Aumento da expressão do MCP-1 coroidal e escleral em modelo experimental de hipercolesterolemia

Torres, Rogil José de Almeida; Noronha, Lúcia de; Casella, Antônio Marcelo Barbante; Lumikoski, Thaís Isabel; Précoma, Leonardo Brandão; Torres, Caroline Luzia de Almeida; Luchini, Andréa; Sturzeneker, Mário Cláudio Soares; Précoma, Dalton Bertolim

doi:10.1590/s0004-27492012000100010

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…In a recent experimental study, our group demonstrated that a hypercholesterolemic diet increased MCP-1 expression in the sclera and choroid, suggesting that the changes in great vessels that induce atherosclerotic plaque formation might also occur in the sclera-choroid complex (16) . After observing an increase in chemotactic molecules such as MCP-1, adhesion molecules had to be assessed to verify the hypothesis that atherosclerosis and AMD have similar pathophysiological mechanisms (17)(18)(19) .…”

Section: Discussionmentioning

confidence: 95%

Increased intercellular adhesion molecule-1 immunoreactivity in the sclera-choroid complex in hypercholesterolemia experimental model

Noronha¹,

Torres²,

Nagashima³

et al. 2014

Revista Brasileira De Oftalmologia

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Section: Discussionmentioning

confidence: 95%

Increased intercellular adhesion molecule-1 immunoreactivity in the sclera-choroid complex in hypercholesterolemia experimental model

Noronha¹,

Torres²,

Nagashima³

et al. 2014

Revista Brasileira De Oftalmologia

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Effect of flaxseed on choroid–sclera complex thickness and on LDL oxidation in the sclera, choroid and retina of diet-induced hypercholesterolaemic rabbits

Torres

Luchini²,

Both

et al. 2014

Br J Nutr

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The aim of the present study was to evaluate the effect of flaxseed on choroid -sclera complex thickness and on LDL oxidation in the sclera, choroid and retina of diet-induced hypercholesterolaemic rabbits. New Zealand male albino rabbits (n 21) were divided into two groups: group 1 (G1; n 11), fed a hypercholesterolaemic diet, and group 2 (G2; n 10), fed a hypercholesterolaemic diet enriched with flaxseed flour. The serum concentrations of total cholesterol (TC), LDL-cholesterol (LDL-C), HDL-cholesterol, TAG and fasting blood glucose were determined at the start of the experiment and on the day of killing (8th week). Choroid and sclera samples were subjected to haematoxylin -eosin (HE) staining and histomorphometric and immunohistochemical analyses with the anti-oxidised LDL antibody. Sensory retina samples were subjected to an immunohistochemical analysis with the primary monoclonal nitrotyrosine antibody. At the end of the experiment, a significant increase was observed in TC and LDL-C concentrations in G1 rabbits when compared with G2 rabbits (P¼0·008 and P¼0·02, respectively). HE staining revealed a significant increase in choroid -sclera complex thickness in G1 rabbits when compared with G2 rabbits (P,0·001). Immunohistochemical analysis of choroid and sclera samples with the anti-oxidised LDL marker revealed a significant increase in immunoreactivity in G1 rabbits when compared with G2 rabbits (P,0·001). Immunohistochemical analysis of sensory retina samples with the anti-nitrotyrosine marker revealed a significant increase in immunoreactivity in G1 rabbits when compared with G2 rabbits (P¼ 0·002). Flaxseed reduced the choroid -sclera complex thickness of diet-induced hypercholesterolaemic rabbits and the expression of oxidised LDL in the choroid -sclera complex as well as the expression of nitrotyrosine in the sensory retina.

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Aumento da expressão do MCP-1 coroidal e escleral em modelo experimental de hipercolesterolemia

Abstract: This study has revealed that the hypercholesterolemic diet in rabbits induces an increase in the MCP-1 expression in the choroid and sclera.

Cited by 2 publications

References 11 publications

Increased intercellular adhesion molecule-1 immunoreactivity in the sclera-choroid complex in hypercholesterolemia experimental model

Increased intercellular adhesion molecule-1 immunoreactivity in the sclera-choroid complex in hypercholesterolemia experimental model

Effect of flaxseed on choroid–sclera complex thickness and on LDL oxidation in the sclera, choroid and retina of diet-induced hypercholesterolaemic rabbits

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