Mosaico do pimentão

Costa, Á. S.; Alves, Sebastião

doi:10.1590/s0006-87051950000300005

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“…e C. frutescens L.) em todo o mundo, incluindo seis vírus pertencentes ao gênero Potyvirus: Potato virus Y (PVY), Tobacco etch virus (TEV), Pepper mottle virus (PepMoV), Pepper veinal mottle virus (PVMV), Chilli veinal mottle virus (ChiVMV) e Pepper yellow mosaic virus (PepYMV) (Cook & Anderson, 1959;Brioso, 1996;Caranta & Palloix, 1996;Dogimont et al, 1996;Caranta et al, 1997;Inoue-Nagata et al, 2002). No Brasil, o PVY foi relatado pela primeira vez em 1941 em batata (Solanum tuberosum L.) (Nóbrega & Silberschmidt, 1944), e apenas a partir de 1950 TABELA 1 -Origem dos isolados virais utilizados neste estudo em pimentão, já causando sérios prejuízos (Costa & Alves, 1950). As perdas causadas por essa virose no campo levaram Nagai (1968) a iniciar um programa de melhoramento de pimentão visando à incorporação de genes de resistência ao mosaico causado pelo PVY, dando origem à série de cultivares "Agronômico".…”

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Identidade e propriedades de isolados de potyvírus provenientes de Capsicum spp.

et al. 2004

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Vinte isolados virais provenientes de Capsicum spp. foram coletados em Minas Gerais, São Paulo, Espírito Santo e Rio de Janeiro visando definir a etiologia dos mosaicos. Para a caracterização biológica realizou-se teste de gama de hospedeiros e inoculação em cultivares diferenciadoras de pimentão (Capsicum annuum). Dois isolados provenientes de batata (Solanum tuberosum) (PVY N-BR e PVY O-BR) foram utilizados como controles. Os resultados indicaram considerável grau de variabilidade biológica entre os isolados, embora todos tenham sido identificados preliminarmente como Potato virus Y (PVY). A reação das cultivares diferenciadoras classificou os isolados como patótipo 1 ou 1.2 de PVY. Anti-soros foram produzidos a partir de partículas virais purificadas de um isolado fraco e um forte. O uso desses anti-soros em ELISA indireto levou a resultados positivos contra os isolados testados. Os anti-soros reagiram também contra PVY N-BR e PVY O-BR, embora este último tenha apresentado reação mais fraca. Para caracterização molecular, seqüenciaram-se os genes da polimerase (NIb) e da proteína capsidial (cp), e da região 3' não-traduzida (3'NTR) de isolados biologicamente distintos. A análise filogenética confirmou a identidade de seis isolados como Pepper yellow mosaic virus (PepYMV), um potyvírus descrito recentemente infetando pimentão no Brasil. Esse resultado sugere que o PepYMV pode ser a espécie de potyvírus predominante em Capsicum spp. no Brasil. O fato de isolados de PepYMV apresentarem gama de hospedeiros semelhante à do PVY, e de os dois vírus apresentarem relacionamento sorológico, ressalta a utilidade da análise molecular para a classificação de potyvírus provenientes de Capsicum spp.

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Section: Introductionunclassified

Identidade e propriedades de isolados de potyvírus provenientes de Capsicum spp.

et al. 2004

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Sap‐transmissible Mosaic Diseases of Solanaceous Crops in Trinidad

Dale¹

1954

Annals of Applied Biology

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With Plate 10) Four sap-transmissible viruses were isolated from cultivated Solanaceae in Trinidad : ( I ) tobacco mosaic virus, from tobacco, tomato and sweet pepper; (2) cucumber mosaic virus, from tobacco and petunia; (3) 'pepper vein-banding virus', probably related to pepper mosaic viruses in Puerto Rico and Brazil, from peppers and tobacco; (4) ' egg-plant mosaic virus ', possibly related to the tobacco ring-spot virus, from egg-plant and tomato. Pepper vein-banding virus causes leaf-crinkling and vein-banding in Physalis floridana, petunia, various NicotMna spp. and most peppers; the Large Bell Hot pepper is killed; tomato and egg-plant are immune. Egg-plant mosaic virus produces mosaic, ring-spotting, or both, on different solanaceous species. It also gives local and systemic ring-spotting on Chenopodium hybridum and necrotic local lesions on the primary leaves of cowpea (var. Black-eye) ; cucumber is a symptomless carrier. Only cucumber mosaic virus was found naturally infecting non-solanaceoua hosts, cucumber and certain common wild The thermal inactivation point of pepper vein-banding virus is 62' C., its dilution end-point 2 x and itslongevityinvitro6 days at 25-30" C. ; corresponding values for egg-plant mosaic virus are 78" C., ~o-"and over3 weeks. Aphisgossypii transmits cucumber mosaic and pepper vein-banding, but not egg-plant mosaic, of which Epitrix sp. is an occasional vector. Tobacco mosaic, as elsewhere, probably has no regular insect vectors in Trinidad.plants. Sap-transmissible mosaic diseases of solanaceous crops 24'A second, widespread in egg-plants (Solanum melongena L.), also occasionally causes mosaic in adjacent tomatoes. Brief notes on these two viruses, which he called

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Pepper veinal mottle virus‐a new member of the potato virus Y group from peppers (Capsicum annuum L. and C. frutescens L.) in Ghana

Brunt¹,

Kenten

1971

Annals of Applied Biology

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Pepper veinal mottle virus (PVMV), a previously undescribed virus widespread in Capsicumannuum and C. frutescens in the Eastern Region of Ghana, is acquired and inoculated in 2 min feeding periods by aphids (Myxuspersicae and Aphisgossypii); it is transmissible by inoculation of sap to eleven of fifteen Solanaceae and to five of forty-six other species within three of seventeen other families. The virus was propagated in Nicotiana clevelandii and Petunia hybrida, and assayed in Chenopodium quinoa, C. amaranticolor and C. murale. Sap from Capsicum annuum was infective after dilution to I O -~ but not I O -~, after 10 min at 55 but not 60 "C, and after 7 but not 8 days at 25 "C. Lyophilized sap from P. hybrida was infective after 6 years in vacuo.Yields of 10-25 mg of virus per kg of leaf tissue were consistently obtained from P . hybrida or N . clevelandii by extracting systemically infected leaves in 0.5 M borate (pH 7.8) containing 0.2 % mercaptoethanol and chloroform, followed by repeated precipitation with 50 g polyethylene glycol (M.w. 6000) per 1, several cycles of differential centrifugation and centrifugation in sucrose density-gradient columns. Virus preparations had ultraviolet absorption spectra typical of a nucleoprotein containing c. 6 yo nuclei acid (A 2601280 = 1.25; A 260/246 = 1.27) and contained numerous unaggregated and unbroken filamentous particles c. 770 x 12 nm which sedimented as a single component with a sedimentation coefficient (s&~) of 155 S. PVMV contained RNA (moles yo: G = 24, A = 23, C = 27, U = 26), and a single protein species with a molecular weight of ~2 o o c -~~o o o daltons. PVMV was not serologically related to potato virus Y (three strains), or to twelve other morphologically similar viruses, and seems to be a distinct member of the potato virus Y group. A previously undescribed virus, which we call pepper veinal mottle virus (PVMV), is prevalent in Capsicum annuum L. and C. frutescens L. in the Eastern Region of Ghana. We here describe the main properties of PVMV, a new member of the potato 236 A. A. BRUNT AND R. H. KENTEN virus Y group (Brandes, I 964), and a purification procedure consistently yielding 10-25 mg of virus/kg of leaf.

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Mosaico do pimentão

Cited by 6 publications

References 0 publications

Identidade e propriedades de isolados de potyvírus provenientes de Capsicum spp.

Identidade e propriedades de isolados de potyvírus provenientes de Capsicum spp.

Sap‐transmissible Mosaic Diseases of Solanaceous Crops in Trinidad

Pepper veinal mottle virus‐a new member of the potato virus Y group from peppers (Capsicum annuum L. and C. frutescens L.) in Ghana

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