1986
DOI: 10.1590/s0036-46651986000400001
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Abstract: SUMMARYThe infectivity amastigotes of Trypanosoma cruzi, isolated from the supernatant of the J774G8 macrophage-like cell line infected with trypomastigotes to normal macrophages in vitro was tested. After a period of 1 h of T. cruzi-macro¬ phage interaction about 2% of the mouse peritoneal macrophages had ingested amastigotes. In contrast 12% of the macrophages had ingested epimastigotes. Treatment of the amastigotes with trypsin did not interfere with their ingestion by macrophages. Once inside the macrophag… Show more

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Cited by 22 publications
(16 citation statements)
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“…Whether these forms are destined to destruction or play a role in the parasite life cycle is not known 6 . There is also evidence that amastigotes are infective to mice, both in vitro and in vivo 23,53 , having already been suggested that they play an important part in the maintenance of the infection in vivo 70 . It is not known the relative importance of the two stages (trypomastigotes and amastigotes) in perpetuating the infection leading to the chronic phase of the disease 53 .…”
Section: Discussionmentioning
confidence: 99%
“…Whether these forms are destined to destruction or play a role in the parasite life cycle is not known 6 . There is also evidence that amastigotes are infective to mice, both in vitro and in vivo 23,53 , having already been suggested that they play an important part in the maintenance of the infection in vivo 70 . It is not known the relative importance of the two stages (trypomastigotes and amastigotes) in perpetuating the infection leading to the chronic phase of the disease 53 .…”
Section: Discussionmentioning
confidence: 99%
“…The fate of amastigotes at that time was thought to be restricted to the intracellular growth of the parasite. However, pioneering studies (Behbehani, 1973; Nogueira and Cohn, 1976; Lanar, 1979; Abrahamsohn et al, 1983; McCabe et al, 1984; Carvalho and de Souza, 1986) demonstrated that amastigotes shared some physiological characteristics with trypomastigotes, such as the ability to invade and develop within cells in vitro and the ability to infect mice. For instance, McCabe et al (1984) demonstrated that amastigotes isolated from the spleen of mice infected with three different strains of T. cruzi (Y, MR, and Tulahuén) were able to infect, survive, and replicate within professional and non-professional (L929 cell line) phagocytes.…”
Section: Mammalian Cell Invasion By Extracellular Amastigotes: the Stmentioning
confidence: 99%
“…The mouse macrophage cell line J774.G8 was obtained from the laboratory of W. De Souza (De Carvalho and De Souza, 1986;De Carvalho and De Souza, 1983) and is derived from the original J774.A1 from the American Type Culture Collection (ATCC, Rockville, MD). This cell line was grown in complete RPMI 1640 medium (Sigma Chemical Company, St Louis, MO) supplemented with 10% fetal calf serum (GIBCO-Life Technologies, Rockville, MD), 2 g l -1 NaHCO3, 100 U ml -1 penicillin and 100 µg ml -1 streptomycin, pH 7.4, on glass cover slips, 35 mm plastic and 25 cm 2 plastic culture flasks (Corning, USA) at 37°C in a 5% CO2 humidified atmosphere.…”
Section: Cell Culturementioning
confidence: 99%