Impression cytology features of conjunctival nevi reported as more noticeable

Barros, João; Lowen, Márcia Serva; Mascaro, Vera Lúcia Degaspare Monte; Andrade, Thais Piedade de; Martins, Maria Cristina

doi:10.1590/s0004-27492009000200014

Cited by 8 publications

(7 citation statements)

References 21 publications

(45 reference statements)

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“…16,17 The IC criteria for BPL such as nevi and primary acquired melanosis without atypia were the presence of nests or clusters of melanocytes containing cytoplasmic pigment and bland-looking nuclei, and the absence of mitosis (Figure 1(d)). 5,18,19…”

Section: Clinical and Cytopathological Criteria For The Diagnosis Of Bplmentioning

confidence: 99%

Impression cytology for detection of clinically suspected ocular surface disorders: A cross-sectional study

Moshtaghion

Abolhosseini

Kanavi

et al. 2020

European Journal of Ophthalmology

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Purpose: Investigating impression cytology (IC) results of various types of clinically suspected ocular surface lesions over a 14-year period in a referral center in Iran. Methods: IC findings obtained from patients with different types of ocular surface disorders between 2005 and 2018 were reviewed. Agreement between clinical suspicions and IC results was evaluated by calculating Cohen’s Kappa coefficient (CKC). Results: Clinical suspicions in 688 surveyed eyes were ocular surface squamous neoplasia (OSSN, 42.0%), limbal stem cell deficiency (LSCD, 36.3%), dry eye-related disorders (DERD, 11.5%), Acanthamoeba keratitis (AK, 7.2%), benign pigmented lesions (BPL, 1.9%), immune-related conjunctivitis (IRC, 0.7%), and malignant pigmented lesions (MPL, 0.4%). General agreement between clinical suspicions and IC results was 0.68 for all groups. This agreement was almost perfect in AK (CKC = 0.966) and BPLs (CKC = 0.843), and was substantial in MPLs (CKC = 0.749), OSSNs (CKC = 0.684), and LSCD (CKC = 0.612). CKC in IRC (0.567) and DERDs (0.443) was moderate. Histopathologic results were available in 22 eyes and were well-correlated with corresponding IC results (CKC = 0.86). Multiple post-treatment follow-up sessions of IC were performed in 51 eyes (11.4%) that had diagnosis of LSCD (31), OSSN (17), and MPL (3) at the first IC session. Conclusion: Our survey not only demonstrated an overall substantial agreement between IC results and primary clinical suspicions, but also showed an almost perfect correlation between IC results and existent histopathologic data. Therefore, IC as a non-invasive diagnostic modality can be of great importance in proper diagnosis of various ocular surface diseases especially when distinguishing malignant from benign lesions is required.

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Section: Clinical and Cytopathological Criteria For The Diagnosis Of Bplmentioning

confidence: 99%

Impression cytology for detection of clinically suspected ocular surface disorders: A cross-sectional study

Moshtaghion

Abolhosseini

Kanavi

et al. 2020

European Journal of Ophthalmology

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“…The simplicity of sampling of conjunctival areas other than corneoscleral limbus by using cellulose acetate strips has been previously reported[15] in comparison to sampling difficulties when Biopore membranes are used. [16]…”

Section: Discussionmentioning

confidence: 99%

“…[5] It could also discriminate the amelanotic melanocytic lesions from the non-pigmented non-melanocytic ones and confirm clinical diagnosis of 35 conjunctival nevi in 91.4%. [15] However, there is no report regarding the sensitivity and specificity of IC in the diagnosis of ocular surface melanoma or any other melanocytic lesion. Furthermore, given the superficial sampling nature of this technique, it has to be performed repeatedly to recover the melanocytic cells.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, although this method may not replace the gold standard mode of histopathology, it can play an essential role in the diagnosis and management of patients with ocular surface melanocytic lesions. [615] In the present study, the application of IC for the diagnosis of clinically diagnosed ocular surface melanocytic disorders was evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Impression cytology in a series of clinically diagnosed ocular surface melanocytic lesions

Kanavi

Hosseini

Aliakbar-Navahi

et al. 2017

J Ophthalmic Vis Res

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Purpose:To report impression cytology (IC) results of clinically diagnosed ocular surface melanocytic lesions.Methods:Ten patients with a clinical diagnosis of an ocular surface melanocytic lesion underwent IC using cellulose acetate strips and Periodic acid Schiff-Papanicolaou staining. Excisional biopsy of lesions was performed in case of observing atypical cells on IC or at the patient's request, and excised specimens were subjected to histopathological analysis. Agreement between clinical diagnoses and IC results and between IC results and histopathology were evaluated.Results:Clinical diagnoses were nevi in 6, primary acquired melanosis (PAM) with atypia/melanoma in 2, and atypical nevus versus pigmented conjunctival intraepithelial neoplasia (CIN) in 2 cases. IC results were suggestive of a benign nevus in 7, PAM with atypia/melanoma in 2 and CIN versus an atypical epithelioid type melanocytic lesion in 1 case. IC results were consistent with the clinical diagnoses in 9 cases (Cohen's kappa index of 0.83) and excluded CIN in 1. Histopathology in 6 cases disclosed benign melanonevus in 3, malignant melanoma in the context of PAM with atypia in 2, and CIN in 1 case. Histologic results were well correlated with the IC features (Cohen's kappa index of 0.74).Conclusion:By demonstrating typical cytomorphological features of ocular superficial layers IC diagnosed the true nature of melanocytic ocular surface lesions in the majority of cases. Although IC does not substitute histopathology, given the high correlation between IC results and histopathology, it can be of great assistance in diagnosis and management of ocular surface melanocytic lesions.

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“…IC confirmed the clinical diagnosis by demonstrating typical histopathological features of the superficial layers of conjunctival nevi in 91.4% of the cases. For amelanotic nevi, IC also allows differential diagnosis from other non-pigmented lesions (12) . Recently, a case of an amelanotic corneally displaced malignant conjunctival melanoma was described.…”

Section: Application Of Ic In the Evaluation Of Lesions Of Melanocytimentioning

confidence: 99%

Impression cytology in the evaluation of ocular surface tumors: review article

Barros¹,

Almeida²,

Lowen³

et al. 2015

Arquivos Brasileiros De Oftalmologia

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Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Information regarding this can be found in the English-language literature since 1992. Using either cellulose acetate or Biopore membranes for specimen collection, a high correlation has been found between IC and tissue histology. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye, provides a precise location of the area being studied, and allows accurate observation of the cells the way they exist in vivo. The additional advantage of IC is the preservation of limbal stem cells responsible for continuous corneal epithelium renewal; these can be affected after incisional or excisional biopsy at the corneoscleral limbus, which is the most frequent site of appearance of tumors in the stratified epithelium. Treatment for ocular surface squamous neoplasia has historically included surgery, but nonsurgical interventions have also been adopted. Hence, in certain cases, ophthalmologists may prefer interventions less invasive than surgical biopsy such as of impression cytology for both initial diagnosis and therapeutic monitoring of treatment for ocular surface lesions. Nevertheless, it should be considered that IC may be less helpful if the results conflict with the clinical picture or if the clinical diagnosis is uncertain and results are negative. In such cases, surgical biopsy is required for accurate diagnosis. The purpose of this review is to examine the published literature on the utilization of IC for the diagnosis and management of ocular surface tumors and to discuss the requirement for further investigation on the subject.

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Impression cytology features of conjunctival nevi reported as more noticeable

Cited by 8 publications

References 21 publications

Impression cytology for detection of clinically suspected ocular surface disorders: A cross-sectional study

Impression cytology for detection of clinically suspected ocular surface disorders: A cross-sectional study

Impression cytology in a series of clinically diagnosed ocular surface melanocytic lesions

Impression cytology in the evaluation of ocular surface tumors: review article

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