Enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, Southern Brazil

Vidal, Luíne Rosele Renaud; Almeida, Sérgio Monteiro de; Messias-Reason, Iara José de; Nogueira, Meri Bordignon; Debur, Maria do Carmo; Pessa, Luís Felipe Cavalli; Pereira, Luciane Aparecida; Rotta, Indianara; Takahashi, Gislene Reche de Almeida; Silveira, Clyete Santos da; Araújo, Josianne Maria Reimann; Raboni, Sônia Mara

doi:10.1590/s0004-282x2011000400013

Cited by 23 publications

(18 citation statements)

References 33 publications

(47 reference statements)

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“…EVs were the most detected as in other studies in Brazil [Mendoza et al, ; Vidal et al, ] and elsewhere [Dupuis et al, ] suggesting that EVs are the most common cause of infection in the CNS. Over 50% of infections were detected in adults.…”

Section: Discussionsupporting

confidence: 62%

Detection ofHerpesvirus,Enterovirus, andArbovirusinfection in patients with suspected central nervous system viral infection in the Western Brazilian Amazon

et al. 2014

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Acute infections of the central nervous system (CNS) can be caused by various pathogens. In this study, the presence of herpesviruses (HHV), enteroviruses (EVs), and arboviruses were investigated in CSF samples from 165 patients with suspected CNS viral infection through polymerase chain reaction (PCR) and reverse transcriptase PCR. The genomes of one or more viral agents were detected in 29.7% (49/165) of the CSF samples. EVs were predominant (16/49; 32.6%) followed by Epstein-Barr virus (EBV) (22.4%), Varicella-Zoster virus (VZV) (20.4%), Cytomegalovirus (CMV) (18.4%), herpes simplex virus (HSV-1) (4.1%), (HSV-2) (4.1%), and the arboviruses (14.3%). Four of the arboviruses were of dengue virus (DENV) and three of oropouche virus (OROV). The detection of different viruses in the CNS of patients with meningitis or encephalitis highlight the importance of maintaining an active laboratory monitoring diagnostics with rapid methodology of high sensitivity in areas of viral hyperendemicity that may assist in clinical decisions and in the choice of antiviral therapy.

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Section: Discussionsupporting

confidence: 62%

Detection ofHerpesvirus,Enterovirus, andArbovirusinfection in patients with suspected central nervous system viral infection in the Western Brazilian Amazon

et al. 2014

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“…A higher positivity was observed on the fourth or fifth day 1,17 . In our study, most CSF samples were collected on the second day (median 12 h) 18 , which could explain the high number of negative samples. Additionally, the elevated number of RBCs due to traumatic lumbar puncture in the CSF samples, as well as the presence of hemoglobin, probably had an inhibitory effect generating false-negative results.…”

Section: Discussionmentioning

confidence: 84%

Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR

Almeida¹,

Raboni

Nogueira

et al. 2016

Arq. Neuro-Psiquiatr.

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The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was higher in CSF samples without RBCs than in samples with RBCs: 13(26%) and 36(9.2%), p = 0.001. In the group with positive EV RT-PCR, the mean + SD CSF RBC was 37 ± 183 cell/mm3; the group with negative results had 580 + 2,890 cell/mm3 (p = 0.007). The acceptable upper limit for CSF RBCs that could not influence RT-PCR was 108 cells/mm3. CSF samples with negative results for EV RT-PCR have more erythrocytes.

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“…Forty-nine (10.6%) and 10 (2.17%) samples were positive for EVs and herpes viruses, respectively. 17 The detected viruses and the detection rates, also vary according to the population of the study, geographical region, demographic and clinical findings of the selected patient group and the test method used. In our study, samples sent from every region of the country were included, but 85% of the samples were sent from training and research hospitals in Ankara.…”

Section: Discussionmentioning

confidence: 99%

Role of Viral Agents in Childhood Central Nervous System Infections

Coşgun

Bayrakdar

Karademirtok

et al. 2019

J Pediatr Infect Dis

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Rapid laboratory diagnosis in acute viral infections in the central nervous system (CNS) is important for the direction of treatment. In this study, we aimed to evaluate viral agents detected by real-time polymerase chain reaction (PCR) in cerebrospinal fluid (CSF) samples sent to the national virology reference laboratory for the diagnosis of acute CNS viral infection in the 0 to 18 age group. The results of 5,255 CSF samples submitted to the laboratory between January 1, 2009, and February 14, 2018, were retrospectively reviewed. Test results of CSF samples tested with the multiplex real-time PCR panel (Fast Track Diagnostics/Viral Meningitis commercial kit, Luxembourg) kit were evaluated. PCR positivity was detected in 216 (4.11%) of the 5,255 CSF samples tested. The most common agents were enteroviruses 92 (42.6%) and herpes simplex virus-1 (HSV-1) 87 (40.2%) while varicella-zoster virus (VZV) 24 (11.1%), mumps virus 10 (4.62%), parechovirus 2 (0.92%), and herpes simplex virus-2 (HSV-2) 1 (0.46%) were less often identified. Considering the possibility that a wide variety of microorganisms may be agents of CNS infections, the performing of routine laboratory tests with multiple, fast, and reliable nucleic acid tests such as multiplex PCR provides a practical and effective approach. In addition to the identification of agents, retrospective studies of the incidence of agents will shed light on the clinical approaches in terms of the agents to be considered in the preliminary diagnosis. We consider that our results are valuable in terms of the high number of samples examined by the national virology reference laboratory over a 9-year period, and will contribute to clinicians' understanding of the epidemiology of meningitis in our country, and assist in identifying when and how to investigate patients for possible meningitis.

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Enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, Southern Brazil

Cited by 23 publications

References 33 publications

Detection ofHerpesvirus,Enterovirus, andArbovirusinfection in patients with suspected central nervous system viral infection in the Western Brazilian Amazon

Detection ofHerpesvirus,Enterovirus, andArbovirusinfection in patients with suspected central nervous system viral infection in the Western Brazilian Amazon

Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR

Role of Viral Agents in Childhood Central Nervous System Infections

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