Blue rayon e teste Salmonella/microssoma na avaliação da qualidade de águas costeiras

Kummrow, Fábio; Magalhães, Dulce; Franco, Alexandre; Umbuzeiro, Gisela de Aragão

doi:10.1590/s0034-89102006000600020

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…Estruturas químicas dos oito PBTA já identificados têm muitos toxicantes ainda não identificados e não regulados que podem ser carcinogênicos e, assim, representar um risco à saúde humana de magnitude desconhecida 9 . A utilização de técnicas seletivas de extração/concentração de amostras, como o blue rayon, associadas a diferentes linhagens de Salmonella typhymurium do teste de Salmonella/microssoma podem sugerir as principais classes de contaminantes genotóxicos presentes no ambiente avaliado [10][11][12] .…”

Section: Presença Dos Pbta No Meio Ambienteunclassified

2-fenilbenzotriazóis (PBTA): uma nova classe de contaminantes ambientais

Kummrow

Umbuzeiro²

2008

Quím. Nova

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A NEW CLASS OF ENVIRONMENTAL CONTAMINANTS. This work is a literature review of PBTAs, the phenylbenzotriazoles generated from the reduction and chlorination of azo dyes. The PBTAs and the nonchlorinated PBTAs were isolated for the first time from river blue rayon organic extracts that showed high mutagenic activity in the Salmonella/microsome genotoxicity assay. To date, 8 PBTAs have been identified and beside their mutagenic activity in bacteria they cause genotoxic effects in fish and mammalian cell cultures. Due to the large number of textile dyeing facilities in Brazil, studies to determine them in the aquatic environment seem to be relevant.

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Section: Presença Dos Pbta No Meio Ambienteunclassified

2-fenilbenzotriazóis (PBTA): uma nova classe de contaminantes ambientais

Kummrow

Umbuzeiro²

2008

Quím. Nova

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Mutagenicity of blue rayon extracts of fish bile as a biomarker in a field study

Santos

Kummrow

Albergaria-Barbosa

et al. 2009

Environ and Mol Mutagen

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Blue rayon (BR) in combination with the Salmonella/microsome assay was used to evaluate the mutagenicity of fish bile samples. Specimens of Mugil curema from two sites were collected over a 1-year period. Piaçaguera channel contains high concentrations of total polycyclic aromatic hydrocarbons (PAHs) and other contaminants, while Bertioga channel was considered the reference sites in this study. Bile was extracted with BR and tested with TA98, TA100, and YG1041 strains with and without S9 in dose response experiments. PAH metabolite equivalents were analyzed using reverse-phase high performance liquid chromatography /fluorescence. Higher mutagenic responses were observed for the contaminated site; YG1041 with S9 was the most sensitive strain/condition. Mutagenicity ranged from 3,900 to 14,000 rev./mg at the contaminated site and from 1,200 to 2,500 rev./mg of BR at the reference site. The responses of YG1041 were much higher in comparison with the TA98 indicating the presence of polycyclic compounds from the aromatic amine class that cause frameshift mutation. TA100 showed a positive mutagenic response that was enhanced following S9 treatment at both sites suggesting the presence of polycyclic compounds that require metabolic activation. benzo(a)pyrene, naphthalene, and phenanthrene metabolite equivalents were also higher in the bile of fish collected at the contaminated site. It was not possible to correlate the PAH metabolite quantities with the mutagenic potency. Thus, a combination of the Salmonella/microsome assay with YG1041 with S9 from BR bile extract seems to be an acceptable biomarker for monitoring the exposure of fish to mutagenic polycyclic compounds.

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Design, Biological Evaluation, and Computer-Aided Analysis of Dihydrothiazepines as Selective Antichlamydial Agents

et al. 2023

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Chlamydia trachomatis (CT) causes the most prevalent sexually transmitted bacterial disease in the United States. The lack of drug selectivity is one of the main challenges of the current antichlamydial pharmacotherapy. The metabolic needs of CT are controlled, among others, by cylindrical proteases and their chaperones (e.g., ClpX). It has been shown that dihydrothiazepines can disrupt CT-ClpXP. Based on this precedent, we synthesized a dihydrothiazepine library and characterized its antichlamydial activity using a modified semi-high-throughput screening assay. Then, we demonstrated their ability to inhibit ClpX ATPase activity in vitro, supporting ClpX as a target. Further, our lead compound displayed a promising selectivity profile against CT, acceptable cytotoxicity, no mutagenic potential, and good in vitro stability. A two-dimensional quantitative structure−activity relationship (2D QSAR) model was generated as a support tool in the identification of more potent antichlamydial molecules. This study suggests dihydrothiazepines are a promising starting point for the development of new and selective antichlamydial drugs.

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Blue rayon e teste Salmonella/microssoma na avaliação da qualidade de águas costeiras

Cited by 4 publications

References 21 publications

2-fenilbenzotriazóis (PBTA): uma nova classe de contaminantes ambientais

2-fenilbenzotriazóis (PBTA): uma nova classe de contaminantes ambientais

Mutagenicity of blue rayon extracts of fish bile as a biomarker in a field study

Design, Biological Evaluation, and Computer-Aided Analysis of Dihydrothiazepines as Selective Antichlamydial Agents

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