Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Abstract

-Background -The clinical outcome of Helicobacter pylori infection has been associated with virulence factors. The presence of these factors is useful as molecular markers in the identification of the high risk for developing severe gastric pathologies. Objective -To correlate the presence of virulence markers cagA and bab2A of H. pylori in oral and gastric biopsy samples. Methods -An observational, prospective, descriptive, and cross-sectional study was carried out between September 2011 and September 2012. Patients suffering dyspepsia with indication for upper gastrointestinal video endoscopy who attended the Gastroenterology Service of the Hospital Dr. Julio C. Perrando were included. Epidemiological investigation was completed. To detect the bacteria and their virulence genes, samples of saliva, dental plaque and gastric biopsy were taken and processed by PCR.Results -Sixty-one patients were selected for this study (30 women and 31 men). H. pylori was detected in 31 gastric biopsies and 31 oral samples.Significant difference between oral and gastric samples was found in cagA genotype. Agreement between oral and gastric genotypes was found in 38.7% of samples from the same patient. Conclusion -This study is the first in provide information about the genotypes of the Argentinean Northeast H. pylori strains. Despite the high prevalence of H. pylori infection, the most of patients had less virulent genotypes in oral cavity and gastric tissue. The cagA/babA2 combination was not frequent in the samples studied. There was not a statistical correlation between the virulence genes and gastroduodenal or oral diseases. Although in some patients the same genotype was found both in oral and gastric samples, it cannot be ensure that they corresponding to the same strain because a DNA sequencing was not performed.

Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

“…H. pylori is a standout among the most widely recognized infectious agents around the world, and roughly half of the world's population is estimated to be infected [ 24 ]. Multiple diagnostic techniques are developed to detect H. pylori infection and are divided into two groups of invasive and noninvasive methods according to the necessity of endoscopic biopsy [ 25 ].…”

Helicobacter pylori Related Diseases and Osteoporotic Fractures (Narrative Review)

“…is a spiral-shaped, flagellated, microaerophilic, Gram-negative bacterium, which coevolved with humans >50,000 years [ 9 , 15 ]. The bacterium, discovered in 1982 by Warren and Marshall [ 16 , 17 ], colonizes the stomach in approximately 4.4 billion individuals [ 10 , 18 ] and is currently recognized as the most important microbiological agent in human upper gastrointestinal tract disorders. The prevalence of HPI (about 30% in developed countries and up to 80% in developing countries) varies by age (higher in the elderly, especially among institutionalized people); socioeconomic, urbanization and sanitation conditions; lifestyle and diet factors; and geographical regions (Central/South America, Asia, Eastern and Southern Europe have 50–80% higher prevalence than the rest of the world) [ 19 ].…”

Detection of Helicobacter pylori in oral yeasts from students of a Chilean university

“…There is evidence that H. pylori and Candida share habits in areas of the human anatomy, such as in the mouth, which is the anatomical site for the entry of microorganisms that colonize the digestive apparatus, in addition to the fact that both microorganisms can survive in the acidic environment of the stomach [11][12][13] , which would favor their interaction and the internalization of the bacterium in the yeast, which would grant it protection, nutrition, and a transmission medium that would allow the bacteria to survive outside the human gastrointestinal environment since yeast can survive in different environments, including in conditions with a lack of nutrients 2.1014-16 .…”