Reibergrama para la evaluación de la síntesis intratecal de C3c

Abstract

RESUMEN -I n t roducción: El diagrama de las razones de Reiber o re i b e rgrama cobra cada día mayores usos para la caracterización de la síntesis intratecal de proteínas. El re i b e rgrama fue definido para las clases m a y o res de inmunoglobulinas pero luego ha sido utilizado para evaluar otras proteínas basado en la teoría de la difusión molecular/velocidad de flujo del líquido cefalorraquídeo (LCR). Método: El C3c, producto de la degradación del factor del complemento C3 y con una masa molecular de 145 KDa, se acerca a las características moleculares de la IgG para las leyes de la difusión de Fick. Se asume las constantes de la IgG en la fórmula de Reiber para evaluar la síntesis intratecal de C3c así como su correspondiente re i b e rg r a m a . Se estudiaron 27 pacientes y 27 controles a los que se les dosificó albúmina y C3c en suero y LCR por inmunodifusión radial. Resultados: Con el re i b e rgrama propuesto para el C3c se evaluaron estos pacientes. Se comprueba la validez de este re i b e rgrama para distintas condiciones de barrera con o sin síntesis intratecal de C3c. Conclusion: El re i b e rgrama y su fórmula correspondiente propuesto para la C3c puede ser usado para la evaluación de la síntesis intratecal de C3c.PALABRAS CLAVES: C3c, albúmina, inmunodifusión, nefelometría, reibergrama, síntesis intratecal. Reibergram for C3c intrathecal synthesis evaluationABSTRACT -I n t roduction: Reiber´s quotient diagram or re i b e rgram has a growing apply for characterize the intratecal synthesis of proteins. Firstly re i b e rgrama was used for the major classes of immunoglobulins but later it was used to evaluate other proteins based on the theory about molecular flux/cere b ro s p i n a l fluid (CSF) flow rate. Method: C3c is a degradation product of complement factor C3 with 145 KDa and a p p roaches to IgG molecular characteristics according with Fick´s diffussion laws. It was assumed IgG constants and graphic for IgG constants and graphic to evaluate the intrathecal synthesis of C3c. Twentyseven patients and 27 controls were studied. Serum and CSF C3c and albumin were quantified by i m m u n o d i ff u s i o n . Results: The patients with the C3c proposed re i b e rgram were evaluated. It has been p roved its validity under several CSF blood barrier conditions. Conclusion: R e i b e rgram for C3c can be used for the evaluation of the intrathecal synthesis of this protein.

C3c intrathecal synthesis evaluation in patients with multiple sclerosis

“…this last assertion means that the system has been activated and that we are in the presence of an immunological event associated to a type II or citotoxic hypersensibility. c3c intrathecal synthesis helps us to understand the physiopathological mechanisms when we are in the presence of infectious or autoimmune neurological diseases as Ms 11 .…”

Reibergram of Intrathecal Synthesis of C4 in Patients with Eosinophilic Meningitis Caused by Angiostrongylus cantonensis

“…Results were plotted on a CSF/serum quotient diagram (Reibergram) designed for C 4 , which was introduced for quantification of intrathecal synthesis of this component of the complement system and constructed on the same principle as quotient diagrams for CSF immunoglobulins. 13 This procedure is based on molecular diffusion/CSF flow theory, and its fundamental principle is that a decrease in the flow rate is always accompanied by an increase in molecular diffusion from blood to CSF. [14][15][16] The Reibergram ( Figure 1 ) was constructed by using the ratios Q albumin (CSF albumin/ serum albumin) plotted on the ordinate axis and the Q C 4 (CSF C 4 /serum C 4 ) plotted on the abscissa axis.…”

Intrathecal Activation as a Typical Immune Response within the Central Nervous System in Angiostrongyliasis

“…18,19 Complement component C3c is a degradation and stable product of C3; it is currently quantified instead of the whole C3 molecule because its presence indicates that C3 was biologically used in the immune response. The use of C3c and C4 Reibergrams has also been reported; 20,21 these are used to confirm that local complement factor was synthesized in the central nervous system (CNS). 20,21 The validity of this option is supported by the molecular flux/CSF flow theory.…”

“…The use of C3c and C4 Reibergrams has also been reported; 20,21 these are used to confirm that local complement factor was synthesized in the central nervous system (CNS). 20,21 The validity of this option is supported by the molecular flux/CSF flow theory. 22,23 Recently, different major immunoglobulin patterns in patients with angiostrongyliasis patients in Ecuador and Cuba with dissimilar neuroimmunologic and epidemiologic features, have been reported.…”

“…5,6 Other reports described intrathecal synthesis patterns of major immunoglobulins 24 and C3 and C4 in patients with angiostrongyliasis. 20,21 This report reviews the different patterns of this immune response and defines the concept of intrathecal activation of complement. It also aims to explain the relevance of these patterns during oligoclonal intrathecal synthesis, as well as the activation in CSF of one or more complement system components in meningoencephalitis caused by A. cantonensis.…”