COMPARISON OF SELECTIVE AND NON SELECTIVE CYCLO-OXYGENASE 2 INHIBITORS IN EXPERIMENTAL COLITIS EXACERBATION: role of leukotriene B4 and superoxide dismutase

Abstract

-Context -Nonsteroidal anti-inflammatory drugs are considered one of the most important causes of reactivation of inflammatory bowel disease. With regard to selective cyclo-oxygenase 2 inhibitors, the results are controversial in experimental colitis as well as in human studies. Objective -The aim this study is to compare nonsteroidal anti-inflammatory drugs effects, selective and non selective cyclo-oxygenase 2 inhibitors, in experimental colitis and contribute to the understanding of the mechanisms which nonsteroidal anti-inflammatory drugs provoke colitis exacerbation. Methods -Six groups of rats: without colitis, with colitis, and colitis treated with celecoxib, ketoprofen, indometacin or diclofenac. Survival rates, hemoglobin, plasmatic albumin, colonic tissue of interleukin-1ß, interleukin-6, tumor necrosis factor alpha, prostaglandin E2, catalase, superoxide dismutase, thiobarbituric acid-reactive substances, chemiluminescence induced by tert-butil hydroperoxides, and tissue and plasmatic leukotriene B4 were determined. Results -The groups treated with diclofenac or indometacin presented lower survival rates, hemoglobin and albumin, higher tissue and plasmatic leukotriene B4 and tissue superoxide dismutase than the group treated with celecoxib. Ketoprofen presented an intermediary behavior between diclofenac/indometacin and celecoxib, concerning to survival rate and albumin. The groups without colitis, with colitis and with colitis treated with celecoxib showed leukotriene B4 and superoxide dismutase lower levels than the groups treated with nonselective cyclo-oxygenase 2 inhibitors. Conclusions -Diclofenac and indometacin presented the highest degree of induced colitis exacerbation with nonsteroidal anti-inflammatory drugs, celecoxib did not show colitis exacerbation, and ketoprofen presented an intermediary behavior between diclofenac/indometacin and celecoxib. These results suggest that leukotriene B4 and superoxide dismutase can be involved in the exacerbation of experimental colitis by nonselective nonsteroidal anti-inflammatory drugs. HEADINGS -Colitis. Non-steroidal anti-inflammatory agents. Eicosanoids. Oxidative stress.

Comparative Evaluation of Aloe vera and Diclofenac on Body Weight, Blood Pressure and Renal Function of Hypertensive Rats

“…Cut of value for systolic hypertension was taken as 140 mm Hg. After induction of hypertension, rats in group B, C and D received 0.5 ml distilled water, 400 mg/kg Aloe vera gel powder 11 and 12 mg/kg diclofenac powder 12 respectively orally as a single morning dose for two weeks. Body weight of animals was recorded at baseline and then weekly throughout the study by Shimadzu ELB 3000 digital weighing balance.…”

Impact of anti-inflammatory agent like Bryophyllum pinnatum aqueous extract and diclofenac on blood pressure and creatinine clearance.

“…After that group B (positive control group) received distilled water 0.5 ml was by oral route, group C (Bryophyllum pinnatum group) received Bryophyllum pinnatum aqueous extract 300 mg/ml intraperitoneally 9 and group D received diclofenac 12 mg/kg intraperitoneally as a single morning dose for two weeks. 11 Blood pressure of animals was recorded at baseline and then weekly throughout the study. Five Systolic blood pressure readings were measured by tail cuff 12 using non-invasive blood pressure controller (ML125R) attached to computer based data recording system.…”

A Review of the Effects of Pain and Analgesia on Immune System Function and Inflammation: Relevance for Preclinical Studies

“…103 Diclofenac, indomethacin, and ketoprofen all exacerbated colitis in male Wistar rats, but celecoxib had no significant effect. 26 In BALB/c mice, tramadol administration did not affect inflammation, but buprenorphine was antiinflammatory. 24 BU08070, a buprenorphine analog, produced a concentration-dependent decrease in inflammation and visceral pain-induced behaviors in male BALB/c mice.…”