Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

Yoshida, Nobuko; Teixeira, Marta Maria Geraldes; Sbravate, Clizete

doi:10.1590/s0036-46651986000200003

Cited by 3 publications

(1 citation statement)

References 12 publications

(5 reference statements)

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“…The parasites were grown at 28°C in liver infusion tryptose medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% inactivated fetal bovine serum (FBS) (LCG Biotecnologia, Cotia, SP, Brazil). Metacyclic forms of growing cultures were purified on a DEAE cellulose column, as described previously (Yoshida et al ., 1986).…”

Section: Methodsmentioning

confidence: 99%

Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

et al. 2021

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Congenital transmission of Chagas disease plays an important role in endemic countries because it is not a diagnosis that is encountered frequently in prenatal care. Due to limited information regarding congenital transmission of Trypanosoma cruzi in Mexico, the present study aimed to investigate protozoan infectivity and modulation of immune responses in human placental explants infected with T. cruzi Ia Mexican strains. The Inc-5 strain showed increased infectivity and modulated IL-1β, IL-10 and TLR-4, decreasing their expression after 24 h of infection. Both strains (Inc-5 and Ninoa) stimulated the production of TNF-α and decreased IL-6 levels 96 h after infection. An important detachment of the syncytiotrophoblast caused by infection with T. cruzi was observed after 24 h of infection. In this study, ex vivo infection of human placental villi was performed to better understand interactions involving parasitic T. cruzi and human placental tissue. It was concluded that the strains of TcIa present parasitism in placental tissue, modulation of the innate immune system of the placenta, and cause intense detachment of the syncytiotrophoblast, a fact that may be more associated with abortion and premature birth events than the congenital transmission itself, justifying the low rate of this transmission mechanism by this genotype.

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Section: Methodsmentioning

confidence: 99%

Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

et al. 2021

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Rapid isolation of metacyclic trypomastigotes of Trypanosoma cruzi from feces and urine of the vector

Schaub

1991

Acta Tropica

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Immunization and challenge of mice with insect‐derived metacyclic trypomastigotes of Trypanosoma cruzi

Kirchhoff

Hoft

1990

Parasite Immunology

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Infection with Trypanosoma cruzi was established in the reduviid vector, Dipetalogaster maximus, by repeated feedings on mice with high parasitaemias, and metacyclic trypomastigotes (IMT) were collected in insect urine after blood meals. The infectivity of IMT in mice was assessed by placing varying numbers of organisms, ranging from 5 to 5000, on to the conjunctivae or oral mucosa of anaesthetized animals. Half of the mice exposed to as few as 20 IMT by either route became parasitized, and the minimum inoculum size that resulted in all mice becoming infected was 640 IMT by the ocular route and 1250 IMT in the mice given parasites by mouth. Mice were immunized by tail vein infusion of irradiated IMT. Animals in the immunized group and in two control groups were then challenged by deposition of IMT on to the oral mucosa. Two of five immunized mice and nine of 10 comparison animals developed acute T. cruzi infection after challenge. These results indicate that IMT produced in this system are highly infective and that inocula containing 500-1000 IMT applied to the conjunctivae or oral mucosa constitute a gentle contaminative challenge. Moreover, our findings suggest that sterile protection against a contaminative challenge may be inducible by immunization with IMT, but experiments involving larger numbers of animals must be performed to resolve this question.

show abstract

Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

Cited by 3 publications

References 12 publications

Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

Rapid isolation of metacyclic trypomastigotes of Trypanosoma cruzi from feces and urine of the vector

Immunization and challenge of mice with insect‐derived metacyclic trypomastigotes of Trypanosoma cruzi

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