Autologous serum for ocular surface diseases

Quinto, Guilherme Goulart; Campos, Mauro; Behrens, Ashley

doi:10.1590/s0004-27492008000700010

Cited by 97 publications

(84 citation statements)

References 40 publications

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“…Because autologous serum incorporates anti-apoptotic activity, it is able to increase the antioxidant capacity of tissues and it contains growth factors. [26][27][28] Autologous serum has been used over the last two decades to promote the healing of epithelial and cartilaginous damage of both known and unknown aetiologies. 13,[29][30][31] At the same time, it includes various immunoglobulins (e.g.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic and protective effects of autologous serum in amikacin-induced ototoxicity

et al. 2017

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Objective: Possible therapeutic and protective benefits of intratympanic autologous serum application in amikacininduced ototoxicity were investigated.Methods: Twenty-four guinea pigs were separated equally into two groups: therapeutic (group A) and protective (group B). Transient evoked otoacoustic emissions were recorded before and after autologous serum application. Apoptotic cells were identified in the organ of Corti, spiral limbus and spiral ganglion by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling ('TUNEL') method.Results: Transient evoked otoacoustic emission responses at 1, 1.4 and 2.8 kHz improved without significance after autologous serum application in group A ( p > 0.05). A significantly protective effect of autologous serum was determined at 4 kHz in group B ( p < 0.05). There were significantly fewer apoptotic cells at the spiral limbus in the therapeutic and protective groups compared to the control group ( p < 0.05).Conclusion: Autologous serum may offer protection against ototoxicity-induced hearing loss, but it cannot restore hearing. Immunohistochemically, autologous serum significantly decreases activation of the intrinsic pathway of pro-apoptotic signalling in mesenchymal cells compared to neurons and neurosensory cells.

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Section: Discussionmentioning

confidence: 99%

Therapeutic and protective effects of autologous serum in amikacin-induced ototoxicity

et al. 2017

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“…1 Penggunaan serum otologus dalam bentuk tetes mata merupakan pendekatan terapeutik yang direkomendasikan untuk mengobati kerusakan permukaan okular seperti pada kerusakan epitelial mata yang menetap (persistent epithelial defect/PED) dan Keratoconjuctivitis sicca (KCS) atau yang sering disebut dengan penyakit mata kering (dry eye). 2 Sejak saat itu pengobatan dengan serum otologus diaplikasikan secara luas dalam bidang kedokteran modern seperti ortopedi, dermatologi, bedah plastik, dan kosmetika. [1][2][3] Pengobatan serum otologus memberikan hasil klinis yang lebih baik karena tidak menimbulkan reaksi alergi, memperkecil risiko terjadi reaksi immunologi secara sistemik, mencegah reaksi ketidakcocokan serta menghindari penularan penyakit.…”

Section: Pendahuluanunclassified

“…2 Sejak saat itu pengobatan dengan serum otologus diaplikasikan secara luas dalam bidang kedokteran modern seperti ortopedi, dermatologi, bedah plastik, dan kosmetika. [1][2][3] Pengobatan serum otologus memberikan hasil klinis yang lebih baik karena tidak menimbulkan reaksi alergi, memperkecil risiko terjadi reaksi immunologi secara sistemik, mencegah reaksi ketidakcocokan serta menghindari penularan penyakit. 1,3 Serum otologus tersebut mengandung faktor pertumbuhan seperti epidermal growth factor (EGF) dan transforming growth factor beta (TGF-β), fibronektin, vitamin E dan vitamin A. Serum otologus juga mengandung immunoglobulin seperti IgG, IgA, lisozim dan faktor suplemen yang bersifat bakterisida.…”

Section: Pendahuluanunclassified

Serum Otologus dan human Epidermal Growth Factor (hEGF) Mempercepat Proliferasi dan Migrasi Keratinosit pada Proses Re-Epitelisasi

Agung¹,

Maksum²,

Toto³

2016

mkb

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AbstrakPenggunaan serum otologus merupakan pendekatan terapeutik yang direkomendasikan untuk mengobati beberapa jenis penyakit yang bersifat kronis, menahun bahkan dapat bertahan seumur hidup. Serum otologus mengandung epidermal growth factor (EGF) yang dapat menstimulasi proses migrasi dan proliferasi keratinosit pada proses re-epitelisasi dalam penyembuhan luka. Penambahan hEGF pada serum otologus dilakukan sebagai upaya untuk mempercepat proses penyembuhan luka. Pengujian aktivitas dilakukan dengan mengukur proliferasi dan migrasi sel keratinosit menggunakan cell line HaCaT. Proliferasi sel diukur dengan metode Water Soluble Tetrazolium-8 (WST-8) dan migrasi sel diukur dengan metode Scratch Assay. Variasi konsentrasi hEGF yang ditambahkan adalah 0,5; 1; 5; 10; 25 dan 50 ng/mL. Terdapat perbedaan yang bermakna pada hasil proliferasi sel HaCaT yang ditambahkan hEGF dan konsentrasi optimal pada penambahan hEGF 25 ng/mL (p<0,05), sedangkan penambahan variasi konsentrasi hEGF pada serum otologus tidak menunjukkan perbedaan yang bermakna terhadap proliferasi sel HaCaT (p>0,05 Autologous Serum and human Epidermal Growth Factor (hEGF) Accelerate Keratinocyte Proliferation and Migration during Re-epithelialization Process AbstractAutologous serum is used as a therapeutic approach recommended to treat certain types of chronic diseases that can even last a lifetime. Autologous serum contains Epidermal Growth Factor (EGF), which can stimulate the migration and proliferation of keratinocytes in the re-epithelialization process in wound healing. The addition of hEGF on autologous serum is a part of efforts to accelerate the wound healing process. Tests were performed by measuring proliferation and migration activities of keratinocytes cells using HaCaT cell line. The cell proliferation was measured using Water Soluble Tetrazolium-8 (WST-8) method while the cell migration was measured using Scratch Assay method. Variations in the concentration of hEGF added were 0.5, 1, 5, 10, 25, and 50 ng / mL. There were significant differences in the results of cell proliferation in hEGF-added HaCaTcells and the optimum concentration was seen in 25 ng/mL hEGF group (p <0.05). On the contrary, the addition of various concentrations of hEGF in autologous serum resulted in no significant difference in HaCaT cell proliferation (p>0 , 05). The addition of hEGF and autologous serum showed a visible role in accelerating the pace of cell migration. Within 18 hours, the percentage of cell migration in HaCaT cells added by hEGF and autologous serum reaches 55-70% and then 65-80% within 24 hours while HaCaT cells that receive hEGF only only reaches 25-45% cell migration which increases to 35-70% within 24 hours. [MKB. 2016;48(4):205-10]

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“…[105][106][107] Soro autólogo tem sido utilizado porque contém vitaminas, diversos fatores de crescimento e fibronectina, que são importantes para a integridade da córnea e conjuntiva. 108,109 Soro autólogo é indicado para o tratamento da xeroftalmia grave associada à DECH-c ocular para renovar os componentes do filme lacrimal e da superfície ocular.…”

Section: Terapia Adjuvante Para Dech-c Com Envolvimento Oralunclassified

Diretrizes para o diagnóstico, classificação, profilaxia e tratamento da doença enxerto contra hospedeiro crônica

Bouzas¹,

Silva²,

Tavares³

et al. 2010

Rev. Bras. Hematol. Hemoter.

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Autologous serum for ocular surface diseases

Cited by 97 publications

References 40 publications

Therapeutic and protective effects of autologous serum in amikacin-induced ototoxicity

Therapeutic and protective effects of autologous serum in amikacin-induced ototoxicity

Serum Otologus dan human Epidermal Growth Factor (hEGF) Mempercepat Proliferasi dan Migrasi Keratinosit pada Proses Re-Epitelisasi

Diretrizes para o diagnóstico, classificação, profilaxia e tratamento da doença enxerto contra hospedeiro crônica

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