“…In 1979, Yoshida et al, published a preliminary note recording the isolation of a Leishmania species from pooled liver and spleen of an opossum, Didelphis marsupialis aurita, captured in forest in Conchas, SP, Brazil and, in a later communication (Yoshida et al 1985), considered the parasite to be a member of the L. mexicana complex. Barretto et al (1985) made an isolation of a similar parasite from the skin of a single specimen of the rodent Proechimys iheringi denigratus caught in forest in the municipality of Três Braços, State of Bahia, Brazil, and again referred to it as L. mexicana.…”