Caffeine effect on mortality and oviposition in successive generations of Aedes aegypti

Abstract

OBJECTIVE:Previous experiments showed that caffeine blocks the development of Aedes aegypti (Diptera, Culicidae) in the larval stage, consequently inhibiting the production of adults. This study aimed at obtaining data suggestive of caffeine resistance by these mosquitoes. METHODS:Experiments were carried out in successive generations to assess adult production from eggs laid in previous generation and oviposition rate in every generation using 200 and 500 µg/mL caffeine. Tap water was used as control. Experiments were conducted in the city of São José do Rio Preto, Southeastern Brazil between 2002 and 2005. Statistical tests consisted of exploratory data analysis and smoothing algorithms. RESULTS:Increasing reduction in productivity of adults occurred among generations at both caffeine concentrations but the differences were only significant at 200µg/mL caffeine. As for the oviposition rate, there was a decrease in the mean number of eggs per female over generations at both caffeine concentrations. CONCLUSIONS:There was no evidence of caffeine resistance over generations. The study results corroborate caffeine as an alternative as an important Ae. Aegypti control agent to avoid resistance. KEYWORDS: Aedes RESUMOOBJETIVO: Experimentos anteriores mostraram que a cafeína bloqueia o desenvolvimento de Aedes aegypti (Diptera, Culicidae) na fase larval, inibindo conseqüentemente a produção de adultos. O objetivo do estudo foi obter dados que pudessem sugerir desenvolvimento de resistência dos mosquitos à cafeína. MÉTODOS:Foi avaliada a produção de adultos em gerações sucessivas, a partir de ovos produzidos na geração anterior e a taxa de oviposição em cada geração, utilizando meios contendo cafeína a 200 e 500 µg/ml e água de torneira proveniente de poço artesiano como controle.

Caffeine effects on AdoR mRNA expression in Drosophila melanogaster

“…The concentrations used in the present study caused effects that are in agreement with the data from other reports investigating the effects of caffeine on insect development [25,30,35]. Exposure to caffeine in concentrations of up to 1 mg · mL -1 slows development and causes moderate toxic effects, whereas higher ones are much more toxic.…”

“…In the conducted experiments, caffeine was indeed very effective in disturbing the development of D. melanogaster. A significant decrease of the larva to imago rate and an increased time of development was observed, which is consistent with the literature data [21,29,30]. Currently, the AdoR antagonism is perceived as the main route of the action of caffeine [13,31] and only one adenosine receptor isoform (sequence) is known in D. melanogaster.…”

“…To the best of our knowledge, such a phenomenon has never been described before. Another explanation may be that AdoR expression in the imago stage is fixed [35] or changes can only be observed in the protein density, which would be the same as in case of the A1 receptor in rats [38]. There are no other data about the AdoR level or mRNA in the imago body after caffeine treatment of larvae.…”

Effects of Ibuprofen and Caffeine Concentrations on the Common Bed Bug (Cimex lectulatius L.) Feeding and Fecundity

“…Similar observations were recorded in other insect species; the checker-spot butterfly (Euphydryas editha) had increased egg production and egg mass with increased consumption of carbohydrates and nectar [44]. In previous studies, [45,46] consumption of caffeine was shown to cause mortality in insects. Although not tested in this study, further research is needed to confirm mortality in bed bugs.…”

Selection of active plant extracts against the coffee leaf miner Leucoptera coffeella (Lepidoptera: Lyonetiidae)

“…(Alexander et al, 1991;Dutta et al, 1993;Franzios et al, 1997;Adedire & Lajide, 1999;Larocque et al, 1999;Botha & Mccrindle, 2000 Perhaps the results were different because the plants show variation in the production of secondary metabolites, according to the climatic conditions in which they were grown (Gobbo-Neto & Lopes, 2007). Moreover, different species of insects have different mechanisms for detoxification against the same substance, caffeine, for example, blocks the development of Aedes aegypti (Diptera: Culicidae), but does not have an adverse effect on Perileucoptera :coffeella (Lepidoptera: Lyonetiidae) (Guerreiro Filho & Mazzafera, 2000;Laranja et al, 2006). One must also consider the conditions of extraction, given the fact that secondary plant metabolites include several classes of substances.…”