Aislamiento de especies termotolerantes de Campylobacter en dos poblaciones de pollos criados con y sin confinamiento

Tresierra-Ayala, Álvaro; Fernández, Heriberto; Bendayán, María; Pereyra, Gustavo; Bernuy, Alfonso

doi:10.1590/s0034-89101995000500008

Cited by 13 publications

(6 citation statements)

References 14 publications

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“…From Brazil and Costa Rica, 100.0% respectively 80.0% of the flocks were reported to be positive for Campylobacter when ceca samples were studied (Giombelli and Gloria, 2014 ; Zumbaco-Gutiérrez et al, 2014 ). On the other hand, in Argentina and Chile Campylobacter was found in 33.3% of samples (Rivera et al, 2011 ; Zbrun et al, 2013a ) while in Peru Tresierra-Ayala et al ( 1995 ) reported a prevalence of 35%. Other tropical countries such as Vietnam and South Africa have reported a prevalence of 31.9 and 14.2% respectively (Jonker and Picard, 2010 ; Carrique-Mas et al, 2014 ).…”

Section: Discussionmentioning

confidence: 98%

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Vinueza-Burgos

Wautier²,

Martiny

et al. 2017

Poultry Science

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Thermotolerant Campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. The linkage of human campylobacteriosis and poultry has been widely described. In this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of C. coli and C. jejuni in broilers from Ecuador. Caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of Pichincha during 1 year. Microbiological isolation was performed by direct plating on mCCDA agar. Identification of Campylobacter species was done by PCR. Minimum inhibitory concentration (MIC) values for gentamicin, ciprofloxacin, nalidixic acid, tetracycline, streptomycin, and erythromycin were obtained. Genetic variation was assessed by RFLP-flaA typing and Multilocus Sequence Typing (MLST) of selected isolates. Prevalence at batch level was 64.1%. Of the positive batches 68.7% were positive for C. coli, 18.9% for C. jejuni, and 12.4% for C. coli and C. jejuni. Resistance rates above 67% were shown for tetracycline, ciprofloxacin, and nalidixic acid. The resistance pattern tetracycline, ciprofloxin, and nalidixic acid was the dominant one in both Campylobacter species. RFLP-flaA typing analysis showed that C. coli and C. jejuni strains belonged to 38 and 26 profiles respectively. On the other hand MLST typing revealed that C. coli except one strain belonged to CC-828, while C. jejuni except 2 strains belonged to 12 assigned clonal complexes (CCs). Furthermore 4 new sequence types (STs) for both species were described, whereby 2 new STs for C. coli were based on new allele sequences. Further research is necessary to estimate the impact of the slaughter of Campylobacter positive broiler batches on the contamination level of carcasses in slaughterhouses and at retail in Ecuador.

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Section: Discussionmentioning

confidence: 98%

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Vinueza-Burgos

Wautier²,

Martiny

et al. 2017

Poultry Science

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“…contamination level in properties that do not have Federal Veterinary inspection, however, the same it happens in places where it exists. Tresierra-Ayala et al (30) reported similar contamination levels when studying samples from family producers (54%) as well as commercial poultry farms (35%) with Federal Veterinary inspection.…”

Section: Resultsmentioning

confidence: 73%

“…Other authors (4,16,29-31) have reported higher percentages, as well as different thermophilic Campylobacter species. In a similar work, Tresierra-Ayala et al (30) isolated C. jejuni in 27% of the fecal samples of free range chickens, belonging to families of low social-economic level (Iquitos city, Peru). In another study, the same authors reported the presence of C. coli (17%) and C. lari (10%) in fecal samples of domestic chicken (29).…”

Section: Resultsmentioning

confidence: 90%

“…C. jejuni and C. coli are the most important agents that cause human enteritis and less frequently C. lari (28). This is a food-borne infection and the consumption of undercooked chicken meat has been identified as a significant risk factor (2,30). However, other factors, such as non-pasteurized milk (7,24) and contaminated water consumption (24,25), as well as contact with small and farm animals (2,24) have also been reported as sources of infection (26).…”

Section: Introductionmentioning

confidence: 99%

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Campylobacter jejuni occurrence in chicken fecal samples from small properties in Pelotas, southern of Brazil

Gomes¹,

Curcio²,

Ladeira³

et al. 2006

Braz. J. Microbiol.

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The aim of this study was to evaluate the occurrence of thermophilic Campylobacter species in broiler flocks from 26 small properties located near the campus of Federal University of Pelotas, RS, Brazil. A total of 404 chicken fecal samples were analyzed and after isolation, identification and biotyping was performed according to Lior´s scheme. Twenty one strains (5.2%) of Campylobacter jejuni biotype II were isolated from animals from seven properties (26.9%), with a variation from 3% to 34.6% among the positive properties. We also analyzed twelve C. jejuni isolates by polymerase chain reaction (PCR), using specific primers that generate a fragment of 402 pb. Our study allowed us to observe that chickens raised in non-industrial establishments without sanitary attention can harbour C. jejuni in their intestinal tract, and by doing so, they could be a serious health risk to humans.

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“…Respecto al tipo de crianza de las aves, se ha observado una mayor prevalencia de Campylobacter sp. en crianza doméstica, respecto a aves de poblaciones confinadas 29 .…”

Section: Discussionunclassified

Caracterización de cepas de Campylobacter jejuni obtenidas desde carne de pollo y heces de aves de corral de la zona central de Chile

et al. 2017

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Background Campylobacter jejuni is one of the main causal agents of food borne diseases. Infections with this pathogen are mainly caused by chicken meat consumption. Aim To characterize antibiotic resistance and virulence factors in C. jejuni strains obtained from chicken meat and poultry feces in Central Chile. Material and Methods The presence of C. jejuni in 30 meat and 40 feces samples from poultry was studied. From these samples, we obtained 40 strains which were characterized at the molecular level for the presence of 16 genes involved in virulence using PCR. In parallel, antibiotic resistance for ciprofloxacin, nalidixic acid, tetracycline, erythromycin, azithromycin, chloramphenicol y ampicillin was analyzed. Results Twenty and 63% of feces and chicken meat samples were positive for C. jejuni, respectively. Moreover, a high percentage of strains showed antibiotic resistance, where 27% of strains were resistant to all tested antibiotics, except for azithromycin. Finally, 10% of the strains coming from feces contained 14 out of 16 virulence genes evaluated. Only 23% of the strains did not contain any of these genes. Conclusions A high percentage of feces and chicken meat samples are contaminated with C. jejuni. Moreover, these strains show a high genetic and phenotypic diversity represented by their antibiotic resistance profiles and the presence of virulence factors.

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Aislamiento de especies termotolerantes de Campylobacter en dos poblaciones de pollos criados con y sin confinamiento

Cited by 13 publications

References 14 publications

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Campylobacter jejuni occurrence in chicken fecal samples from small properties in Pelotas, southern of Brazil

Caracterización de cepas de Campylobacter jejuni obtenidas desde carne de pollo y heces de aves de corral de la zona central de Chile

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