Estudo, ao microscópio óptico e eletrônico, do rim de caes natural e experimentalmente infectados com Leishmania (Leishmania) chagasi

Tafuri, Washington Luiz; Michalick, Marilena Suzan Marques; Dias, Magno; Genaro, Odair; Leite, Virgínia Hora Rios; Barbosa, Alfredo José Afonso; Bambirra, Eduardo Alves; Costa, Carlos Alberto da; Melo, Maria Norma; Mayrink, Wilson

doi:10.1590/s0036-46651989000300002

Cited by 26 publications

(13 citation statements)

References 7 publications

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“…Interstitial nephritis, characterized by the presence of lymphocytic infiltration, is a common finding in nephropathy due to dogs with leishmaniosis (TAFURI et al, 1989). According to Costa et al (2000), it may be due to the presence of parasite antigens found in the inflammatory infiltrate.…”

Section: Discussionmentioning

confidence: 99%

Hypertension and its correlation with renal lesions in dogs with leishmaniosis

Braga

Leite

Rosa

et al. 2015

Rev. Bras. Parasitol. Vet.

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To evaluate the prevalence of hypertension and its correlation with the severity of renal injury and proteinuria in dogs with leishmaniosis, sixty-six dogs were divided into two groups. Group 1 (G1) was composed of 54 dogs included in stage 1 of chronic kidney disease (CKD), and group 2 (G2) of twelve dogs in stages 2 and 3 of CKD. Prevalence of hypertension was 28.8%, comprising 22.2% of the dogs from G1 and 58.3% from G2 (P=0.011). The mean arterial blood pressure (BP) of dogs from G1 (135.7 ± 20.5) was lower than from G2 (170.0 ± 26.3) (P <0.001). Urine proteincreatinine ratio (UP/C) revealed values above 0.5 in 75.7% of the dogs, with 34% presenting hypertension. All dogs with hypertension had histopathological and laboratory evidence of glomerular disease. Although there was no statistically significant correlation between elevated BP and the severity of glomerular lesions (P=0.408), there was a statistically significant correlation between elevated BP and increased UP/C in the studied population (P=0.002). Thus, dogs with leishmaniosis and renal disease must be screened for the presence of hypertension so that treatment may be instituted as early as possible, in countries where treatment is allowed, to prevent the progression of renal damage.Keywords: Histopathology, kidney, Leishmania infantum chagasi, systolic blood pressure. ResumoPara avaliar a prevalência de hipertensão arterial e sua correlação com a severidade da lesão renal e proteinúria em cães com leishmaniose, 66 cães foram divididos em dois grupos. O grupo 1 (G1), composto por 54 cães em estágio 1 de doença renal crônica (DRC), e o grupo 2 (G2) por 12 cães em estágios 2 e 3 de DRC. A prevalência de hipertensão foi de 28,8%, compreendendo 22,2% dos cães de G1 e 58,3% dos cães de G2 (p = 0,011). A pressão arterial média (PA) de G1 (135,7 ± 20,5) foi inferior a de G2 (170,0 ± 26,3) (P <0,001). A relação proteína creatinina urinária (P/C U) foi maior que 0,5 em 75,7% dos cães, dos quais 34% possuíam hipertensão. Todos os cães com hipertensão apresentavam doença glomerular. Embora não tenha sido observada correlação estatisticamente significativa entre elevação da PA e severidade das lesões glomerulares (P =0,408), houve uma correlação significativa entre PA elevada e aumento da UP/C (P = 0,002). Portanto, cães com leishmaniose e doença renal devem ser pesquisados quanto à presença de hipertensão, para que o tratamento possa ser instituído o mais precocemente possível em países onde ele é permitido, para evitar a progressão da lesão renal.Palavras-chave: Histopatologia, rim, Leishmania infantum chagasi, pressão arterial sistólica.

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Section: Discussionmentioning

confidence: 99%

Hypertension and its correlation with renal lesions in dogs with leishmaniosis

Braga

Leite

Rosa

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Some authors have suggested the involvement of immune complex deposits in the vascular endothelium or interstitium generating chemotaxis and establishing a local inflammatory response mainly provoking vasculitis in many organs 8,17,22 .…”

Section: Discussionmentioning

confidence: 99%

Chronic interstitial pneumonitis in dogs naturally infected with Leishmania (Leishmania) chagasi: a histopathological and morphometric study

Gonçalves

Tafuri

Melo

et al. 2003

Rev. Inst. Med. trop. S. Paulo

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SUMMARYEighteen mongrel dogs of unknown age and naturally infected with Leishmania (Leishmania) chagasi, were obtained from the City Hall of Belo Horizonte, Brazil. Four dogs were used as control. Lung samples were obtained and immediately fixed in formalin. The histopathological picture of all lung tissue sections was a chronic and diffuse interstitial pneumonitis. The thickened interalveolar septa were characterized by the cellular exudate (mostly macrophages, lymphocytes and plasmocytes) associated with collagen deposition. Morphometric analysis showed greater septal thickness in the infected animals than in controls. In fact, the morphometric study of collagen stained with ammoniac silver confirmed a larger deposition of collagen in the infected animals. The parasitologic method was carried out during the study of the lesions on the slides. However, we did not observe any correlation between the histopathologic and morphometric data and the clinical status of the animals. We conclude that the pulmonary lesions observed in all naturally infected dogs were correlated with the disease and that the morphometric method used was satisfactory for the analysis of septal thickness and of increased collagen deposition, confirming the presence of fibrosis.

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“…CVL infection is considerably more prevalent than clinical illness in endemic areas; this has been reported both in Brazil and Europe (Mediterranean basin countries) [7-13]. Furthermore, clinical signs of the disease are highly variable and can include lymphadenopathy [14,15], skin lesions [16-18], progressive weight loss [19], hepatosplenomegaly [20,21], ocular and musculoskeletal abnormalities, renal disease and epistaxis [22-24]. …”

Section: Introductionmentioning

confidence: 99%

Histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum

et al. 2011

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BackgroundThe aim of this study was to provide a systematic pathological and parasitological overview of the gastrointestinal tract (GIT), including the stomach, duodenum, jejunum, ileum, caecum and colon, of dogs naturally infected with Leishmania.MethodsTwenty mongrel dogs naturally infected with Leishmania (Leishmania) infantum and obtained from the Control Zoonosis Center of the Municipality of Ribeirão das Neves, Belo Horizonte Metropolitan area, Minas Gerais (MG) state, Brazil, were analyzed. The dogs were divided into two groups: Group 1 comprised nine clinically normal dogs and group 2 comprised 11 clinically affected dogs. After necropsy, one sample was collected from each GIT segment, namely the stomach, duodenum, jejunum, ileum, caecum and colon. Furthermore, paraffin-embedded samples were used for histological and parasitological (immunohistochemistry) evaluation and a morphometrical study were carried out to determine the parasite load (immunolabeled amastigote forms of Leishmania). The Friedman and the Mann Whitney tests were used for statistical analysis. The Friedman test was used to analyze each segment of the GIT within each group of dogs and the Mann Whitney test was used to compare the GIT segments between clinically unaffected and affected dogs.ResultsThe infected dogs had an increased number of macrophages, plasma cells and lymphocytes, but lesions were generally mild. Parasite distribution in the GIT was evident in all intestinal segments and layers of the intestinal wall (mucosal, muscular and submucosal) irrespective of the clinical status of the dogs. However, the parasite load was statistically higher in the caecum and colon than in other segments of the GIT.ConclusionThe high parasite burden evident throughout the GIT mucosa with only mild pathological alterations led us to consider whether Leishmania gains an advantage from the intestinal immunoregulatory response (immunological tolerance).

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Estudo, ao microscópio óptico e eletrônico, do rim de caes natural e experimentalmente infectados com Leishmania (Leishmania) chagasi

Cited by 26 publications

References 7 publications

Hypertension and its correlation with renal lesions in dogs with leishmaniosis

Hypertension and its correlation with renal lesions in dogs with leishmaniosis

Chronic interstitial pneumonitis in dogs naturally infected with Leishmania (Leishmania) chagasi: a histopathological and morphometric study

Histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum

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