1992
DOI: 10.1590/s0034-89101992000100004
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Abstract: TIMENETSKY, J. et al. Identificação de micoplasmas pela inibição de crescimento de amostras isoladas de culturas celulares, Rev. Saúde públ., S.Paulo, 26 (1): 17 -20 , 1992.As culturas celulares devem ser continuamente monitoradas quanto à presença de micoplasmas, pois, embora às vezes eles passem despercebidos, podem causar alterações cromossômicas, interferir na replicação viral, na produção de anticorpos e interferon. A Organização Internacional em Micoplasmologia (IOM) recomenda o isolamento e a identifica… Show more

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Cited by 5 publications
(4 citation statements)
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“…They can cause chromosomal alterations, as well as changes in cellular metabolism and viral replication. According to the literature, the most common sources of contamination are oral human microbiota due to operator contamination, and bovine microbiota originating from sera (Timenetsky et al, 1992).…”
Section: Adventitious Agent Testingmentioning
confidence: 99%
“…They can cause chromosomal alterations, as well as changes in cellular metabolism and viral replication. According to the literature, the most common sources of contamination are oral human microbiota due to operator contamination, and bovine microbiota originating from sera (Timenetsky et al, 1992).…”
Section: Adventitious Agent Testingmentioning
confidence: 99%
“…The contamination through mycoplasma in bovine cells and sera used to produce vaccine antigens or used in diagnosis can generate non-confident results and add potentially noxious effects to biotechnological products. Contaminations through mycoplasma cause alterations in the cellular metabolism, decreasing the cellular division rate through the interference in DNA, RNA and protein synthesis, chromosomal aberrations and death with the monolayer release (Timenetsky et al 1992). Products, such as vaccines, medicines or monoclonal antibodies, manufactured based on such inputs should be disenabled, which causes prejudices to the pharmaceutical and biotechnological industries (Uphoff and Drexler 2002).…”
Section: Introductionmentioning
confidence: 99%
“…The tests to detect the contamination by mycoplasma are necessary to assure the confidence of the results and the quality of the biotechnological products (Uphoff and Drexler 2002;Timenetsky et al 2006). As this kind of contaminant does not generate visual signals, because they do not cause turbidity, just specific tests enable its detection (Lincoln and Lundin 1990;Timenetsky et al 1992;Hu et al 1995;Ossewaarde et al 1996). Among the different techniques to detect the contamination through mycoplasma, the histochemical staining, immunosorbent assays (ELISA), indirect immunofluorescence (IIFT), biochemical assays and polymerase chain reaction (PCR) are used (Kong et al 2001;Uphoff and Drexler 2002;Sung et al 2006;Gopalkrishna et al 2007).…”
Section: Introductionmentioning
confidence: 99%
“…Contaminations through mycoplasma cause alterations in the cellular metabolism, decreasing the cellular division rate through the interference in DNA and RNA synthesis, chromosomal aberrations and death with the monolayer release (Timenetsky et al , 1992). However, there are few studies that quantify which Mycoplasma concentration can affect such growth.…”
Section: Introductionmentioning
confidence: 99%