Isolation of neurosphere-like bodies from an adult patient with refractory temporal lobe epilepsy

Azevedo-Pereira, Ricardo Luiz; Medei, Emiliano; Mendez-Otero, Rosália; Souza, Jorge Paes Barreto Marcondes de; Alves-Leon, Soniza Vieira

doi:10.1590/s0004-282x2010000600023

Cited by 4 publications

(5 citation statements)

References 8 publications

(13 reference statements)

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“…These cells proliferated as neurospheres in vitro and differentiated into neural and glial lineage in vivo. In keeping with our results, several investigations have shown that adult neural stem cells can proliferate and differentiate into various cell lineages, including astrocytes and neurons [ 26 – 28 ]. In addition to the reduction of cortical lesion volume and improvement of neurological function, our previous study revealed that the administration of rat NS/PCs seeded in PM led to increased cell survival and production of three main neural cell types, i.e., neurons, oligodendrocyte, and astrocytes [ 22 ].…”

Section: Discussionsupporting

confidence: 91%

“…Adult neural stem cells are promising alternatives to current cell replacement therapies for neurodegenerative disorders [45,46]. hNS/PCs obtained from epileptic brain tissues have been suggested as a favorable source of adult neural stem cells which can be used for the treatment of various neurological disorders [28]. Several studies have pointed to the proliferative and multipotent properties of hNS/PCs obtained from resected brain tissues of patients with medically refractory epilepsy [17,[26][27][28].…”

Section: Discussionmentioning

confidence: 99%

“…hNS/PCs obtained from epileptic brain tissues have been suggested as a favorable source of adult neural stem cells which can be used for the treatment of various neurological disorders [28]. Several studies have pointed to the proliferative and multipotent properties of hNS/PCs obtained from resected brain tissues of patients with medically refractory epilepsy [17,[26][27][28]. These hNS/PCs have the potential to differentiate into both neuronal and glial cells associated with both excitatory and inhibitory synaptic plasticity [47].…”

Section: Discussionmentioning

confidence: 99%

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Improvement of Rat Spinal Cord Injury Following Lentiviral Vector-Transduced Neural Stem/Progenitor Cells Derived from Human Epileptic Brain Tissue Transplantation with a Self-assembling Peptide Scaffold

et al. 2021

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Spinal cord injury (SCI) is a disabling neurological disorder that causes neural circuit dysfunction. Although various therapies have been applied to improve the neurological outcomes of SCI, little clinical progress has been achieved. Stem cell–based therapy aimed at restoring the lost cells and supporting micromilieu at the site of the injury has become a conceptually attractive option for tissue repair following SCI. Adult human neural stem/progenitor cells (hNS/PCs) were obtained from the epileptic human brain specimens. Induction of SCI was followed by the application of lentiviral vector-mediated green fluorescent protein–labeled hNS/PCs seeded in PuraMatrix peptide hydrogel (PM). The co-application of hNS/PCs and PM at the SCI injury site significantly enhanced cell survival and differentiation, reduced the lesion volume, and improved neurological functions compared to the control groups. Besides, the transplanted hNS/PCs seeded in PM revealed significantly higher migration abilities into the lesion site and the healthy host tissue as well as a greater differentiation into astrocytes and neurons in the vicinity of the lesion as well as in the host tissue. Our data suggest that the transplantation of hNS/PCs seeded in PM could be a promising approach to restore the damaged tissues and improve neurological functions after SCI.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of Rat Spinal Cord Injury Following Lentiviral Vector-Transduced Neural Stem/Progenitor Cells Derived from Human Epileptic Brain Tissue Transplantation with a Self-assembling Peptide Scaffold

et al. 2021

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“…Although prolonged moderate cerebral hypothermia has been reported to improve the outcome for HIE children, it still cannot eliminate the combined risk of disability and death . Neural stem cells (NSCs) possess multipotential and self‐renewal abilities and are well known with low immunogenicity , thus becoming a candidate for HIE therapy. However, studies focusing on the efficacy and molecular events of NSCs in HIE are still limited.…”

Section: Introductionmentioning

confidence: 99%

NF‐κB Signaling is Involved in the Effects of Intranasally Engrafted Human Neural Stem Cells on Neurofunctional Improvements in Neonatal Rat Hypoxic–Ischemic Encephalopathy

Liu

Zhao

et al. 2015

CNS Neurosci Ther

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“…NSPC can be isolated from the fetal brain or from one of the two neurogenic niches that persist in the adult brain: the subventricular zone of the lateral ventricles and the hippocampal subgranular zone [14][15][16]. Despite the evidence that transplanted fetal NSPC can functionally integrate into the brain of patients with Parkinson's disease [17], there are several obstacles to the use of NSPC from these two sources in clinical trials in stroke.…”

Section: Main Cell Types Used In Neurorestorative Cell Therapies For mentioning

confidence: 99%

The Rise of Cell Therapy Trials for Stroke: Review of Published and Registered Studies

Rosado-de-Castro

Pimentel‐Coelho

Fonseca

et al. 2013

Stem Cells and Development

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Stroke is the second leading cause of death and the third leading cause of disability worldwide. Approximately 16 million first-ever strokes occur each year, leading to nearly 6 million deaths. Nevertheless, currently, very few therapeutic options are available. Cell therapies have been applied successfully in different hematological diseases, and are currently being investigated for treating ischemic heart disease, with promising results. Recent preclinical studies have indicated that cell therapies may provide structural and functional benefits after stroke. However, the effects of these treatments are not yet fully understood and are the subject of continuing investigation. Meanwhile, different clinical trials for stroke, the majority of them small, nonrandomized, and uncontrolled, have been reported, and their results indicate that cell therapy seems safe and feasible in these conditions. In the last 2 years, the number of published and registered trials has dramatically increased. Here, we review the main findings available in the field, with emphasis on the clinical results. Moreover, we address some of the questions that have been raised to date, to improve future studies.

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Isolation of neurosphere-like bodies from an adult patient with refractory temporal lobe epilepsy

Cited by 4 publications

References 8 publications

Improvement of Rat Spinal Cord Injury Following Lentiviral Vector-Transduced Neural Stem/Progenitor Cells Derived from Human Epileptic Brain Tissue Transplantation with a Self-assembling Peptide Scaffold

Improvement of Rat Spinal Cord Injury Following Lentiviral Vector-Transduced Neural Stem/Progenitor Cells Derived from Human Epileptic Brain Tissue Transplantation with a Self-assembling Peptide Scaffold

NF‐κB Signaling is Involved in the Effects of Intranasally Engrafted Human Neural Stem Cells on Neurofunctional Improvements in Neonatal Rat Hypoxic–Ischemic Encephalopathy

The Rise of Cell Therapy Trials for Stroke: Review of Published and Registered Studies

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