Avaliação do teste rápido da urease conservado em geladeira

Ornellas, Laura Cotta; Cury, Marcelo; Lima, Vinícius Machado de; Ferrari, Ângelo Paulo

doi:10.1590/s0004-28032000000300003

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…These fragments were submitted to rapid urease test (URETEST, Renylab, PR) that comprises a qualitative colorimetric test to identify the bacterium. It is a highly specific and sensitive test, being more frequently used for the endoscopic diagnosis due to the potent urease activity of the bacterium (3,19) .…”

Section: Methodsmentioning

confidence: 99%

PREVALÊNCIA DE Helicobacter spp. EM CÃES DE CAMPO GRANDE-MS

et al. 2017

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Helicobacter spp. is a spiral Gram-negative bacterium that has substantial clinical importance. It has been related to diseases such as gastritis and peptic ulcers, and more recently to gastric cancer in humans. Evidence suggests the potential of animals, particularly domestic ones, as the source of zoonotic infection of helicobacteria since bacteria with similar morphology to those found in animals were observed in the stomach of humans with gastritis. Thus, dogs have been identified to serve as an important host for infectious agents such as Helicobacter spp. From this perspective, the present study aimed to assess the prevalence of Helicobacter spp. in dogs from the Zoonosis Control Center of Campo Grande-MS. Samples of body, fundus, and gastric antrum from 96 dogs were collected to evaluate the presence of Helicobacter spp. through the rapid urease test and histological analysis. Helicobacter spp. was found in 94.7% of the dogs by rapid urease test and in 100% by histological analysis, with bacteria predominance in the stomach fundus region. Keywords: dogs; Helicobacter; urease test. ResumoA Helicobacter spp. é uma bactéria Gram negativa espiralada, de grande importância clínica, que se relaciona a patogenias como gastrite e úlceras pépticas e, mais recentemente, com o carcinoma gástrico em humanos. Evidências sugerem o potencial dos animais, principalmente os domésticos, como fonte de infecção zoonótica das helicobactérias, já que bactérias com morfologia similar às encontradas em animais foram observadas no estômago de humanos com gastrite. Nesse contexto, os cães podem ser um importante reservatório de agentes infecciosos como a Helicobacter spp. O presente trabalho teve como objetivo avaliar a prevalência de Helicobacter spp. em cães do Centro de Controle de Zoonoses de Campo Grande/MS. Para tanto, foram utilizados 96 cães dos quais foram colhidas amostras do corpo, fundo e antro gástrico, para avaliação da presença da Helicobacter spp. por meio do teste rápido de urease e análise histológica. O teste rápido de urease permitiu a detecção de Helicobacter spp. em 94,7% dos cães; já a análise histológica indicou a presença de Helicobacter spp. em 100% dos animais avaliados com predominio da bactéria na 2

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Section: Methodsmentioning

confidence: 99%

PREVALÊNCIA DE Helicobacter spp. EM CÃES DE CAMPO GRANDE-MS

et al. 2017

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“…The most used are the rapid urease test and histological analysis [4]. The rapid urease test has a sensitivity of 92% and specificity of 95% [5]. Laineet al found histological sensitivity variation in the identification of Hpbacteria according to the bacterial density in the sample [6].…”

Section: Introductionmentioning

confidence: 99%

Endoscopic Findings Related To Helicobacter Pylori

2018

MCR

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Background: Studies with the latest technologies such as endoscopy with magnification and chromoendoscopy showed that various endoscopic aspects are clearly related to infection by Helicobacter pylori (Hp). The description of different patterns of erythema in gastric body under magnification of images revived interest in identifying these patterns by standard endoscopy. Aim: To validate the morphologic features of gastric mucosa to conventional gastroscopy Hprelated gastritis allowing predictability of their diagnosis as well as proper targeting biopsies. Methods: Prospective study of 339 consecutive patients with the standard videoendoscope image analysis were obtained, recorded and stored in a program database. These images were studied with respect to the presence or absence of HP, diagnosed by rapid urease test and/or by histological analysis. Were studied: a) normal mucosa appearance; b) mucosal nodularity; c) diffuse nonspecific erythemaor redness of antrum and body; d) mosaic pattern with focal area of hyperemia; e) erythema in streaks or bands (red streaks); f) raised erosions; g) flat erosions; h) fundic gland polyps. The main exclusion criteria were the use of drugs, Hp pre-treatment and other entities that could affect results. Results: Applying the exclusion criteria, were included 170 of the 339 patients, of which 52 (30.58%) were positive for Hp and 118 negative. On the positive findings, the most associated with infection were: antralnodularity (26.92%); raised erosion (15.38%) and mosaic pattern in the body (21,15%). On the negative group the normal appearance of the mucosa was 66.94%; red streaks in 9.32%; flat erosions 11.86%; and fundic gland polyps 11.86%. Conclusion: Endoscopic findings are useful in predicting the outcome, localization and targeting of biopsies in gastritis related to Helicobacter pylori infection.The most representative form of Hp related gastritis was the nodularity of the antralmucosa. Diffuse redness and mosaic pattern in the bodyare closely related to Hp infection.Raised erosions are suggestive but not specific to the infection. Normal-appearing forms, red streaks and fundic gland polyposis are related to the negativity of Helicobacter pylori infection. Flat erosions were not conclusive of the presence or absence of Hp.

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“…The most used are the rapid urease test and histological analysis [4] . The rapid urease test has a sensitivity of 92% and specificity of 95% [5] . Laine et al [6] found histological sensitivity variation in the identification of HP bacteria according to the bacterial density in the sample.…”

Section: Introductionmentioning

confidence: 99%

Conventional Videoendoscopy and Endoscopic Findings Related to Helicobacter Pylori

Gomes¹,

Skare²,

Internationals³

2017

JGDLF

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Background: Studies with the latest technologies such as endoscopy with magnification and chromo endoscopy showed that various endoscopic aspects are clearly related to infection by Helicobacter pylori (HP). The description of different patterns of erythema in gastric body under magnification of images revived interest in identifying these patterns by standard endoscopy. Aim: Validate endoscopic aspects related to HP found in conventional video gastroscopy, allowing the predictability of the diagnosis as well as proper targeting biopsies. Methods: Prospective study of 339 consecutive patients with the standard video endoscope image analysis were obtained, recorded and stored in a program database. These images were studied with respect to the presence or absence of HP, diagnosed by rapid urease test and/or by histological analysis. Were studied: a) normal mucosa appearance b) mucosal nodularity c) diffuse nonspecific erythema or redness of antrum and body d) mosaic pattern with focal area of hyperemia e) erythema in streaks or bands (red streaks) f) elevated (raised) erosions g) flat erosions h) fundic gland polyps. The main exclusion criteria were the use of drugs, HP pre-treatment and other entities that could affect results. Results: Applying the exclusion criteria, were included 170 of the 339 patients, of which 52 (30.58%) were positive for HP and 118 negative. On the positive findings, the most associated with infection were: antral nodularity (26.92%); raised erosion (15.38%) and mosaic pattern in the body (21.15%). On the negative group the normal appearance of the mucosa was 66.94%; red streaks in 9.32%; flat erosions 11.86%; and fundic gland polyps 11.86%. Conclusion: Endoscopic findings are useful in predicting the outcome, localization and targeting of biopsies in gastritis related to HP infection. The most representative form of HP related gastritis was the nodularity of the antral mucosa. The raised erosion and mosaic pattern in the body are suggestive but not specific to the infection. Normal-appearing forms, red streaks and fundic gland polyposis are related to the negativity of HP infection. The other forms were not conclusive of the presence of HP.

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Avaliação do teste rápido da urease conservado em geladeira

Abstract: We concluded that rapid urease test can be stored in refrigerator for up to 1 week, without loss of clinical applicability, and that can make the test even easier to use for routine Helicobacter pylori tests in a busy endoscopy unit.

Cited by 7 publications

References 7 publications

PREVALÊNCIA DE Helicobacter spp. EM CÃES DE CAMPO GRANDE-MS

PREVALÊNCIA DE Helicobacter spp. EM CÃES DE CAMPO GRANDE-MS

Endoscopic Findings Related To Helicobacter Pylori

Conventional Videoendoscopy and Endoscopic Findings Related to Helicobacter Pylori

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