parahaemolyticus from diarrheal patients in Northeast Brazi

Abstract: Of 21 human fecal strains of Vibrio parahaemolyticus, isolated on the Northeast Coast of Brazil, eight (38%) were urease positive. Most of these strains, in contrast to the urease-negative ones, did not produce the hemolysin responsible for the Kanagawa phenomenon.

Cited by 8 publications

References 8 publications

Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil

Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil

Vibrio parahaemolyticusserovar O3:K6 gastroenteritis in northeast Brazil

Pandemic Vibrio parahaemolyticus O3:K6 on the American continent

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