Pancreatite aguda experimental induzida pela L-arginina: avaliação histológica e bioquímica

Abstract

- Experimental acute pancreatitis induced by L-arginine leads to pancreatic necrosis showing self-limited evolution with pancreas regeneration in 2 weeks.

Ação do tacrolimus na pancreatite aguda experimental induzida pela arginina

“…As shown in figure 3, groups T and PT -treated with tacrolimus -had similar serum levels, which endorses the procedure and demonstrates the bioavailability of the drug. It is known that the increase of serum amylase does not correlate with the severity of acute pancreatitis and also with the histological changes observed in the pancreas 23,24 . In this study, as shown in figure 1, serum amylase levels remained normal in group C (1000 ± 87.1 U/L) but rose significantly in groups P (2577.5 ± 1501.5 U/ L) and PT (2788.7 ± 531.1 U/L), injected with L-arginine (p = 0.0000).…”

“…This non-invasive model proved to be easily reproduced and affordable when compared to invasive experimental models of acute pancreatitis 20 . The experimental model used herein displays, as its basic features, the elevation of serum amylase in the first 24 hours, with return to normal until the seventh day; it does not induce changes in glucose levels and the histological changes of the pancreatic parenchyma (edema and necrosis) are more severe in the period between 72 and 96 hours of onset 23 . As seen in figure 2, injection of L-arginine did not alter the endocrine function of the pancreas because the serum glucose levels were maintained in groups P, PT and T levels similar to those of group C. For this reason, in this study we chose the collecting samples within 96 hours of onset (fourth day) to coincide with the timing of more severe pancreatic histopathological changes, allowing time for the action of tacrolimus, which was administered daily during the four days of evaluation.…”

“…We considered the following histological patterns: edema, inflammatory infiltration, hemorrhage and parenchymal necrosis. The histologic pattern (Table 2) was defined according to the presence and predominance of microscopic changes 19., 23 .…”