Detección de portadores asintomáticos de quistes hidatídicos: aumento de la especificidad del ensayo inmunoenzimático

Coltorti, E. A.; Fernández, E; Marguet, Emilio Rogelio; Scozzina, J. D.; Guarnera, Eduardo

doi:10.1590/s0036-46651990000400007

Cited by 14 publications

(5 citation statements)

References 15 publications

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“…De acuerdo con los resultados de las pruebas serológicas (HAI y ELISA), los 42 individuos con ambas pruebas positivas representan una seroprevalencia de 754,6 por 100 000 habitantes. En 16 de los 26 casos (61,5%) con HAI y ELISA positivas que se sometieron a la realización de radiografías de tórax y ecografías abdominales, los resultados de estas pruebas de visualización fueron compatibles con el diagnóstico de hidatidosis hepática (10 casos) o pulmonar (6 casos), cifra que es superior al 50,9% comunicado por otros autores (16).…”

Section: Discussionunclassified

“…A pesar de que en nuestro estudio se utilizaron las pruebas de ELISA y HAI, con una especificidad de 95,5% (9) y 96,6% (8), respectivamente, las pruebas de visualización resultaron negativas en 38,5% de los casos con ambas pruebas serológicas positivas. Esta discrepancia entre los resultados de las pruebas serológicas y de visualización podría deberse a la presencia de anticuerpos inespecíficos que aumentarían la incidencia de seropositividad, a la existencia de quistes muy pequeños no detectables por radiografía o ecografía, a localizaciones extraabdominales y extratorácicas o, excepcionalmente, a la curación espontánea del quiste (16).…”

Section: Discussionunclassified

“…Se efectuaron pruebas de hemaglutinación indirecta y ELISA a 5 566 personas aparentemente sanas. Los 42 (0,8%) casos con resultados positivos en ambas pruebas (seroprevalencia de 754,6 por 100 000) fueron citados para ser sometidos a una ecografía hepática y una radiografía de tórax y, de los 26 que acudieron, 16 presentaron imágenes compatibles con quiste hidatídico. Estos 16 casos fueron enviados al hospital para ser intervenidos y en los 9 que acudieron se confirmó el diagnóstico quirúrgicamente, lo cual representa una prevalencia de 161,7 por 100 000.…”

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Equinococosis/hidatidosis en la VII Región de Chile: diagnóstico e intervención educativa

Apt¹,

Vasco²,

Galdamez³

et al. 2000

Rev Panam Salud Publica

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This study was designed to embrace three areas: a) serologic and radiologic diagnosis and surgical treatment of hydatidosis in an asymptomatic human population, b) animal diagnosis and the treatment of dogs, and c) evaluation of extent of knowledge and performance of educational interventions among rural families and health, livestock, and education professionals and technicians, in order to help control the disease transmission cycle. Indirect hemagglutination and ELISA tests were performed on 5,556 apparently healthy people. Of these, 42 (0.8%) had positive results on both tests, for a seroprevalence of 754.6 per 100,000. These 42 subjects were scheduled for liver ultrasonography and a chest x-ray; of the 26 who complied, 16 showed images compatible with a hydatid cyst. Those 16 cases were sent to the hospital for surgery. In 9 of the cases the diagnosis was confirmed surgically, for a prevalence of 161.7 per 100,000. Arecoline hydrobromide was administered as a laxative to 2,358 dogs to detect the strobilar form of Echinococcus granulosus, and positive results were found in 11% of the dogs. Official data for slaughterhouses indicated the presence of hydatid cysts in 13% of the cattle, 4.4% of the sleep, and 4.2% of the pigs slaughtered in the region. The educational program included an evaluation of the extent of knowledge by surveying heads of household; an educational intervention among families through an informal active participatory process using educational games, in which 1,082 families participated; and an educational intervention with professionals and technicians using distance and in-person approaches. To evaluate the program, the results of knowledge tests before and after educational interventions with 200 families (cases) were compared with those from 95 families who did not participate (controls). Of the 1,423 heads of household initially surveyed about their knowledge of echinococcosis/hydatidosis, 783 of them (55%) said they knew nothing about the infection. It was found that the participatory educational games were well adapted to the lifestyle of people from rural areas and made change possible. Training was provided to 276 health professionals, 201 technical assistants, and 453 rural teachers. The program reached 100% of the staff members of the area's rural primary health care services.

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Section: Discussionunclassified

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Equinococosis/hidatidosis en la VII Región de Chile: diagnóstico e intervención educativa

Apt¹,

Vasco²,

Galdamez³

et al. 2000

Rev Panam Salud Publica

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“…The ELISA technique was developed according to the method described by Coltorti et al (1990), with the corresponding modifications for the diagnosis of toxocariasis (Sommerfelt et al, 2001). The positive cut-off value was calculated using sera collected at day 0 from the control group.…”

Section: Elisamentioning

confidence: 99%

Toxocara canis infections in a pig model: immunological, haematological and blood biochemistry responses

et al. 2006

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The immunological, haematological and enzymatic responses to the inoculation in pigs of 100,000 embryonated eggs of Toxocara canis were studied. Fifteen females were inoculated and three remained as controls. Haematological values were analysed from day 7 p.i. until day 126 p.i. In the inoculated group, white blood cells were raised on day 14 p.i. and eosinophil values on days 7, 14, 21, 35 and 49 p.i. showing significant differences compared with controls (P < 0.05). Absolute eosinophil counts (per ml) presented two rises, the first on days 7, 14 and 21 p.i. and the second on days 35 and 49 p.i. Blood biochemistry was maintained within normal values. Serological examination by ELISA to determine antibody levels against Toxocara canis L2/L3 excretory-secretory (ES) antigens showed values higher than the positive cut-off (1:32) from day 7 p.i. and until the end of the study on day 126 p.i., presenting two peaks: one on day 28 p.i. and the second covering days 49 to 56 p.i. Western blots of sera of inoculated animals presented, from day 7 p.i., two polypeptide bands of 55 and 70 kDa MW and, from day 56 p.i., an additional band of 120 kDa MW, all of which persisted until the end of the study. Immunological responses were sustained over time. No direct correlation was observed between the rise in eosinophils and antibody titres. To validate the conclusions, more studies are required on the polypeptide bands.

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“…Previously, Mitchell et al (1977) suggested that phosphorylcholine may be produced by parasites to promote the production of low affinity antibodies. The presence of phosphorylcholine and antiphosphorylcholine antibodies in E. granulosus antigens and in sera from hydatid patients, respectively, has been reported (Shepherd & McManus 1987, Coltorti et al 1990, Sterla et al 1997.…”

Section: Introductionmentioning

confidence: 99%

Echinococcus granulosus human infection stimulates low avidity anticarbohydrate IgG2 and high avidity antipeptide IgG4 antibodies

Sterla

Sato

Nieto

1999

Parasite Immunology

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Total IgG and IgG subclasses recognizing carbohydrate and peptidic epitopes from native and periodate treated partially purified hydatid cyst fluid (ppHCFA) and protoscolex somatic antigens (PSA) were tested by ELISA in hydatid patients sera. Binding of the major cross-reactive antiphosphorylcholine antibodies was inhibited with free reagent. A predominant anticarbohydrate antibody response against ppHCFA and PSA is shown. Although the main contributing IgG subclass to the antipeptide response against both antigens was IgG4, IgG1 also significantly contributed to the anti-PSA peptidic epitopes response. Western blot showed that IgG1 antibodies strongly recognized in ppHCFA a periodate susceptible 38 kDa antigen. The IgG4 antibodies mainly recognized the periodate-resistant 12, 16 and 24 kDa antigens. In addition, IgG2 antibodies recognized three strongly periodate-susceptible broad bands (116, 55 and 24 kDa antigens). PSA-specific IgG1 and IgG4 antibodies showed similar patterns of antigen recognition as well as no significant reduction of reactivity after periodate treatment while the IgG2 antibody recognition was strongly affected by this treatment. Furthermore, IgG2 showed significantly lower avidities than IgG1 and IgG4 antibodies recognizing both antigens. In conclusion, hydatid patients showed an enhanced production of low avidity anticarbohydrate IgG2 as well as high avidity antipeptide IgG4 antibodies.

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Detección de portadores asintomáticos de quistes hidatídicos: aumento de la especificidad del ensayo inmunoenzimático

Cited by 14 publications

References 15 publications

Equinococosis/hidatidosis en la VII Región de Chile: diagnóstico e intervención educativa

Equinococosis/hidatidosis en la VII Región de Chile: diagnóstico e intervención educativa

Toxocara canis infections in a pig model: immunological, haematological and blood biochemistry responses

Echinococcus granulosus human infection stimulates low avidity anticarbohydrate IgG2 and high avidity antipeptide IgG4 antibodies

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