1988
DOI: 10.1590/s0036-46651988000200008
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Abstract: Foi avaliado o teste imunoenzimático (ELISA), para detecção de anticorpos da classe IgM na leptospirose humana. Nas amostras de soros analisadas, o teste ELISA demonstrou ser mais sensível, específico e precoce, quando comparado ao teste de soroaglutinação microscópica. A análise dos resultados obtidos nesta avaliação demonstra que o teste ELISA permite detectar níveis baixos de anticorpos circulantes, e também anticorpos não aglutinantes. não detectáveis através do teste de soroaglutinação microscópica.

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Cited by 12 publications
(3 citation statements)
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“…1992), the antigen utilized in IgM ELISA was prepared according to the method described by Adler et al. (1980) modified by Silva et al. (1988) employing suspensions of five Leptospira interrogans serovars: Icterohaemorrhagiae, Canicola, Hebdomadis, Brasiliensis, and Cynopteri.…”
Section: Methodsmentioning
confidence: 99%
“…1992), the antigen utilized in IgM ELISA was prepared according to the method described by Adler et al. (1980) modified by Silva et al. (1988) employing suspensions of five Leptospira interrogans serovars: Icterohaemorrhagiae, Canicola, Hebdomadis, Brasiliensis, and Cynopteri.…”
Section: Methodsmentioning
confidence: 99%
“…The importance of IgA for mucosal host immunity is well established; however, knowledge about its protective role in systemic circulation is still doubtful . Nonetheless, in the last few years there has been increasing evidence of its usefulness for diagnosis of infectious diseases, such as leptospirosis, toxoplasmosis, and dengue . The IgA isotype presents an advantage in comparison to the IgM and IgG isotypes because it appears early in infection and disappears much faster than them .…”
Section: Introductionmentioning
confidence: 99%
“…ELISA-IgM. The ELISA-IgM was performed by the method described by Adler et al (1), with some modifications (12). The technique was carried out as described by Camargo et al (3).…”
mentioning
confidence: 99%